Development of a rapid and sensitive latex agglutination-based method for detection of group A rotavirus

Góes, A. C.; Moraes, Marcia Terezinha Baroni de; Silveira, Waldemir de Castro; Araújo, Irene Trigueiros; H’alluin, Jean-Claude de; Souza, Waldemar da Silva; Silva, José Godinho da; Leite, José Paulo Gagliardi

doi:10.1016/j.jviromet.2007.11.013

Cited by 7 publications

(4 citation statements)

References 22 publications

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“…Previously, rVP6 protein was used either for sero-diagnosis of RVA infection in humans [ 34 – 35 ] or for detection of human RVA antigen using anti-rVP6 antibodies in latex agglutination test format [ 36 ]. In latex-agglutination test, antibodies against the conserved N-terminal portion of the VP6 (1–245aa) displayed high sensitivity (98.5%) and specificity (100%), which were calculated by comparing with less sensitive RNA-PAGE [ 36 ]. Recently, another study utilized anti-rVP6 antibodies in differentiation of porcine rotaviruses from other porcine viruses, but lacked validation part of the assay [ 37 ].…”

Section: Discussionmentioning

confidence: 99%

Peptide-Recombinant VP6 Protein Based Enzyme Immunoassay for the Detection of Group A Rotaviruses in Multiple Host Species

et al. 2016

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We developed a novel enzyme immunoassay for the detection of group A rotavirus (RVA) antigen in fecal samples of multiple host species. The assay is based on the detection of conserved VP6 protein using anti-recombinant VP6 antibodies as capture antibodies and anti-multiple antigenic peptide (identified and constructed from highly immunodominant epitopes within VP6 protein) antibodies as detector antibodies. The clinical utility of the assay was evaluated using a panel of 914 diarrhoeic fecal samples from four different host species (bovine, porcine, poultry and human) collected from diverse geographical locations in India. Using VP6- based reverse transcription-polymerase chain reaction (RT-PCR) as the gold standard, we found that the diagnostic sensitivity (DSn) and specificity (DSp) of the new assay was high [bovine (DSn = 94.2% & DSp = 100%); porcine (DSn = 94.6% & DSp = 93.3%); poultry (DSn = 74.2% & DSp = 97.7%) and human (DSn = 82.1% & DSp = 98.7%)]. The concordance with RT-PCR was also high [weighted kappa (k) = 0.831–0.956 at 95% CI = 0.711–1.0] as compared to RNA-polyacrylamide gel electrophoresis (RNA-PAGE). The performance characteristics of the new immunoassay were comparable to those of the two commercially available ELISA kits. Our results suggest that this peptide-recombinant protein based assay may serve as a preliminary assay for epidemiological surveillance of RVA antigen and for evaluation of vaccine effectiveness especially in low and middle income settings.

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Section: Discussionmentioning

confidence: 99%

Peptide-Recombinant VP6 Protein Based Enzyme Immunoassay for the Detection of Group A Rotaviruses in Multiple Host Species

et al. 2016

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“…In routine microbiology laboratories of healthcare setting, diagnosis of rotavirus infection in stool samples is frequently detected by commercially accessible antigen kits, such as enzyme immunoassay (EIA) and latex agglutination (LA) tests that are highly specific. However, due to the levels of strain variation along with circulating gastrointestinal viruses, the sensitivity for many antigen kits is low [9].…”

Section: Introductionmentioning

confidence: 99%

Comparison of enzyme immunoassay, latex agglutination and polyacrylamide gel electrophoresis for diagnosis of rotavirus in children

El-Ageery

Ali

El-Khier

et al. 2020

Egyptian Journal of Basic and Applied Sciences

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Rotavirus is considered an important pathogen that causes diarrhea in children. Its laboratory identification is critical for early management and prevention of the outbreak. The aim of this study was to compare the three assays designed for diagnosis of rotavirus in stool samples: polyacrylamide gel electrophoresis (PAGE) as gold standard method, with the antigen detection method using enzyme immunoassay (EIA) and latex agglutination (LA). A cross-sectional retrospective study included 200 stool samples collected from children aged over 5 years with acute gastroenteritis. PAGE, EIA and LA techniques were used for the detection of rotavirus. LA showed higher percentage (36.5%), while EIA and PAGE were nearly equal (32% and 31.5%, respectively) in detection of rotavirus. Compared to PAGE, EIA presented 92.06%, 96.35%, 92.18 and 97.05 of sensitivity, specificity, positive and negative predictive values, respectively. The sensitivity, specificity, positive and negative predictive values of LA test were 84.13%, 85.40%, 72.60% and 92.12%, respectively. Antigen detection using EIA is sensitive, specific and rapid technique for detection of rotavirus infection in stool, while LA may be useful in disease outbreak, or with shortage of means for rotavirus recognition, since it is practical, fast, and reasonably priced.

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“…Many methods like reverse passive haemagglutination assay, enzyme immunoassay, complement fixation test and immunoelectrophoresis have been developed but are less sensitive. The most widely used methods for detection of rotavirus include latex agglutination, enzyme-linked immunosorbent assay (ELISA), lateral flow test and RNA-PAGE (de Góes et al 2008). Reverse transcriptase-polymerase chain reaction (RT-PCR) is now being extensively used as a confirmatory method for detection of rotavirus (Dewar et al 2005;Roman & Martinez 2005).…”

Section: Introductionmentioning

confidence: 99%

Comparison of agarose gel electrophoresis and RNA-PAGE for rapid detection of rotavirus from faecal samples

Dubal

Mawlong

Susngi

et al. 2014

Journal of Applied Animal Research

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Development of a rapid and sensitive latex agglutination-based method for detection of group A rotavirus

Cited by 7 publications

References 22 publications

Peptide-Recombinant VP6 Protein Based Enzyme Immunoassay for the Detection of Group A Rotaviruses in Multiple Host Species

Peptide-Recombinant VP6 Protein Based Enzyme Immunoassay for the Detection of Group A Rotaviruses in Multiple Host Species

Comparison of enzyme immunoassay, latex agglutination and polyacrylamide gel electrophoresis for diagnosis of rotavirus in children

Comparison of agarose gel electrophoresis and RNA-PAGE for rapid detection of rotavirus from faecal samples

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