Development of a Quantitative Method for Detection of Multiclass Veterinary Drugs in Feed Using Modified QuPPe Extraction and LC–MS/MS

Abstract

A method was developed for the rapid and quantitative analysis of 30 veterinary drugs belonging to 17 classes (amphenicols (1), anthelmintics (1), cephalosporins (4), coccidiostats (1), lincosamides (1), macrolide (1), nitroimidazole (1), penicillins (3), phenylhydrazines (1), polypeptides (1), pyrethrins (1), quinolones (5), sulfonamides (3), tetracycline (3), neuroleptic agents (1), triazene trypanocidal agents (1), other. (1)) in feeds. The proposed method with a modified Quick Polar Pesticides (QuPPe) sample preparation was validated for the determination of 30 veterinary drugs in feed samples by liquid chromatography triple-quadrupole mass spectrometry (LC–MS/MS). The sample was extracted with methanol containing 1% acetic acid and purified by dispersive solid-phase extraction (d-SPE) with C18. Good linearity (r2 ≥ 0.98) was observed, and the LOQ values ranged from 10 to 200 µg/kg. Average recoveries ranged from 70.8 to 118.4%, and the relative standard deviation was ≤ 18.7%. This validated method was used in the determination of 30 veterinary drugs in 142 feed samples obtained from South Korea. The results show that lincomycin was present in only one of the tested feed samples, although it was detected at a value lower than the LOQ. In conclusion, this multi-residue method can be used for screening through the detection and quantitation of residual multiclass veterinary drugs in feed samples.

Deciphering and investigating fragment mechanism of quinolones using multi‐collision energy mass spectrometry and computational chemistry strategy

“…Quinolones are a type of antibacterial medicines used in the treatment of intestines, 23 cancer, 24 bacteria, 25 and parasites. 26 Many important MS methods and related studies focus on the qualitative/ quantitative determination of quinolones 27,28 and their derivatives. 29,30 A few studies have been conducted on fragmentation pathways and mechanism of quinolones.…”

Determination of Flunixin and 5-Hydroxy Flunixin Residues in Livestock and Fishery Products Using Liquid Chromatography-Tandem Mass Spectrometry (LC-MS/MS)

“…Plasma samples have been acidified using inorganic acids, such as hydrochloric acid, to precipitate proteins (Jedziniak et al, 2016). Quick, Easy, Cheap, Effective, Rugged, and Safe (QuEChERS) is the most common method for food sample preparation and is widely used for multi-class, multi-residue analyses (Hajrulai-Musliu et al, 2021;Jang et al, 2022;Wilkowska & Biziuk, 2011). To determine flunixin and 5hydroxy flunixin contents, high-performance liquid chromatography (HPLC)-ultraviolet (UV) spectrometry (Asea et al, 2001;Jedziniak et al, 2016), liquid chromatography (LC)-tandem mass spectrometry (MS/MS) (Daeseleire et al, 2003;Kruve et al, 2008;Malone et al, 2009), and other methods have been used.…”

Residual Behavior and Dietary Risk Assessment of Chlorfenapyr and Its Metabolites in Radish

“…Moreover, there are few reports on the detection of tralopyril. Liquid chromatography-mass spectrometry/mass spectrometry (LC-MS/MS) [ 27 , 28 , 29 , 30 ] and gas chromatography-mass spectrometry/mass spectrometry (GC-MS/MS) [ 31 , 32 ] can not only effectively separate and analyze compounds by chromatography, but also effectively qualitatively analyze substances by mass spectrometry. The complementary advantages of chromatography and mass spectrometry can be used for the qualitative and quantitative analysis of complex organic compounds.…”