Development of a rapid and simple voltammetric method to determine total antioxidative capacity of edible oils

Abstract

a b s t r a c tIn this work we report on a new, rapid and simple voltammetric method to determine the total antioxidant capacity (TAC) of the edible oils. The method explores the ABTS radical (2,2 0 -azinobis(3-ethylbenzothiazoline-6-sulphonic acid)) assay as a redox probe and it relays on measuring catalytic voltammetric currents. The electrocatalysis comprises redox regeneration of the electrochemically created ABTS Å+ radical either by Trolox (6-hydroxy-2,5,7,8-tetramethychroman-2-carboxylic acid) or by antioxidants present in studied oils. The detection limit of the method is determined to be 0.5 mg/L of Trolox equivalent, being a slightly lower than the corresponding UV-VIS spectrophotometric method. Applying the proposed voltammetric method the total antioxidant capacity of three types of commercially available cold-pressed edible oils are determined, and the results are found to be in a very good agreement with those obtained by UV-VIS spectrophotometry. The reported voltammetric method is cheap, rapid and simple, and it can be used as a sustainable alternative to the UV-VIS methods for the determination of total antioxidant capacitance of oils and other liquid lipophilic nutrients. Potent antioxidant capacity of studied oils was also confirmed by electron paramagnetic resonance spectroscopy of superoxide anion produced by macrophages.

Square‐Wave Voltammetry: A Review on the Recent Progress

“…Recently, the detection of antibiotic certiofur has been achieved by applying square-wave voltammetry directly in milk samples following the hydrolysis of certiofur [144]. Owing to its performances for determination of various polyphenolic antioxidants, useful and cheap SW voltammetric methods have been developed for the determination of the total antioxidative capacity in food products such as edible oils [145] and beverages [146]. Alkaloids are other class of natural compounds that attract considerable interest due to their wide range of applications in the pharmaceutical industry.…”

New insights into the chemistry of Coenzyme Q-0: A voltammetric and spectroscopic study

“…These phenomena are characterized by a gradient peak-current increase in the cyclic voltammograms, as the concentration of the hydroxyl derivatives of CoQ-0 (the antioxidants) increases. In addition, the voltammograms are characterized by a current elevation of the post-Faradaic current regions, which is a typical feature of catalytic regenerative mechanisms [33,37,38]. Since no absolute determination of the antioxidative potential is possible with this method, we used vitamin C to allow a comparison of the antioxidative ability of hydroxyl CoQ-0 derivatives relative to that of vitamin C. For that purpose, we performed an additional set of experiments in which we changed the concentration of vitamin C in the electrochemical cell.…”

Characterisation of traditional Macedonian edible oils by their fatty acid composition and their volatile compounds

“…Few studies focusing on the chemical composition of essential oils from various plant and herb species grown in Republic of Macedonia, such as Salvia officinalis populations, have been performed (Kostadinova et al, 2007;Najdoska, Bogdanov & Zdravkovski, 2010;Stefkov, Cvetkovikj, Karapandzova & Kulevanova, 2011). Only a preliminary study about fatty acid composition of vegetable oils and fats (Kostik, Memeti & Bauer, 2013) as well as one study concerning on antioxidative capacity determined by a voltammetric method (Gulaboski, Mirceski & Mitrev, 2013) are available in literature. However, detailed knowledge about the properties of these traditional oils is required in order to further improve their quality.…”