Development of a rapid, simple and sensitive HPLC-FLD method for determination of rhodamine B in chili-containing products

Qi, Peng; Lin, Zhihao; Li, Jiaxu; Wang, Chenglong; Meng, Weiwei; Hong, Hong; Zhang, Xuewu

doi:10.1016/j.foodchem.2014.05.036

Cited by 77 publications

(44 citation statements)

References 5 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The G‐SERS substrate was then applied for the quantitative detection of RhB molecules, which is a synthetic dye with a bright rose color and used in the industries of paper and leather, but is forbidden as an additive in food production due to carcinogenic risk . RhB solutions with a series of concentrations in pure water were first measured (see representative G‐SERS spectra in Figure S8, Supporting Information) and the working curve ( I R vs c ) was plotted in Figure c.…”

Section: Resultsmentioning

confidence: 99%

In Situ Quantitative Graphene-Based Surface-Enhanced Raman Spectroscopy

et al. 2017

View full text Add to dashboard Cite

the uncertainty of the amount of detected molecules. The spectral instability is mainly due to the orientation fluctuation of molecules and/or the possible chemical reactions between the molecules and the metal substrate. [12][13][14] In principle, this can be avoided by isolating the molecules from the metal surface, for example, by coating a dielectric nanoshell on the metal nanostructures. [6,14,15] The inhomogeneous electromagnetic enhancement can be largely calibrated by implementing an internal standard species that experiences the same enhancement as the analyte molecule, so that the ratio between the Raman intensities of the analyte and the internal standard can be used for quantification. [6,16,17] The uncertainty of the amount of detected molecules is the most notorious because there have been few solutions so far, and the rough estimation of the number of molecules based on the homogenous-adsorption assumption and the detection area/volume has been generally adopted, although the probability of molecules adsorption is different on different facets of the metal nanostructures.Herein, we report a graphene-based SERS (G-SERS) substrate for analyte quantification that solves the above problems to a great extent. Graphene has shown its unique characteristics in SERS applications. [18][19][20][21] For example, graphene quenches the fluorescence of fluorophores and provides stable and clean Raman signals due to the separation of molecules from a metal. [18,19] Graphene has also been used to cover the surface of plasmonic nanostructures and the composite substrate can provide remarkable Raman enhancement. [22,23] More importantly, the single-crystalline nature of graphene guarantees that the probe molecules are homogeneously adsorbed on the surface, rendering the possibility of reliable determination of the number of molecules. Meanwhile, graphene is naturally an internal standard for the normalization of the Raman signals of analytes, so that the different enhancement experienced by molecules at different locations can be calibrated. In our substrate, a roughened metal film under graphene ensures that the overall Raman enhancement is still dominated by the sizable electromagnetic enhancement. We demonstrated in situ quantitative detection of crystal violet (CV) and rhodamine B (RhB) molecules in aqueous solutions with concentrations from 10 −8 to 10 −5 m and the real-time monitoring of a release process of RhB molecules through a permeable membrane. Our graphene-based SERS substrate promises SERS quantification Quantitative surface-enhanced Raman spectroscopy (SERS) with ultrahigh sensitivity will significantly promote its practical application in many fields, such as environment monitoring, food safety, and drug detection. However, the challenges that remain unresolved, particularly in the low concentration levels, arise from the instability of the SERS spectra and the uncertainty of the number of detected molecules. Herein, a graphene-based, flexible, and transparent substrate for SERS quantification is rep...

show abstract

Section: Resultsmentioning

confidence: 99%

In Situ Quantitative Graphene-Based Surface-Enhanced Raman Spectroscopy

et al. 2017

View full text Add to dashboard Cite

show abstract

“…Currently, enrichment and measurement of trace amounts of rhodamine B in complex food matrices is still a demanding task, requiring extensive pretreatment to extract and purify the title analytes prior to analysis. The analytical methods most commonly used for the determination of rhodamine B are liquid chromatograph-mass spectrometer (LC-MS) [4], high performance liquid chromatography (HPLC) [5], UV-vis spectroscopy [6], and fluorescence spectrometry [7,8], which are combined with sample pretreatment methods, such as liquid-liquid extraction (LLE), solid-phase extraction (SPE), etc. Since classical SPE sorbents (C8, C18, etc.)…”

