Development of a recombinant OppA-based indirect hemagglutination test for the detection of antibodies against Haemophilus parasuis

Chen, Shengli; Chu, Yuefeng; Zhao, Ping; He, Yiliang; Jian, Yingna; Liu, Yongsheng; Lu, Zengjun

doi:10.1016/j.actatropica.2015.04.009

Cited by 9 publications

(8 citation statements)

References 24 publications

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“…However, no recent seroepidemiology reports were accessible for the Sichuan province and the prevalent serovars may change over time. The serovar profile in the present investigation was partly consistent with previous reports in China mainland, however, following serovars 4 and 5 were serovars 7, 1 and 2 rather than serovars 14, 13 and 12 as previously reported (Cai et al, 2005; Zhang et al, 2012; Chen et al, 2015). Furthermore, despite the most two epidemic serovars being the same in the two parts of the Sichuan province, the secondary serovars may interfere in the control of H. parasuis infection.…”

Section: Discussionsupporting

confidence: 93%

Prevalence and seroepidemiology ofHaemophilus parasuisin Sichuan province, China

Wang

Zhao

Wei

et al. 2017

PeerJ

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Haemophilus parasuis, the causative agent of Glässer’s disease, has been reported as widespread, but little is known about its epidemiology in the Sichuan province of China. The goal of our research is to reveal the prevalence and distribution of H. parasuis in this area. Sampling and isolation were performed across Sichuan; isolates were processed using serotyping multiplex PCR (serotyping-mPCR) and agar gel diffusion (AGD) for confirmation of serovar identity. This study was carried out from January 2014 to May 2016 and 254 H. parasuis field strains were isolated from 576 clinical samples collected from pigs displaying clinical symptoms. The isolation frequency was 44.10%. Statistically very significant differences of infection incidence were found in three age groups (P < 0.01) and different seasons (P < 0.01). Serovars 5 (25.98%) and 4 (23.62%) were the most prevalent, however, non-typeable isolates accounted for nearly 7.87%. In terms of geographical distribution, serovars 5 and 4 were mostly prevalent in west and east Sichuan. The results confirmed that the combined approach was dependable and revealed the diversity and distribution of serovars in Sichuan province, which is vital for efforts aimed at developing vaccine candidates allowing for the prevention or control of H. parasuis outbreaks.

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Section: Discussionsupporting

confidence: 93%

Prevalence and seroepidemiology ofHaemophilus parasuisin Sichuan province, China

Wang

Zhao

Wei

et al. 2017

PeerJ

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“…In southern China in 2011, the serotypes 4, 5, 13, 15, and 2 predominated ( Zhang et al, 2011 ). Other three studies in China indicated the documented serotypes were 4, 5, 14, 13 in 2015 and 5, 4, 7, 13 in 2016 and 4, 5, 7, 1 in 2017 ( Chen et al, 2015 ; Ma et al, 2016 ; Wang et al, 2017 ). In addition to these studies, high NT isolation rates (10–40%) were reported in all previously described studies, which maybe become a dominant serotype in Germany, USA/Canada, Denmark and Brazil ( Angen, Svensmark & Mittal, 2004 ; Kielstein & Rapp-Gabrielson, 1992 ; Rapp-Gabrielson & Gabrielson, 1992 ), and even became the most serotype in Brazil ( Macedo et al, 2011 ; Moreno et al, 2011 ).…”

Section: Discussionmentioning

confidence: 96%

Epidemiology ofHaemophilus parasuisisolates from pigs in China using serotyping, antimicrobial susceptibility, biofilm formation and ERIC-PCR genotyping

Zhao

Lin

et al. 2018

PeerJ

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BackgroundHaemophilus parasuis is a commensal organism of the upper respiratory tract of healthy pigs and causes high morbidity and mortality in piglets. The aim of this study was to investigate the epidemiology of H. parasuis in China from 2014 to 2017.MethodsWe characterized 143 H. parasuis isolates by serotyping, antimicrobial susceptibility, biofilm formation and with enterobacterial repetitive intergenic consensus-polymerase chain reaction (ERIC-PCR) assays.ResultsSerotyping revealed serovar 5 as the most prevalent (26.6%) followed by serovars 4 (22.4%), 7 (9.1 %), 13 (6.3%), 12 (5.6 %), and non-typeable (8.4%). In a panel of 23 antimicrobials, the minimum inhibitory concentration 50% (MIC50) were in the range of 0.25–16 μg/mL and MIC90 were 2–>512 μg/mL. A total of 99 isolates of H. parasuis (69.2%) were able to form biofilms and 59.6% (59/99) performed weak biofilm-forming ability. ERIC-PCR revealed a very heterogeneous pattern with 87 clusters.DiscussionThese H. parasuis isolates showed a high serovar and genotypic lineage diversity, different abilities to form biofilms and a high degree of genetic diversity. Biofilm formation was related to antimicrobial susceptibility but there were no statistically significant associations between the antimicrobial susceptibility and either the serovars or the ERIC-PCR clusters. This study showed a high prevalence of high-MIC H. parasuis strains and suggests the need for a continuous surveillance of clinical isolates of H. parasuis.

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“…Recently, the researchers used recombinant OppA as the antigen of an indirect hemagglutination assay for detecting antibodies against H . parasuis [38]. The TonB-dependent haem receptor HxuC belongs to the hxuCBA gene cluster, which is a virulence factor of H .…”

Section: Discussionmentioning

confidence: 99%

Evaluation of immunogenicity and protective efficacy of recombinant outer membrane proteins of Haemophilus parasuis serovar 5 in a murine model

Cai

Song

et al. 2017

PLoS ONE

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Glässer’s disease is an economically important infectious disease of pigs caused by Haemophilus parasuis. Few vaccines are currently available that could provide effective cross-protection against various serovars of H. parasuis. In this study, five OMPs (OppA, TolC, HxuC, LppC, and HAPS_0926) identified by bioinformatic approaches, were cloned and expressed as recombinant proteins. Antigenicity of the purified proteins was verified through Western blotting, and primary screening for protective potential was evaluated in vivo. Recombinant TolC (rTolC), rLppC, and rHAPS_0926 proteins showing marked protection of mice against H. parasuis infection, and were further evaluated individually or in combination. Mice treated with these three OMPs produced humoral and host cell-mediated responses, with a significant rise in antigen-specific IgG titer and lymphoproliferative response in contrast with the mock-immunized group. Significant increases were noted in CD4+, CD8+ T cells, and three cytokines (IL-2, IL-4, and IFN-γ) in vaccinated animals. The antisera against candidate antigens could efficiently impede bacterial survival in whole blood bactericidal assay against H. parasuis infection. The multi-protein vaccine induced more pronounced immune responses and offered better protection than individual vaccines. Our findings indicate that these three OMPs are promising antigens for the development of multi-component subunit vaccines against Glässer's disease.

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Development of a recombinant OppA-based indirect hemagglutination test for the detection of antibodies against Haemophilus parasuis

Cited by 9 publications

References 24 publications

Prevalence and seroepidemiology ofHaemophilus parasuisin Sichuan province, China

Prevalence and seroepidemiology ofHaemophilus parasuisin Sichuan province, China

Epidemiology ofHaemophilus parasuisisolates from pigs in China using serotyping, antimicrobial susceptibility, biofilm formation and ERIC-PCR genotyping

Evaluation of immunogenicity and protective efficacy of recombinant outer membrane proteins of Haemophilus parasuis serovar 5 in a murine model

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