Development of a repeated exposure protocol of human bronchial epithelium in vitro to study the long-term effects of atmospheric particles

Boublil, Laura; Assémat, Emeline; Borot, Marie-Caroline; Boland, Sonja; Martinon, Laurent; Sciare, Jean; Baeza‐Squiban, Armelle

doi:10.1016/j.tiv.2012.11.008

Cited by 51 publications

(25 citation statements)

References 44 publications

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“…Figure 1 continues with part of the results we previously showed [12] and, is shown to remind us of the characteristics of the conditioned culture media we used in the present study. Treatments were performed on undifferentiated HBE cells just after they started to be grown at the air-liquid interface (ALI).…”

Section: Effects Of Repeated Exposure To Fine Particles On the Releassupporting

confidence: 77%

“…Our previous studies performed on bronchial epithelial cell lines demonstrated a higher pro-inflammatory effect of fine and ultrafine particles compared to coarse ones for particles coming from rural sites, traffic or background urban sites in France as well as African cities [14][15][16]. This was also observed for HBE cells repeatedly exposed to fine, ultrafine and coarse PM [12]. In order to determine whether such differences could also be observed on proliferation of fibroblasts, we compared the effects of conditioned media recovered during repeated treatments of HBE cells to either coarse, fine or ultrafine PM at 5 µg/cm².…”

Section: Effect Of Different Particle Size On Fibroblast Proliferationmentioning

confidence: 63%

“…Once cells had reached confluency, the air-liquid interface (ALI) was established by removing the apical medium and retinoic acid (10 -7 M) was added to the basal medium that was changed every two days. Four treatments (spaced by 48 hours: T1, T2, T3 and T4) of four hours (at 5 and 10 µg/cm 2 ) were applied on NHBE cells [12]. Basal media were collected 48 hours after each treatment as well as in the following weeks after the end of the treatments in order to measure growth factor release and to be used as conditioned media for fibroblasts.…”

Section: Nhbe Cell Culture and Exposurementioning

confidence: 99%

“…Contributing to airway remodeling, chronic exposure to PM could favor airflow obstruction in compromised people living in cities with high levels of PM. We took advantage of an experimental model, the primary culture of normal bronchial epithelial (HBE) cells grown at the air-liquid interface (ALI), which we developed for repeated exposures to PM [12], to investigate the effects of the epithelial secretome on fibroblasts. For this purpose two different approaches were used.…”

Section: Introductionmentioning

confidence: 99%

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The Secretome of Human Bronchial Epithelial Cells Exposed to Fine Atmospheric Particles Induces Fibroblast Proliferation

Boublil

Martinon²,

Baeza‐Squiban

2013

Challenges

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Chronic exposure to particulate pollution is suspected to exacerbate inflammatory respiratory diseases such as asthma characterized by an airway remodelling involving fibrosis. Our study aims to investigate whether the secretome from human bronchial epithelial (HBE) cells exposed to fine particulate matter (PM) induces fibroblast proliferation. Primary HBE cells grown on air liquid interface were repeatedly exposed to fine PM at 5 and 10 µg/cm² (four treatments, 48 hours apart) and maintained in culture for five weeks. Collected basolateral culture medium was used as a conditioned medium for the subsequent treatment of fibroblasts. We observed that the conditioned medium collected from HBE cells treated with fine PM increased the growth rate of fibroblasts compared to the conditioned medium collected from control HBE cells. Fibroblast phenotype assessed by the observation of the vimentin network was well preserved. The mitogenic effect of conditioned medium was reduced in the presence of anti-epidermal growth factor receptor (EGFR), anti-amphiregulin or anti-TGFa, underlining the role of EGFR ligands in fibroblast proliferation. When fibroblasts were co-cultured with HBE cells treated once with fine PM, they exhibited a higher growth rate than fibroblasts co-cultured with non-treated HBE cells. Altogether these data show that the exposure of HBE cells to fine PM induced the production of EGFR ligands in sufficient amount to stimulate fibroblast proliferation providing insight into the role of PM in airway remodelling

