Development of a Rapid, Sensitive and Specific Method for the Measurement of Aldosterone by Radioimmunoassay

Banks, P.; Ekins, Roger; Slater, J. D. H.

doi:10.1530/acta.0.067s094

Cited by 6 publications

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“…Radioimmunoassay methods consist of simple extraction of plasma with organic solvent followed by minimal purification of the residue before assay. The 'purification' may consist of chromatography on paper (Mayes et al, 1970;Bayard, Beitins, Kowarski & Migeon, 1970), Sephadex LH 20 (Ito, Woo, Haning & Horton, 1972) or thin layers of silica gel (Banks, Ekins & Slater, 1971). Such procedures do not achieve total purification and methodological specificity may depend largely on the quality of the antiserum employed in the assay.…”

Section: Discussionmentioning

confidence: 91%

A Comparison of Double-Isotope Derivative and Radioimmunological Estimation of Plasma Aldosterone Concentration in Man

1973

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S U M M A R Y1. Two techniques for estimating plasma aldosterone concentration are compared by means of repeated assays of a plasma pool and also by analysis of a wide range of plasma samples.2. No significant difference was found in the results obtained by the methods. Radioimmunoassay required only one tenth of the volume of plasma needed for the double-isotope derivative method.3. Its rapidity and relative inexpensiveness makes radioimmunoassay at present the most suitable technique for large-scale population screening.Key words: aldosterone, plasma, radioimmunoassay, double-isotope derivative assay. Radioimmunoassay techniques for steroid assay are considerably more sensitive than the majority of physiochemical methods but may be less specific. One means of assessing such a quality is to compare the technique with another which has already been extensively validated. A range of plasma samples varying widely in source and in aldosterone concentration were therefore assayed both by a simple radioimmunoassay method and by a double-isotope derivative method (see below) and the results compared.

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