Development of a real‐time PCR method for the identification of wheat genotypes carrying different eyespot resistance genes

Meyer, Nina; Lind, V.; Karlovský, Petr; Zahn, Marc; Friedt, Wolfgang; Ordon, Frank

doi:10.1111/j.1439-0523.2010.01808.x

Cited by 6 publications

(8 citation statements)

References 41 publications

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“…The risk of incidence depends on many other factors such as sowing time, soil type, tillage methods, crop rotation (Bock et al, 2009;Jenkyn et al, 2010;Burnett et al, 2012). This disease is particularly significant in agricultural systems with reduced rotation, because both pathogens can infect perennial grasses of the Poaceae family and a wide range of cereals including rye (Meyer et al, 2011). Under favourable conditions, eyespot can damage spring cereals too.…”

Section: Introductionmentioning

confidence: 99%

Evaluation of eyespot incidence and structure of Oculimacula spp. population in winter rye in Lithuania

Ramanauskienė

Gaurilčikienė

Supronienė

et al. 2014

Zemdirbyste-Agriculture

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Eyespot of rye, caused by two fungal pathogens Oculimacula yallundae and O. acuformis, is widespread throughout many cool and wet cereal growing regions. The study was aimed to estimate eyespot incidence in winter rye crops in three agro-ecological zones of Lithuania, and to quantify eyespot disease causal agents O. yallundae and O. acuformis in the population using the real-time polymerase chain reaction (RT PCR) method. During the 2008-2012 periods, 69 winter rye fields were surveyed and eyespot-affected stems were identified in 92.7% of the crops surveyed. The incidence of eyespot in winter rye crops varied from 8.0% to 82.7% depending on the year and location. The average disease incidence in the reviewed crops was 26.0%. The highest incidence of eyespot averaging 34.0% was identified in the winter rye crops of the Middle Lowland zone. Both eyespot causal agents O. yallundae and O. acuformis were often found to co-exist on the winter rye stems. In the majority of the winter rye crops tested, O. acuformis was predominant in the Oculimacula spp. population. In most cases, O. yallundae was identified only at trace-level concentrations.

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Section: Introductionmentioning

confidence: 99%

Evaluation of eyespot incidence and structure of Oculimacula spp. population in winter rye in Lithuania

Ramanauskienė

Gaurilčikienė

Supronienė

et al. 2014

Zemdirbyste-Agriculture

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“…As there were only two data points for IT (susceptible and resistant) on RILs inoculated with Pca race LCBB, a correlation analysis could not be performed, but RILs with a resistant IT had significantly ( P < 0.001) lower mean relative fDNA content (2.29) than RILs with a susceptible IT (5.59). This study corroborates previous results that reported a significant correlation between fDNA and the severity of various diseases such as fusarium head blight in wheat (Burlakoti et al ., ), tan spot and glume blotch in wheat (Abdullah et al ., ), eyespot in wheat (Meyer et al ., ), and mycotoxin accumulation due to fusarium head blight in wheat (Burlakoti et al ., ).…”

Section: Discussionmentioning

confidence: 99%

“…For example, Meyer et al . () used a SYBR Green assay to detect Pch1 and Pch2 genes that are effective against eyespot in wheat and Acevedo et al . () used a TaqMan assay to map QTLs conferring partial resistance in oat to crown rust.…”

Section: Discussionmentioning

confidence: 99%

Development and validation of a quantitative PCR assay method of assessing relative resistance of oat (Avena sativa) to crown rust (Puccinia coronata f. sp. avenae)

et al. 2019

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Evaluation of oat crown rust resistance is usually based on visual assessment of disease severity or infection types. Visual assessment is subjective, prone to rater bias and requires expert knowledge. PCR-based quantitative assays can overcome challenges associated with visual assessment. New TaqMan primers and probes were designed from Puccinia coronata f. sp. avenae (Pca) sequences. The primer-probe sets were specific to Pca, amplified using as little as 0.5 pg fungal DNA (fDNA) and allowed for scaling to variation in sample total DNA quantity. The quantitative PCR (qPCR) assay was validated using oat recombinant inbred lines (RILs) from the Provena 9 94197A1-9-2-2-2-5 cross evaluated under a controlled environment. For comparison with fDNA load, inoculation with the Pca race LCBB provided segregation data on the hypersensitive response, while Pca race LSLG provided data on segregation for reduced pustule number. fDNA content was positively correlated with both pustule number and infection type (IT). Composite interval mapping identified two quantitative trait loci (QTLs) on oat linkage groups Mrg12 and Mrg20 using visual and qPCR assessments (pustule number, IT and fDNA). In this study a qPCR assay method that can be used to assess the relative resistance of oat to crown rust was refined and validated, and single nucleotide polymorphisms (SNPs) closely linked with two QTLs derived from the crown rust resistant line 94197A1-9-2-2-2-5 were identified.

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“…Tapesia yallundae), previously known as Pseudocercosporella herpotrichoïdes), remains problematic even in conventional agriculture and may decrease yield by up to 40% (Meyer et al, 2011). O.…”

Section: Introductionmentioning

confidence: 99%

Biocontrol of eyespot disease on two winter wheat cultivars by an anecic earthworm (Lumbricus terrestris)

Bertrand

Blouin

Barot

et al. 2015

Applied Soil Ecology

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trol of eyespot disease on two winter wheat cultivars by an anecic earthworm (Lumbricus terrestris). Applied Soil Ecology, Elsevier, 2015Elsevier, , 96, pp.33 -41. <10.1016Elsevier, /j.apsoil.2015 Eyespot is a major fungal disease of winter wheat, mostly affecting the base of the stem. The development of biological control approaches, using organisms such as earthworms, represents a potential alternative strategy for eyespot control. In a greenhouse experiment, we analyzed the response of two wheat cultivars (Soissons and Aubusson) to the presence of the pathogenic fungus Oculimacula yallundae and the anecic earthworm Lumbricus terrestris, alone and in combination. We assessed necrosis frequency, necrosis severity, wheat biomass, resource allocation and soil mineral concentrations. Disease incidence was lower in the presence of earthworms: the frequency of necrosis was 44% lower for Soissons and 70% lower for Aubusson. Necrosis severity was also lower for both cultivars (50% lower for Soissons and 80% lower for Aubusson) in the presence of earthworms. Earthworms had no detectable effect on the shoot and root biomasses of plants exposed to the fungus, but they modified resource allocation between plant organs and nutrient translocation within the plant. Our results suggest that earthworms are a potentially effective biocontrol agent for eyespot, and we discuss the possible underlying mechanisms.

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Development of a real‐time PCR method for the identification of wheat genotypes carrying different eyespot resistance genes

Cited by 6 publications

References 41 publications

Evaluation of eyespot incidence and structure of Oculimacula spp. population in winter rye in Lithuania

Evaluation of eyespot incidence and structure of Oculimacula spp. population in winter rye in Lithuania

Development and validation of a quantitative PCR assay method of assessing relative resistance of oat (Avena sativa) to crown rust (Puccinia coronata f. sp. avenae)

Biocontrol of eyespot disease on two winter wheat cultivars by an anecic earthworm (Lumbricus terrestris)

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