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Section: Effects Of Repeated Exposure To Fine Particles On the Releassupporting

confidence: 77%

Section: Effect Of Different Particle Size On Fibroblast Proliferationmentioning

confidence: 63%

Section: Nhbe Cell Culture and Exposurementioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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The Secretome of Human Bronchial Epithelial Cells Exposed to Fine Atmospheric Particles Induces Fibroblast Proliferation

Boublil

Martinon²,

Baeza‐Squiban

2013

Challenges

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“…When HBEC, derived from immortalized cell lines or normal donors, are exposed to ambient PM or DEPs, a strong up‐regulation of different epithelial cytokines can be observed. The levels of IL‐6, IL‐8 and TNF‐α, for instance, were significantly increased in cells that were exposed to PM. Of note, also MIP‐3α/CCL20, an important chemokine involved in DC recruitment, was increased in HBEC upon ambient PM exposure.…”

Section: Air Pollution and Asthmamentioning

confidence: 99%

Insights in particulate matter‐induced allergic airway inflammation: Focus on the epithelium

Grove

Provoost

Brusselle

et al. 2018

Clin Experimental Allergy

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Outdoor air pollution is a major environmental health problem throughout the world. In particular, exposure to particulate matter (PM) has been associated with the development and exacerbation of several respiratory diseases, including asthma. Although the adverse health effects of PM have been demonstrated for many years, the underlying mechanisms have not been fully identified. In this review, we focus on the role of the lung epithelium and specifically highlight multiple cytokines in PM-induced respiratory responses. We describe the available literature on the topic including in vitro studies, findings in humans (ie observations in human cohorts, human controlled exposure and ex vivo studies) and in vivo animal studies. In brief, it has been shown that exposure to PM modulates the airway epithelium and promotes the production of several cytokines, including IL-1, IL-6, IL-8, IL-25, IL-33, TNF-α, TSLP and GM-CSF. Further, we propose that PM-induced type 2-promoting cytokines are important mediators in the acute and aggravating effects of PM on airway inflammation. Targeting these cytokines could therefore be a new approach in the treatment of asthma.

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Reality Check for Nanomaterial‐Mediated Therapy with 3D Biomimetic Culture Systems

Tay

Muthu

Chia

et al. 2016

Adv Funct Materials

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The recent progresses in tissue engineering and nanomaterial‐based therapeutics/theranostics have led to the ever increasing utilization of 3D in vitro experimental models as the bona fide culture systems to evaluate the therapeutic/theranostic effects of nanomedicine. Compared to the use of conventional 2D culture platforms, 3D biomimetic cultures offer unmatched advantages as relevant physiological and pathological elements can be incorporated to allow better characterization of the engineered bio‐nanomaterials in the targeted tissue‐specific microenvironment. In this Feature Article, the current state‐of‐the‐art 3D in vitro models that have been developed for the evaluation of biosafety and efficacy of nano‐ therapeutics/theranostics targeting the colon, blood–brain barrier (BBB), lungs, skin tumor models to bridge the nanomedicine bench to pre‐clinical ravine are reviewed. Furthermore, the critical physicochemical parameters of the bio‐nanomaterials that govern its transport and biodistribution in a complex 3D microenvironment will be highlighted. The major challenges and future prospects of evaluating nanomedicine in the third dimension will also be discussed.

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Development of a repeated exposure protocol of human bronchial epithelium in vitro to study the long-term effects of atmospheric particles

Cited by 51 publications

References 44 publications

The Secretome of Human Bronchial Epithelial Cells Exposed to Fine Atmospheric Particles Induces Fibroblast Proliferation

The Secretome of Human Bronchial Epithelial Cells Exposed to Fine Atmospheric Particles Induces Fibroblast Proliferation

Insights in particulate matter‐induced allergic airway inflammation: Focus on the epithelium

Reality Check for Nanomaterial‐Mediated Therapy with 3D Biomimetic Culture Systems

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