Development of a radioimmunoassay for the measurement of Bisphenol A in biological samples

Kaddar, Nisrin; Bendridi, Nadia; Harthé, C.; Ravel, Marc Rolland de; Bienvenu, Anne‐Lise; Cuilleron, Claude-Yves; Mappus, Elisabeth; Pugeat, Michel; Déchaud, H.

doi:10.1016/j.aca.2009.04.036

Cited by 56 publications

(29 citation statements)

References 28 publications

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“…Although most intake occurs from ingestion, people who are engaged in the manufacturing or use of BPA (or related chemicals) can easily absorb it through the skin, or take it up via inhalation (He et al 2009b ;Kaddar et al 2009 ;Geens et al 2012 ).…”

Section: Sources and Routes Of Bisphenol A Exposurementioning

confidence: 99%

Adverse Effects of Bisphenol A on Male Reproductive Function

Manfo

Jubendradass

Nantia

et al. 2013

Reviews of Environmental Contamination and Toxicology

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BPA is a ubiquitous environmental contaminant, resulting mainly from manufacturing,use or disposal of plastics of which it is a component, and the degradation of industrial plastic-related wastes. Growing evidence from research on laboratory animals, wildlife, and humans supports the view that BPA produces an endocrine disrupting effect and adversely affects male reproductive function. To better understand the adverse effects caused by exposure to BPA, we performed an up-to-date literature review on the topic, with particular emphasis on in utero exposure, and associated effects on spermatogenesis, steroidogenesis, and accessory organs.BPA studies on experimental animals show that effects are generally more detrimental during in utero exposure, a critical developmental stage for the embryo. BPA has been found to produce several defects in the embryo, such as feminization of male fetuses, atrophy of the testes and epididymides, increased prostate size, shortening of AGD, disruption of BTB, and alteration of adult sperm parameters (e.g.,sperm count, motility, and density). BPA also affects embryo thyroid development.During the postnatal and pubertal periods and adulthood, BPA affects the hypothalamic-pituitary-testicular axis by modulating hormone (e.g., LH and FSH,androgen and estrogen) synthesis, expression and function of respective receptors(ER, AR). These effects alter sperm parameters. BPA also induces oxidative stress in the testis and epididymis, by inhibiting antioxidant enzymes and stimulating lipid peroxidation. This suggests that employing antioxidants may be a promising strategy to relieve BPA-induced disturbances.Epidemiological studies have also provided data indicating that BPA alters male reproductive function in humans. These investigations revealed that men occupationally exposed to BPA had high blood/urinary BPA levels, and abnormal semen parameters. BPA-exposed men also showed reduced libido and erectile ejaculatory difficulties; moreover, the overall BPA effects on male reproduction appear to be more harmful if exposure occurs in utero. The regulation of BPA and BPA-related products should be reinforced, particularly where exposure during the fetal period can occur. The current TDI for BPA is proposed as 25 and 50 1-1g/kg bwt/day (European Food Safety Authority and Health Canada, respectively). Based on the evidence available, we believe that a TDI value of 5 1-1g/kg bwt/day is more appropriate (the endpoint is modulation of rat testicular function). Certain BPA derivatives are being considered as alternatives to BPA. However, certain of these related products display adverse effects that are similar to those of BPA. These effects should be carefully considered before using them as final alternatives to BPA in plastic production.

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Section: Sources and Routes Of Bisphenol A Exposurementioning

confidence: 99%

Adverse Effects of Bisphenol A on Male Reproductive Function

Manfo

Jubendradass

Nantia

et al. 2013

Reviews of Environmental Contamination and Toxicology

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“…With an increase in the number of successful pregnancies that have resulted from assisted reproductive technologies, two studies examining unconjugated BPA levels in follicular fluid raise additional concerns (Ikezuki et al 2002;Kaddar et al 2009) (Table 5). First, Ikezuki et al (2002) found an average concentration of 2.4 ng/mL fluid collected from women undergoing in vitro fertilization (IVF) procedures.…”

