Development of a Rapid Fluorescent Immunochromatographic Test to Detect Respiratory Syncytial Virus

Trinh, Thuy-Tien Thi; Park, Hyun; Tuong, Hien Thi; Yu, Seung-Taek; Choi, Da-Hye; Yeo, Seon-Ju

doi:10.3390/ijms19103013

Cited by 7 publications

(8 citation statements)

References 36 publications

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“…We used enzyme-linked immunosorbent assay to confirm the antibody titer in mouse serum and produced cell lines that secrete McAbs using a cell-fusion technique described previously [41]. Splenocytes were extracted from a selected immune mouse and fused with myeloma cells (F/0 cell line) at a ratio of 1:5 to 1:10 in 50% polyethylene glycol solution (Sigma-Aldrich, St. Louis, MO, USA) and seeded in each well of a 96-well culture plate.…”

Section: Cell Fusion and Hybridoma Cell Cloningmentioning

confidence: 99%

“…IFA was performed as described previously [41,42]. MDCK cell monolayers were grown on glass coverslips placed in 6-well plates and infected with H1N1 virus, H9N2 virus at MOI 0.01 in DMEM containing 1% antibiotic, 1 µg/mL N-tosyl-L-phenylalanine chloromethyl ketone (TPCK-treated trypsin, Sigma-Aldrich, St. Louis, MO, USA).…”

Section: Immunofluorescence Assay (Ifa)mentioning

confidence: 99%

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Development of a Rapid Fluorescent Diagnostic System to Detect Subtype H9 Influenza A Virus in Chicken Feces

Tuong

Jeong

Choi

et al. 2021

IJMS

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The circulation of the H9N2 virus results in significant economic losses in the poultry industry, and its zoonotic transmission highlights the need for a highly sensitive and rapid diagnostic and detection system for this virus. In this study, the performance of lateral flow test strips for a fluorescent immunochromatographic test (FICT) was optimized for the diagnosis of H9N2 virus-infected animal samples. The novel monoclonal antibodies (McAbs) against influenza A H9 viruses were developed, and two categories of McAbs with linear and conformational epitopes were compared for the performance of rapid diagnostic performance in the presence of feces sample at different time points (2, 4, and 6 days) post-infection (dpi). The limit of detection (LOD) of FICT and Kd values were comparable between linear and conformational epitope McAbs. However, superior performance of linear epitope McAbs pairs were confirmed by two animal studies, showing the better diagnostic performance showing 100% relative sensitivity in fecal samples at 6 dpi although it showed less than 80% sensitivity in early infection. Our results imply that the comparable performance of the linear epitope McAbs can potentially improve the diagnostic performance of FICT for H9N2 detection in feces samples. This highly sensitive rapid diagnostic method can be utilized in field studies of broiler poultry and wild birds.

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Section: Cell Fusion and Hybridoma Cell Cloningmentioning

confidence: 99%

Section: Immunofluorescence Assay (Ifa)mentioning

confidence: 99%

Development of a Rapid Fluorescent Diagnostic System to Detect Subtype H9 Influenza A Virus in Chicken Feces

Tuong

Jeong

Choi

et al. 2021

IJMS

Self Cite

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“…The current detection methods for RSV involve fluorescent material or plasmon, which improve immunoassay sensitivity. 28,29 In the authors' previous work, 22 europium nanoparticles were employed as the fluorescent material to develop a rapid fluorescent immunochromatographic strip test (FICT) for detection of RSV, and the FICT showed superior sensitivity, at 90%, and specificity, at 98.18%. Although the fluorescent antibody labeling techniques are already commercially available and present high sensitivity and specificity, the dielectric spectroscopy technique is still a complementary and alternative method, in terms of label- free techniques, and the technique enables the measurement of biological materials submerged in a liquid.…”

