Development of a Quantitative Determination Method for Aluminum Ions in Adsorbed Drugs Using Atomic Absorption Spectrometry with Electrothermal Atomization

Makarishcheva, D D; Колесникова, О. Н.; Tregubova, V E; Ustinnikova, O. B.

doi:10.1007/s11094-022-02669-0

Cited by 6 publications

(4 citation statements)

References 5 publications

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“…One spectroscopy method is electrothermal atomic absorption (EAA), an analytical technique determining the concentration of metallic elements in liquid samples 2 . Electrothermal atomization using a graphite tube furnace as an atomizer is a powerful and advantageous technique in atomic absorption spectroscopy because it allows analysis of refractory and trace analyte elements 3 , uses smaller samples, and provides higher sensitivity, making it a preferred choice in many analyses 4 .…”

Section: Introductionmentioning

confidence: 99%

Detection of arsenic and lead ions in water through validation of the electrothermal atomic absorption method

Vargas-Tierras,

Morales-León,

Andi-Barrera

et al. 2024

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The validation of the electrothermal spectrometry method for Arsenic and lead determination in water samples guarantees the quality of analytical data. The study was carried out at AQLAB. A theoretical and technical investigation was applied to ensure the reliability and accuracy of the analytical method. Parameters such as INEN standards, Eurachem international standards, AQLAB internal documents and environmental standards were used to validate the process. Several readings were performed on different samples to make calibration curves for As and Pb, evaluate the method's linearity, and obtain information on the slope, intercept and determination coefficient. Statistical calculations were used to determine the limit of detection and quantification, repeatability, reproducibility, trueness and uncertainty. The results show that the hypotheses were fulfilled, calibration curves with determination coefficient higher than 0.995, repeatability standard deviation lower than 16%, calculated F < critical F (4.96), calculated t student < theoretical (2.23), trueness was between 100.29-110.18 and 99.64-107.92%, uncertainty was 20% range 0.005 to 0.10 mg/L and 15% range 0.01-0.20mg/L, limit of detection was 0.001 and 0.005 mg/L and limit of quantification was 0.01 and 0.02mg/L, respectively. Therefore, the validation method was robust and accurate. Keywords: water samples, analytical data, reliable results, Arsenic, Lead, validation.

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Section: Introductionmentioning

confidence: 99%

Detection of arsenic and lead ions in water through validation of the electrothermal atomic absorption method

Vargas-Tierras,

Morales-León,

Andi-Barrera

et al. 2024

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“…The consistency of atomization with F-AAS is not as good as using a graphite tube. [19] By using electrothermal atomization in terms of GF-AAS, these disadvantages mentioned for F-AAS can be circumvented. [20] To our knowledge, GF-AAS has not yet been evaluated for an Rb efflux assay.…”

Section: Introductionmentioning

confidence: 99%

A graphite furnace‐atomic absorption spectrometry‐based rubidium efflux assay for screening activators of the K_v7.2/3 channel

Bartz

Beirow

Wurm

et al. 2023

Archiv der Pharmazie

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For the characterization of K v 7.2/3 channel activators, several analytical methods are available that vary in effort and cost. In addition to the technically elaborate patch-clamp method, which serves as a reference method, there exist several medium to high-throughput screening methods including a rubidium efflux flameatomic absorption spectrometry (F-AAS) assay and a commercial thallium uptake fluorescence-based assay. In this study, the general suitability of a graphite furnace atomic absorption spectrometry (GF-AAS)-based rubidium efflux assay as a screening method for K v 7.2/3 channel activators was demonstrated. With flupirtine serving as a reference compound, 16 newly synthesizedcompounds and the known K v 7.2/3 activator retigabine were first classified as either active or inactive by using the GF-AAS-based rubidium (Rb) efflux assay. Then, the results were compared with a thallium (Tl) uptake fluorescence-based fluorometric imaging plate reader (FLIPR) potassium assay. Overall, 16 of 17 compounds were classified by the GF-AAS-based assay in agreement with their channel-activating properties determined by the more expensive Tl uptake, fluorescence-based assay. Thus, the performance of the GF-AAS-based Rb assay for primary drug screening of K v 7.2/3-activating compounds was clearly demonstrated, as documented by the calculated Z'-factor of the GF-AAS-based method. Moreover, method development included optimization of the coating of the microtiter plates and the washing procedure, which extended the range of this assay to poorly adherent cells such as the HEK293 cells used in this study.

