Development of a recombinant antigen‐based ELISA for the sero‐detection of porcine lymphotropic herpesviruses

Brema, Susanne; Lindner, Iris; Goltz, Michael; Ehlers, Bernhard

doi:10.1111/j.1399-3089.2008.00495.x

Cited by 23 publications

(25 citation statements)

References 20 publications

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“…Because the PCR assay detects only the viral DNA and does not provide evidence on viral replication, additional analysis based on complementary techniques, such as immunohistochemistry, in situ hybridization, and quantitative PCR should be conducted to confirm PLHV active infection and this previous hypothesis. Additionally, although the negative results from pigs at 4-18 days of age are in agreement with a previous study [23], we cannot rule out the possibility of the negative results be due to low PLHV genomic copies in the analyzed organs. Additional serological analysis also should be performed as a comparative assay to certainly assert the negative results, especially from pigs at this age group.…”

Section: Resultssupporting

confidence: 54%

Porcine lymphotropic herpesvirus DNA detection in multiple organs of pigs in Brazil

et al. 2020

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We investigated the porcine lymphotropic herpesvirus (PLHV) DNA presence in multiple organs of pigs. Biological samples (n = 136) included tissue fragments of the central nervous system, heart, kidney, liver, lungs, spleen, urinary bladder, and urine. Sixty-eight (50%) organs were PLHV DNA-positive. None of the urine samples were detected with the virus genome. Although the presence of the PLHV DNA in the urinary bladder and kidney has been detected, it was not possible to show whether urine can be considered an effective route of virus shedding. This study warns to the risk of PLHV zoonotic transmission by xenotransplantation of tissues of porcine origin.

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Section: Resultssupporting

confidence: 54%

Porcine lymphotropic herpesvirus DNA detection in multiple organs of pigs in Brazil

et al. 2020

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“…Screening for HEV‐ and PLHV‐specific antibodies was performed by Western blot analyses using 350 ng of a recombinant genotype 3 (GT3) open reading frame (ORF) 2–HEV antigen or 400 ng of recombinant glycoprotein B (gB1) of PLHV‐1 per lane. Recombinant gB1 protein was expressed as His‐tagged fusion protein in E. coli Rosetta (DE3) pLacI cells transformed with P1‐pTriEXgB1, encoding the N‐terminal part of the gB1 and purified using HisTrap columns (GE Healthcare, Buckinghamshire, Great Britain). Previous data demonstrated that the gB1 protein can be used to detect PLHV‐1‐, PLHV‐2‐, and PLHV‐3‐specific antibodies .…”

Section: Methodsmentioning

confidence: 99%

Microbiological characterization of a newly established pig breed, Aachen Minipigs

Plotzki

Heinrichs²,

Kubíčková

et al. 2016

Xenotransplantation

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“…Unless it is impossible to discriminate between viral latency and elimination of the virus by the immune response, antibody-positive animals should not be used as donors for xenotransplantation. Highly sensitive PCR-based and immunological methods have already been developed for PERVs ([for review see [22]), PCMV [25], [26], [27], [28], [29], HEV genotype 3 [30], [31], [32], [33], [34], [35], PCV2 [36], [37], [38] and PLHV1, 2, 3 [39], [40] in some specialised laboratories and there is hope that they will be used and improved in future in many test laboratories. As mentioned above, sensitive methods are the prerequisite for an effective detection of PCMV whereas detection methods of low sensitivity failed [20].…”

Section: Detection Methods and Elimination Programsmentioning

confidence: 99%

Xenotransplantation — A special case of One Health

Denner

2017

One Health

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The chronic shortage of human transplants to treat tissue and organ failure has led to the development of xenotransplantation, the transplantation of cells, tissues and organs from another species to human recipients. For a number of reasons, pigs are best suited as donor animals. Successful, routine xenotransplantation would have an enormous impact on the health of the human population, including the young, who sometimes require a replacement organ or islet cells, but especially the elderly, who more often suffer the consequences of organ failure. The first form of xenotransplantation applied to humans is the use of pig islet cells to treat insulin-dependent diabetes, a procedure that will have a significant economic impact. However, although xenotransplantation using pig cells, tissues and organs may save and prolong the lives of patients, it may also be associated with the transmission of porcine microorganisms to the recipient, eventually resulting in emerging infectious diseases. For this reason, the health of both the donor animals and the human recipients represents a special and sensitive case of the One Health concept. Basic research leading to strategies how to prevent transmission of porcine microorganisms by selection of virus-free animals, treatment of donor pigs by antiviral drugs, vaccines, colostrum deprivation, early weaning, Caesarean delivery, embryo transfer and/or gene editing should be undertaken to supply an increasing number of potential recipients with urgently required transplants. The methods developed for the detection and elimination of porcine microorganisms in the context of xenotransplantation will also contribute to an improvement in the health of pig populations in general and an increase in the quality of meat products. At present, there is evidence for transmission of porcine viruses to humans eating pork and having contact with pigs, however the impact of these viruses on public health is still unknown.

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Development of a recombinant antigen‐based ELISA for the sero‐detection of porcine lymphotropic herpesviruses

Cited by 23 publications

References 20 publications

Porcine lymphotropic herpesvirus DNA detection in multiple organs of pigs in Brazil

Porcine lymphotropic herpesvirus DNA detection in multiple organs of pigs in Brazil

Microbiological characterization of a newly established pig breed, Aachen Minipigs

Xenotransplantation — A special case of One Health

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