Section: Discussionmentioning

confidence: 99%

“…The nucleoprotein (NP) gene of the RSV (GenBank: KT992094.1) was cloned and the RSV recombinant nucleoprotein (RSV rNP) was expressed in Escherichia coli and purified as previously described. 22 The monoclonal antibody targeting the RSV rNP developed in a previous work 12 was used in this study. As shown the inset in Figure 5, the three different samples of BSA, RSV rNP, and RSV rNP + antibody were prepared at 100 μg/1 mL in a 2 mL plastic vial.…”

Section: Preparation Of Aqueous Biological Materialsmentioning

confidence: 99%

Dielectric spectroscopy technique for detection of human respiratory syncytial virus

Cho

Trinh

Yeo

2019

Micro & Optical Tech Letters

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Human respiratory syncytial virus (RSV) can be serious for infants and older adults, though it is considered a common respiratory virus for most people. Rapid and sensitive detection of RSV is important for minimizing infection, and an effective diagnostic method is still in demand. Dielectric spectroscopy is a technique for measuring the complex permittivity spectra of a material‐under‐test (MUT). The spectra can provide a unique fingerprint of the MUT for higher detection accuracy. In this article, a dielectric spectroscopy technique, which uses an open‐ended coaxial probe submerged in aqueous biological materials, is presented for the detection of RSV. From the reflection coefficients at the end of an open‐ended coaxial probe, complex permittivity spectra over the frequency range of 0.1‐26.5 GHz are extracted and used to characterize the three different biological materials: RSV, RSV + an antibody targeting the RSV, and bovine serum albumin (BSA), which is used as a negative control sample in this article. It is shown that the RSV and RSV + antibody can be differentiated from BSA by three distinct features: (a) the real part of the complex permittivity spectra, (b) the ionic loss characteristic below 1 GHz, and (c) the relaxation frequency. These three features enable us to identify the presence of RSV in an aqueous biological material.

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“…Compared to methods such as ELISA, conventional ICT, which uses colloidal gold as the signal lable, requires a higher analyte load to produce a visible line to the naked eye ( Koczula and Gallotta, 2016 ). In order to overcome these limitations, it is recommended to introduce new labeling methods, such as fluorescence ( Thuy Tien et al., 2018 ), nanosphere ( Chen et al., 2020b ), and quantum dots (QDs) ( Lu et al., 2019 ), when developing new ICT methods. Considering the high false-negative rate of ICT, it is more suitable for rapid screening of suspected cases.…”

Section: Immunoassay Methodsmentioning

confidence: 99%

Recent advances in immunoassay technologies for the detection of human coronavirus infections

Wang

Chen

Xiang

et al. 2023

Front. Cell. Infect. Microbiol.

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Severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) is the seventh coronavirus (CoV) that has spread in humans and has become a global pandemic since late 2019. Efficient and accurate laboratory diagnostic methods are one of the crucial means to control the development of the current pandemic and to prevent potential future outbreaks. Although real-time reverse transcription-polymerase chain reaction (rRT-PCR) is the preferred laboratory method recommended by the World Health Organization (WHO) for diagnosing and screening SARS-CoV-2 infection, the versatile immunoassays still play an important role for pandemic control. They can be used not only as supplemental tools to identify cases missed by rRT-PCR, but also for first-line screening tests in areas with limited medical resources. Moreover, they are also indispensable tools for retrospective epidemiological surveys and the evaluation of the effectiveness of vaccination. In this review, we summarize the mainstream immunoassay methods for human coronaviruses (HCoVs) and address their benefits, limitations, and applications. Then, technical strategies based on bioinformatics and advanced biosensors were proposed to improve the performance of these methods. Finally, future suggestions and possibilities that can lead to higher sensitivity and specificity are provided for further research.

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Development of a Rapid Fluorescent Immunochromatographic Test to Detect Respiratory Syncytial Virus

Cited by 7 publications

References 36 publications

Development of a Rapid Fluorescent Diagnostic System to Detect Subtype H9 Influenza A Virus in Chicken Feces

Development of a Rapid Fluorescent Diagnostic System to Detect Subtype H9 Influenza A Virus in Chicken Feces

Dielectric spectroscopy technique for detection of human respiratory syncytial virus

Recent advances in immunoassay technologies for the detection of human coronavirus infections

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