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“…A higher temperature of the flame may cause additional thermal excitation leading to ionization interference, while a decrease in temperature may be accompanied by insufficient atomization. The latter results in less sensitivity [ 14 ] and is generally valid for atoms analyzed using AAS. However, a comparison of atomization using a flame and graphite furnace when analyzing magnesium also showed a higher sensitivity for GF AAS [ 15 ].…”

Section: Introductionmentioning

confidence: 99%

Development and Application of an Atomic Absorption Spectrometry-Based Method to Quantify Magnesium in Leaves of Dioscorea polystachya

Krüger,

Weng,

Baecker

2023

Molecules

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The Chinese yam (Dioscorea polystachya, DP) is known for the nutritional value of its tuber. Nevertheless, DP also has promising pharmacological properties. Compared with the tuber, the leaves of DP are still very little studied. However, it may be possible to draw conclusions about the plant quality based on the coloration of the leaves. Magnesium, as a component of chlorophyll, seems to play a role. Therefore, the aim of this research work was to develop an atomic absorption spectrometry-based method for the analysis of magnesium (285.2125 nm) in leaf extracts of DP following the graphite furnace sub-technique. The optimization of the pyrolysis and atomization temperatures resulted in 1500 °C and 1800 °C, respectively. The general presence of flavonoids in the extracts was detected and could explain the high pyrolysis temperature due to the potential complexation of magnesium. The elaborated method had linearity in a range of 1–10 µg L−1 (R2 = 0.9975). The limits of detection and quantification amounted to 0.23 µg L−1 and 2.00 µg L−1, respectively. The characteristic mass was 0.027 pg, and the recovery was 96.7–102.0%. Finally, the method was applied to extracts prepared from differently colored leaves of DP. Similar magnesium contents were obtained for extracts made of dried and fresh leaves. It is often assumed that the yellowing of the leaves is associated with reduced magnesium content. However, the results indicated that yellow leaves are not due to lower magnesium levels. This stimulates the future analysis of DP leaves considering other essential minerals such as molybdenum or manganese.

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Development of a Quantitative Determination Method for Aluminum Ions in Adsorbed Drugs Using Atomic Absorption Spectrometry with Electrothermal Atomization

Cited by 6 publications

References 5 publications

Detection of arsenic and lead ions in water through validation of the electrothermal atomic absorption method

Detection of arsenic and lead ions in water through validation of the electrothermal atomic absorption method

A graphite furnace‐atomic absorption spectrometry‐based rubidium efflux assay for screening activators of the K_v7.2/3 channel

Development and Application of an Atomic Absorption Spectrometry-Based Method to Quantify Magnesium in Leaves of Dioscorea polystachya

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Development of a Quantitative Determination Method for Aluminum Ions in Adsorbed Drugs Using Atomic Absorption Spectrometry with Electrothermal Atomization

Cited by 6 publications

References 5 publications

Detection of arsenic and lead ions in water through validation of the electrothermal atomic absorption method

Detection of arsenic and lead ions in water through validation of the electrothermal atomic absorption method

A graphite furnace‐atomic absorption spectrometry‐based rubidium efflux assay for screening activators of the Kv7.2/3 channel

Development and Application of an Atomic Absorption Spectrometry-Based Method to Quantify Magnesium in Leaves of Dioscorea polystachya

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A graphite furnace‐atomic absorption spectrometry‐based rubidium efflux assay for screening activators of the K_v7.2/3 channel