Development of a rapid and sensitive LC–ESI/MS/MS assay for the quantification of propofol using a simple off-line dansyl chloride derivatization reaction to enhance signal intensity

Simultaneous determination of propofol and its glucuronide in whole blood by liquid chromatography–electrospray tandem mass spectrometry and the influence of sample storage conditions on the reliability of the test results

“…However, propofol is difficult to ionise, and the fragmentation efficiency is very weak. To improve the sensitivity, derivatisation of propofol prior to LC-MS/MS has been applied using dansyl chloride [14], 2-fluoro-1-methyl-pyridinum-p-toluene sulphonate [15] and a diazonium salt from aniline [16]. However, the specificity of the method is not necessarily improved by the derivatisation.…”

Spectrofluorimetric Method for the Determination of Tolterodine In Human Plasma and Pharmaceutical Preparations by Derivatization with Dansyl Chloride

“…TOL contains a phenolic hydroxyl group in its chemical structure that makes it suitable for derivatization using dansyl chloride. Dansyl chloride is an appropriate and widely used marker for fluorimetric derivatization (13)(14)(15)(16)(17). The new method is based on the derivatization of drug substance with dansyl chloride.…”

“…In this study, a new analytical method was developed based on the derivation of the TOL drug substance for the treatment of urinary incontinence using the dansyl chloride reagent. Dansyl chloride phenol is a commonly used fluorogenic derivatization indicator for the derivation of drugs containing primary and secondary amine groups (13)(14)(15)(16)(17). This reagent itself does not show fluorescence properties.…”

Fast liquid chromatography/tandem mass spectrometry determination of cannabinoids in micro volume blood samples after dabsyl derivatization

“…1) may present an opportunity for derivatization which ultimately could lead to improvement in the ESI process, particularly in acidic phase. Previous methods have used a dansyl derivatization step for amino acids [28,29], opiates [30] and other drugs [31], a dansyl derivatization could be use for cannabinoids. Forrest et al described the dansyl derivatization of cannabinoids in 1971, coupled to a thin layer chromatographic (TLC) separation [32].…”