Development of a rapid and inexpensive plasma glucose estimation by two-point kinetic method based on glucose oxidase-peroxidase enzymes

Basak, Anjan

doi:10.1007/bf02912902

Cited by 28 publications

(18 citation statements)

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“…In the current study, enzymatic method was used to test the samples: glucose oxidase (GOD) converts glucose into gluconic acid; Hydrogen peroxide formed in this reaction in presence of peroxide (POD), oxidatively couples with 4-aminoantipyrine/phenol to produce red quinoneimine dye. 16 This dye has absorbance maximum at 505 nm (500-550 nm). The intensity of colour is directly proportional to the glucose in specimen.…”

Section: Methodsmentioning

confidence: 99%

Prevalence of type two diabetes mellitus in Jammu rural

Kumar¹,

Badyal

2018

Int J Res Med Sci

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Background: Diabetes Mellitus (DM) represents one of the biggest challenges in our century, affecting hundreds of millions people worldwide, both in developed countries and in developing ones, as well; the main cause being an unhealthy life style: unhealthy food, lack of physical exercise, which most of the times, lead to obesity.Methods: This study was conducted to estimate the prevalence of DM in various age groups and gender by analyzing the hospital record based data from August 2017- December 2017 at Sub-District Hospital, Hiranagar, Kathua, J and K. Fasting plasma glucose ≥ 126 mg% and random plasma glucose ≥ 200 mg% were taken as the diagnostic criteria for diagnosis of DM.Results: Out of the 3600 patients screened for DM, majority were in the age group of 41-50 (37.06 %). Net prevalence was found to be 4.7% (170) of the total. Prevalence among females and males was found to be 49.41% and 50.59% respectively. The trend showed prevalence of approximately 6% in the age group of 41-50 and 51-60, but >60 years showed comparatively less prevalence i.e. <5%.Conclusions: Various studies from time to time have also discussed the most disturbing trend being the shift in age of onset of diabetes to a younger age in the recent years, which could be attributed to changing life-style, even in the rural areas, with a high percentage not knowing that they were suffering from diabetes; therefore, active screening programs are recommended in order to diagnose this condition earlier.

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Section: Methodsmentioning

confidence: 99%

Prevalence of type two diabetes mellitus in Jammu rural

Kumar¹,

Badyal

2018

Int J Res Med Sci

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“…The blood sample was divided equally into two tubes; Ethylene Diamine Tetra Acetic acid (EDTA) containing tube for the HbAlc and fluoride oxalate tube for FPG. The blood samples were coded and sent to the laboratory for the estimation of FPG by the glucose oxidase peroxidase colorimetric enzymatic method (14) and HbA1C by rapid desktop method. (15) Prediabetes was diagnosed when FPG was from 100-125 mg/dl and HbA1c was from 5.7%-6.4%.…”

Section: Anthropometric Measurementsmentioning

confidence: 99%

Effect of Health Education Program on Knowledge, Lifestyle and Health Profile of Prediabetic Employees in Zagazig University

Abdelsalam

Said

2018

Journal of High Institute of Public Health

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Background: Prediabetes is a risk factor for type 2 diabetes and cardiovascular diseases. So, its early detection and management are important. Objective(s): To identify the prediabetics among employees of Zagazig University. To assess the effect of a health education program on prediabetics' knowledge, lifestyle and health profile. Methods: Prediabetics were detected among 556 participants working in the managerial departments of Zagazig University, through two steps; firstly, screening by FINDRISC questionnaire then Fasting Plasma Glucose (FPG) and glycosylated haemoglobin (HBA1c) testing for those with FINDRISC scores of ≥ 15. The intervention was in the form of group education targeting adoption of a healthy lifestyle through providing knowledge and using behaviour change methods like goal setting, action planning and problem-solving with follow up for six months. Results: The participants with FINDRISC scores of ≥ 15 were 46.9%, only 21.9% of them were actually prediabetics. After the intervention, there was highly significant increase in the percent of participants with satisfactory knowledge and healthy lifestyle practices (p=0.00), and highly significant reduction in the mean value of FPG, HbA1c, body mass index and waist circumference. Conclusion: Health education was an effective tool that implicated a change in prediabetics' knowledge, lifestyle and health profile.

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“…Electrocardiogram was done to rule out coronary artery disease/atherosclerotic coronary vascular disease. Total serum cholesterol estimation was done using cholesterol oxidasephenol 4-aminophenazone peroxidase method [8]. Triglycerides (TGs) estimation was done by GPO-Trinder method [8].…”

Section: Research Articlementioning

confidence: 99%

“…Total serum cholesterol estimation was done using cholesterol oxidasephenol 4-aminophenazone peroxidase method [8]. Triglycerides (TGs) estimation was done by GPO-Trinder method [8]. Very low-density lipoprotein cholesterol (LDLc) and LDLc concentration estimation were done using Friedwald's formula.…”

Section: Research Articlementioning

confidence: 99%

Assessment of Cardiometabolic Risk Factors Among Local Population of National Capital Region

Sharma

Kumar

Sharma

et al. 2017

Asian J Pharm Clin Res

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Objective: In view of marked accentuations in cardiometabolic diseases in developing countries such as India, we aimed to conduct a cross-sectional study analyzing the prevalence of cardiometabolic risk factors in local population of Ghaziabad.Methods: The study is descriptive, random sample survey, and cross-sectional study. The study was carried out at Santosh Medical College, Ghaziabad, on 350 participants between the age group 18-70 years. After taking the informed consent, they were included in the study. The variables recorded were clinical history, all risk factors of cardiovascular disease including blood pressure, body mass index, waist circumference, blood sugar and serum lipid levels, cigarette use, and dietary habit.Result: Our study shows that the high prevalence of multiple risk factors in a general population with as many as 70% of the participants had metabolic syndrome. Among individually studied groups, obesity was the most common followed by dyslipidemia then diabetes and hypertension and the least prevalent was smoking. Females were more commonly affected than males by dyslipidemia and obesity, probably due to physical inactivity coupled with unhealthy diet and lifestyle.Conclusion: There is the higher prevalence of cardiometabolic risk factors among the population making the individuals prone to associated spontaneous coronary artery dissection and their predisposition. Therefore, timely detection of cardiometabolic factors and appropriate interventions may be of prime concern.

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Development of a rapid and inexpensive plasma glucose estimation by two-point kinetic method based on glucose oxidase-peroxidase enzymes

Abstract: A rapid and inexpensive plasma glucose estimation by two-point kinetic method based on glucose oxidase and peroxidase enzymes has been developed. It takes only 1½ minutes of time and validity of the method has been discussed.

Cited by 28 publications

References 1 publication

Prevalence of type two diabetes mellitus in Jammu rural

Prevalence of type two diabetes mellitus in Jammu rural

Effect of Health Education Program on Knowledge, Lifestyle and Health Profile of Prediabetic Employees in Zagazig University

Assessment of Cardiometabolic Risk Factors Among Local Population of National Capital Region

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