Development of a real-time enzymatic recombinase amplification assay (RT-ERA) and an ERA combined with lateral flow dipsticks (LFD) assay (ERA-LFD) for rapid detection of acute hepatopancreatic necrosis disease (AHPND) in shrimp Penaeus vannamei

Zhou, Qingqian; Wang, Yan; Hu, Jingjie; Bao, Zhenmin; Wang, Mengqiang

doi:10.1016/j.aquaculture.2022.739205

Aquaculture

2023

DOI: 10.1016/j.aquaculture.2022.739205

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Development of a real-time enzymatic recombinase amplification assay (RT-ERA) and an ERA combined with lateral flow dipsticks (LFD) assay (ERA-LFD) for rapid detection of acute hepatopancreatic necrosis disease (AHPND) in shrimp Penaeus vannamei

Qingqian Zhou

Yan Wang

Jingjie Hu

et al.

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Cited by 10 publications

References 41 publications

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Rapid detection of murine‐derived ingredients in meat products using real‐time enzymatic recombinase amplification

Xie,

Liu,

Liang

et al. 2024

J Sci Food Agric

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BACKGROUNDAccurate identification of meat species is critical to prevent economic fraud and safeguard public health. The use of inappropriate meat sources, such as murine, poses significant health risks because of potential contamination with pathogens and allergens, leading to foodborne illnesses. The present study aimed to develop a novel real‐time enzymatic recombinase amplification (ERA) method for the rapid and specific detection of murine DNA in meat products.RESULTSA novel ERA primer and probe set was designed, targeting a murine‐specific single‐copy nuclear gene identified through bioinformatics analysis. The assay demonstrates high specificity, showing no amplification in commonly consumed meats, other animals or major crops. Additionally, it exhibits remarkable sensitivity, detecting as few as five copies of murine genomic DNA. For practical application, the ERA method could effectively identify mouse DNA in laboratory‐prepared samples at concentrations as low as 0.5% and also quantify samples with mouse DNA content as low as 5%. It also accurately detects the presence of murine‐derived ingredients in commercially available meat products. The detection process is straightforward, utilizing a simple isothermal device for incubation, blue light excitation and a smartphone camera for result interpretation. This rapid analysis can be completed within 20 min.CONCLUSIONThe newly developed real‐time ERA method provides a valuable tool for standardizing meat trade practices, promoting food safety and enhancing consumer confidence in the authenticity of meat products. © 2024 Society of Chemical Industry.

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Rapid detection of murine‐derived ingredients in meat products using real‐time enzymatic recombinase amplification

Xie,

Liu,

Liang

et al. 2024

J Sci Food Agric

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A new highly sensitive lateral flow immunochromatographic assay for the detection of PirB toxin from acute hepatopancreatic necrosis disease-causing Vibrio species

Wangman,

Pengsuk,

Hajimasalaeh

et al. 2023

Aquacult Int

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Rapid and sensitive detection of Taura syndrome virus (TSV) in shrimp based on an isothermal enzymatic recombinase amplification (ERA) assay

Li,

Hu,

Bao

et al. 2024

Aquacult Int

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Development of a real-time enzymatic recombinase amplification assay (RT-ERA) and an ERA combined with lateral flow dipsticks (LFD) assay (ERA-LFD) for rapid detection of acute hepatopancreatic necrosis disease (AHPND) in shrimp Penaeus vannamei

Cited by 10 publications

References 41 publications

Rapid detection of murine‐derived ingredients in meat products using real‐time enzymatic recombinase amplification

Rapid detection of murine‐derived ingredients in meat products using real‐time enzymatic recombinase amplification

A new highly sensitive lateral flow immunochromatographic assay for the detection of PirB toxin from acute hepatopancreatic necrosis disease-causing Vibrio species

Rapid and sensitive detection of Taura syndrome virus (TSV) in shrimp based on an isothermal enzymatic recombinase amplification (ERA) assay

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