Development of a rapid diagnostic test based on loop-mediated isothermal amplification to identify the most frequent non-typhoidal Salmonella serovars from culture

Edel, Birgit; Glöckner, Stefan; Stoll, S.; Lindig, Nora; Boden, Katharina; Wassill, Lars; Simon, Sandra; Löffler, Bettina; Rödel, Jürgen

doi:10.1007/s10096-023-04571-3

Cited by 6 publications

(2 citation statements)

References 33 publications

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“…The advantages of the LAMP method are significant and do not require expensive reagents and instrument analysis [68]. This method was able to distinguish four Salmonella serovars, Salmonella Enteritidis, Salmonella Typhimurium, Salmonella Infantis, Salmonella Derby, and Salmonella choleraesuis with different gene targets (Transcriptional regulator, RHS repeat protein, Putative isochorismate, YadA-like family protein, and Predicted antirepressor, respectively) [69]. 5.9.…”

Section: Nested Pcrmentioning

confidence: 99%

Advancements in DNA analysis for distinguishing wild boar and domestic pig: a mini-review on research developments for food authentication studies

Volkandari,

Rohman,

Jenie

et al. 2024

IOP Conf. Ser.: Earth Environ. Sci.

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Ensuring food authenticity is integral to quality control and food safety, demanding traceability of food ingredient origins to be prominently featured on labels. Pork, a widely consumed and processed animal protein source, is susceptible to adulteration with wild boar meat, primarily driven by factors such as production costs and profitability. Complicating matters, the quality of wild boar meat obtained through game hunting lacks guarantees for hygienic standards, posing potential harm to consumers. Recent outbreaks of African Swine Fever in pigs and wild boars further emphasize the critical link between food safety and meat authenticity. This study addresses the need for research in distinguishing between pork and wild boar meat to uphold food quality control standards. Establishing the meat origin is not only crucial for quality control but also vital for mapping and tracing meat distribution patterns. The study adopts a DNA-based approach, utilizing biomarkers to distinguish domestic pig and wild boar meat. However, researchers encounter significant challenges due to their shared species (Sus scrofa) and high DNA sequence similarity. The pursuit of robust biomarkers and analytical methods becomes imperative. Exploring both nuclear and mitochondrial DNA, this mini-review outlines the research developments in identifying resilient biomarkers and employing various analytical methods, including conventional PCR, PCR-RFLP, Real-time PCR, microsatellite (STR), and deep sequencing (NGS or GWAS). The paper provides a concise overview of the ongoing efforts to find effective biomarkers and analytical methods based on DNA approaches for distinguishing domestic pigs and wild boars, showcasing their potential applications in food authentication.

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Section: Nested Pcrmentioning

confidence: 99%

Advancements in DNA analysis for distinguishing wild boar and domestic pig: a mini-review on research developments for food authentication studies

Volkandari,

Rohman,

Jenie

et al. 2024

IOP Conf. Ser.: Earth Environ. Sci.

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“…More than 2,600 distinct Salmonella serotypes have been identified to date. The vast majority of potentially fatal infections in people are caused by pathogenic strains of bacteria or viruses that contaminate their food [16,17]. The two main types of Salmonella genotypes are Typhoid salmonellae (TS) and Non-typhoidal salmonellae (NTS).…”

Section: Introduction 11 Overviewmentioning

confidence: 99%

Typhoidal Salmonellosis (TS) (Enteric fever)

Almashhadany,

Ahmood,

Omar

et al. 2023

ijmst

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The incidence of salmonellosis continues to rise, and it is one of the fastest spreading bacteria in the globe. It's responsible for the vast majority of bacterial infections in animals everywhere. Enteric fever is a worldwide health issue, especially prevalent in places with poor sanitation that allow for the contamination of food and water sources with human feces. This illness is also known as Typhoid fever. The neuropsychiatric effects of untreated typhoid were so severe that the disease was given a name taken from the ancient Greek word for cloud. It's a public health problem, especially in poor areas, because it can affect multiple body systems and kill you. Salmonella typhi and Salmonella paratyphi are the bacteria responsible for this disease. Typhoid fever and paratyphoid fever are both types of enteric fever. Worldwide, approximately 21 million individuals contract typhoid each year. Every year, it accounts for about 200,000 fatalities around the world. Its tolerance to antibiotics has grown over time. Salmonella is a genus of Enterobacteriaceae that can cause a wide range of illnesses in the digestive tract. Antibiotic resistance is a growing problem, and even though these diseases have been eliminated in most industrialized countries, they are still a major cause of death and disability worldwide. Typhoid and paratyphoid fever can be mitigated through the sanitation of potable water, but they cannot be eradicated without the control of human carriers as well. Nontyphoidal Salmonella serovars, which cause only mild cases of gastroenteritis in people, are genetically related to typhoidal Salmonella serovars.

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Evaluation of diagnostic accuracy of the wabG gene based Klebsiella pneumoniae detection by loop-mediated isothermal reaction in neonatal blood sample

Banerjee,

Prasad

et al. 2024

Diagnostic Microbiology and Infectious Disease

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Development of a rapid diagnostic test based on loop-mediated isothermal amplification to identify the most frequent non-typhoidal Salmonella serovars from culture

Cited by 6 publications

References 33 publications

Advancements in DNA analysis for distinguishing wild boar and domestic pig: a mini-review on research developments for food authentication studies

Advancements in DNA analysis for distinguishing wild boar and domestic pig: a mini-review on research developments for food authentication studies

Typhoidal Salmonellosis (TS) (Enteric fever)

Evaluation of diagnostic accuracy of the wabG gene based Klebsiella pneumoniae detection by loop-mediated isothermal reaction in neonatal blood sample

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