Section: Introductionmentioning

confidence: 99%

Nanosilica-based molecularly imprinted polymer nanoshell for specific recognition and determination of rhodamine B in red wine and beverages

Long

Lü

et al. 2016

Journal of Chromatography B

View full text Add to dashboard Cite

“…然而, 一些不法商贩将其用作食品添加剂加入到冰红茶、枸杞、红酒、辣椒面、火锅底料中, 更有甚者将其添加到婴幼儿食用的糖果中, 以提高其表面色彩亮度 [3] . 目前已知的关于检测 RhB 的方法有高效液相色谱法 [4,5] 、电化学法 [6] 、荧光法 [7] 等, 然而, 上述方法存在样品前处理繁琐、检测时间长和仪器操作复杂等缺点. 因此, 目前迫切需要开发一种简单、可靠的方法对 RhB 进行快速超灵敏检测 [8] .…”

Section: 引言unclassified

Trace Detection of Rhodamine B in Infant Candy by g-C₃N₄/Ag Nanocomposite as Surface-Enhanced Raman Scattering Substrate

Ma¹,

Wu²,

Zhu³

et al. 2019

Acta Chim. Sinica

View full text Add to dashboard Cite

摘要近年来, 由婴幼儿食品中存在非法添加剂所引起的食品安全问题已经受到了广泛的关注. 表面增强拉曼散射 (SERS)技术可以对食品中被禁止添加的常用染料分子罗丹明 B (RhB)进行快速无损超灵敏的检测. 通过在类石墨相氮化碳(g-C 3 N 4)纳米片上组装 Ag 纳米粒子的方式构筑了 g-C 3 N 4 /Ag 复合材料, 并采用透射电子显微镜(TEM)、紫外可见近红外分光光度计(UV-Vis)、X 射线衍射仪(XRD)、荧光光谱仪和共聚焦显微拉曼光谱仪(Raman)对复合材料的形貌和结构进行了表征. g-C 3 N 4 纳米片不仅具有高度的离域 π 共轭体系和良好的吸附染料分子的性能, 而且可以作为 Ag 纳米粒子的载体, 使 Ag 纳米粒子均匀稳定地分散在其表面. 通过控制实验条件, 优化了测试过程中的 pH 及基底与 RhB 的结合时间, 详细探究 pH 对基底表面等离子共振的影响和对探针分子 SERS 强度的影响.

show abstract

Development of a rapid, simple and sensitive HPLC-FLD method for determination of rhodamine B in chili-containing products

Cited by 77 publications

References 5 publications

In Situ Quantitative Graphene-Based Surface-Enhanced Raman Spectroscopy

In Situ Quantitative Graphene-Based Surface-Enhanced Raman Spectroscopy

Nanosilica-based molecularly imprinted polymer nanoshell for specific recognition and determination of rhodamine B in red wine and beverages

Trace Detection of Rhodamine B in Infant Candy by g-C₃N₄/Ag Nanocomposite as Surface-Enhanced Raman Scattering Substrate

Contact Info

Product

Resources

About

Development of a rapid, simple and sensitive HPLC-FLD method for determination of rhodamine B in chili-containing products

Cited by 77 publications

References 5 publications

In Situ Quantitative Graphene-Based Surface-Enhanced Raman Spectroscopy

In Situ Quantitative Graphene-Based Surface-Enhanced Raman Spectroscopy

Nanosilica-based molecularly imprinted polymer nanoshell for specific recognition and determination of rhodamine B in red wine and beverages

Trace Detection of Rhodamine B in Infant Candy by g-C3N4/Ag Nanocomposite as Surface-Enhanced Raman Scattering Substrate

Contact Info

Product

Resources

About

Trace Detection of Rhodamine B in Infant Candy by g-C₃N₄/Ag Nanocomposite as Surface-Enhanced Raman Scattering Substrate