Section: Healthymentioning

confidence: 99%

“…Seventeen studies have measured BPA in blood and serum samples from healthy male and nonpregnant female adults (Dirtu et al 2008;Fung et al 2000;He et al 2009;Hiroi et al 2004;Ikezuki et al 2002;Inoue et al 2000Inoue et al , 2001Kaddar et al 2009;Kuroda et al 2003;Liu et al 2007;Sajiki et al 1999;Sugiura-Ogasawara et al 2005;Takeuchi and Tsutsumi 2002;Takeuchi et al 2004;Volkel et al 2005;Yang M et al 2009;Yoshimura et al 2002) (Table 3). Of these studies, five used ELISA, one used a radioimmunoassay (RIA), and the remainder used analytical chemistry to assess BPA concentrations.…”

Section: Internal Dosage Assessment From Blood Measures Of Bpamentioning

confidence: 99%

“…First, Ikezuki et al (2002) found an average concentration of 2.4 ng/mL fluid collected from women undergoing in vitro fertilization (IVF) procedures. Second, Kaddar et al (2009) measured BPA in follicular fluid from 28 infertile women undergoing IVF. The authors detected unconjugated BPA in 11 of these samples (39%) at concentrations that ranged from 0.15 to 1 ng/mL fluid.…”

Section: Healthymentioning

confidence: 99%

“…In one of the larger studies of human blood, Kaddar et al (2009) examined plasma samples from 207 individuals, collected randomly in a French hospital, using an RIA (LOD = 0.08 ng/mL plasma; correlation with HPLC-MS, r 2 = 0.92). Unconjugated BPA was detected in 83% of the samples, and 12% had > 2 ng/mL plasma.…”

Section: Healthymentioning

confidence: 99%

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Urinary, Circulating, and Tissue Biomonitoring Studies Indicate Widespread Exposure to Bisphenol A

Vandenberg

Chahoud

Heindel³

et al. 2012

Ciênc. saúde coletiva

333

331

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Estudos de biomonitoração do sistema urinário, circulatório e tecidos indicam grande exposição ao Bisfenol A Resumo Bisfenol A (BPA) é um dos produtos quí-micos mais produzido em todo o mundo, e a exposição humana a ele é considerada onipresente. Assim, há preocupações de que a quantidade de BPA para o qual os seres humanos estão expostos podem causar efeitos adversos à saúde. Nós examinamos muitas possibilidades sobre o porquê estudos de biomonitorização e toxicocinética podem chegar a conclusões aparentemente conflitantes. Mais de 80 estudos publicados de biomonitorização humana que mediram a concentração de BPA em tecidos humanos, urina, sangue e outros fluidos, juntamente com dois estudos de toxicocinéti-ca do metabolismo humano BPA foram examinados. BPA não conjugado foi detectado no sangue (nonanogramas por mililitro gama), e BPA conjugado foi detectado na grande maioria das amostras de urina. Em contraste, estudos de toxico-cinética propuseram que os seres humanos não são internamente expostos ao BPA. Dados disponíveis de estudos de biomonitorização indicam que a população em geral está exposta ao BPA e em risco de exposição interna ao BPA não conjugado. Os dois estudos de toxicocinética, que sugeriram a exposição humana ao BPA é insignificante, têm deficiências significativas e estão diretamente refutados por outros estudos e, portanto não são confiáveis para fins de avaliação de risco. Palavras-chave Disruptor endócrino, Exposição humana, Modelo PBPK/PBTK, Gravidez, Avaliação de risco, Toxicocinética Abstract Bisphenol A (BPA) is one of the highest-volume chemicals produced worldwide, and human exposure to BPA is thought to be ubiquitous. Thus, there are concerns that the amount of BPA to which humans are exposed may cause adverse health effects. We examined many possibilities for why biomonitoring and toxicokinetic studies could come to seemingly conflicting conclusions. More than 80 published human biomonitoring studies that measured BPA concentrations in human tissues, urine, blood, and other fluids, along with two toxicokinetic studies of human BPA metabolism were examined. Unconjugated BPA was routinely detected in blood (in the nanograms per milliliter range), and conjugated BPA was routinely detected in the vast majority of urine samples (also in the nanograms per milliliter range). In stark contrast, toxicokinetic studies proposed that humans are not internally exposed to BPA. Available data from biomonitoring studies clearly indicate that the general population is exposed to BPA and is at risk from internal exposure to unconjugated BPA. The two toxicokinetic studies that suggested human BPA exposure is negligible have significant deficiencies, are directly contradicted by hypothesis-driven studies, and are therefore not reliable for risk assessment purposes.

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