Development of a rapid and high frequency Agrobacterium rhizogenes mediated transformation protocol for Ocimum tenuiflorum

Vyas, Pritesh; Mukhopadhyay, Kunal

doi:10.2478/s11756-014-0375-7

Cited by 11 publications

(8 citation statements)

References 27 publications

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“…Mostly, young leaves of in vitro-germinated seedlings were used to infect with A. rhizogenes. Studies also reported the use of stems (Bais et al, 2002;Marzouk, 2009), as well as hypocotyl and cotyledon (Vyas and Mukhopadhyay, 2014;Srivastava et al, 2016b), for transformation purposes. Therefore, leaves from green variety of O. sanctum and Subja cultivar of O. basilicum are better to achieve more clones and higher biomass on MS media after treatment of cefotaxime or claforan.…”

Section: Media and Explant Used For Transformationmentioning

confidence: 99%

“…Strain ATCC 15834 ( Tada et al, 1996 ; Bais et al, 2002 ; Vyas and Mukhopadhyay, 2014 ) and strain LBA 9402 ( Skorupinska-Tudek et al, 2007 ; Marzouk, 2009 ) of A. rhizogenes were preferred during most studies to infect O. sanctum and O. basilicum . Other strains of A. rhizogenes used were MAFF 03-01724 ( Tada et al, 1996 ), A4, Arqua1-pTSC5, 8196, and 11325 ( Srivastava et al, 2016b , c ).…”

Section: Hairy Root Culture : Agrobacterium Rhizogenes -Mediated Transformationmentioning

confidence: 99%

“…Mostly, aerial parts of Ocimum have been reported with active biological components, including flavonoids, saponins, tannins, and triterpenoids in stems and leaves ( Pattanayak et al, 2010 ), as well as aldehydes, phenol, phenylpropanoids, and terpenes in seeds ( Mondal et al, 2009 ). A wide range of metabolites has been identified from Ocimum , including apigenin, apigenin-7-O-glucuronide, α-terpineol, β-bisabolene, bornyl acetate, camphene, campesterol, carnosic acid, carvacrol, caryophyllene, circimaritin, cirsilineol, α-elemene, eugenol, euginal, isothymusin, isothymonin, linalool, limatrol, luteolin, luteolin-7-O glucuronide, methyl chavicol, molludistin, myretenal, α- and β-pinenes, oleanlic acid, orientin, rosmarinic acid, sitosterol, stigmasterol, ursolic acid, and vicenin ( Gupta et al, 2002 ; Pattanayak et al, 2010 ; Shilpa et al, 2010 ; Baliga et al, 2013 ; Vyas and Mukhopadhyay, 2014 ; Wakchaure et al, 2017 ; Chaudhary et al, 2020 ; Singh et al, 2020 ).…”

Section: Introductionmentioning

confidence: 99%

“…O. sanctum is an erect, branched, annual herb with a square stem, which grows up to 18 inches (75 cm) into low bushes and has specific aroma ( Gupta et al, 2002 ; Prakash and Gupta, 2005 ; Mondal et al, 2009 ; Pattanayak et al, 2010 ; Shilpa et al, 2010 ; Rahman et al, 2011 ; Kulkarni and Adavirao, 2018 ). Therapeutic metabolic of O. sanctum is responsible for the defense mechanism of the plant ( Vyas and Mukhopadhyay, 2014 ). Antimicrobial properties of Tulsi have been utilized and available in the form of hand sanitizer, mouthwash, preservatives, and water purifier ( Cohen, 2014 ).…”

Section: Introductionmentioning

confidence: 99%

“…Vyas and Mukhopadhyay (2014) studied green and red varieties of O. sanctum, and (Srivastava et al, 2016b,c) analyzed three cultivars of O. basilicum (Subja, Holy Green and Red Rubin) for development of hairy root cultures.…”

mentioning

confidence: 99%

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Harnessing the Potential of Roots of Traditional Power Plant: Ocimum

2021

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Genus Ocimum of Labiatae is well known in all traditional medicinal systems like Ayurveda, Unani, Siddha, and Homeopathy. The pharmaceutical activities of different species of Ocimum attributed to all plant parts. Roots are the most significant vital organ of the plant, as they absorb water and nutrients from soil and transport to aerial parts of the plants. Roots of Ocimum were found helpful with free-radical scavenging activity to improve physical and mental strength as well as to treat diabetes, malaria, and liver problems. Antibacterial activity of Ocimum roots and its main component, rosmarinic acid, is very beneficial to protect against several human pathogens, including bacteria and viruses. Being so important in every way, roots of Ocimum need healthy rhizosphere. Bacteria, fungi, nematodes, types of soil, fungicide, pesticides, salt, radioactive elements, as well as heavy metal contaminations, affect roots and overall growth of Ocimum in positive or negative ways. Each component of rhizosphere (natural, treatment or contamination) affects the roots, which highlights current ecological scenario to discover biosafe and more productive approaches. For such prestigious organ of Ocimum, development of in vitro root cultures and hairy root cultures assists to reduce the efforts and timing of the traditional cultivation process along with elimination of negative factors in rhizosphere. Different strains of Agrobacterium rhizogenes, various media compositions, as well as discrete treatments, like elicitors, on nonidentical species or cultivars of Ocimum boost the root induction, biomass, and accumulation of phytoceuticals differently. Hairy roots and in vitro roots of Ocimum accumulate higher quantity of therapeutic metabolites. These metabolites include several phenolics (like rosmarinic acid, 3-hydroxybenzoic acid, m-coumaric acid, p-coumaric acid, caffeic acid, ferulic acid, vanillic acid, chicoric acid, and lithospermic acid), triterpenes (such as betulinic acid, 3-epimaslinic acid, alphitolic acid, euscaphic acids, oleanolic acid, and ursolic acid) as well as flavonoids (flavones, flavonols, and dihydroflavonols). This review highlights pharmaceutical applications of Ocimum roots, a great deal of rhizosphere components and in vitro culturing techniques to enhance biomass as well as chief phytoceuticals.

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