Development of a robust and standardized immunoserological assay for detection of anti-measles IgG antibodies in human sera

Böröcz, Katalin; Csizmadia, Zsuzsanna; Mészáros, Viktória; Farkas, Nelli; Telek, Vivien; Varga, Vivien; Maloba, Geofrey Ouma; Bodó, Kornélia; Najbauer, Joseph; Berki, Tímea; Németh, Pèter

doi:10.1016/j.jim.2018.07.009

Cited by 8 publications

(29 citation statements)

References 28 publications

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“…Anti-measles antibody (IgG)-level data were considered an adequate model for pathogen-derived (or infection-induced) antibodies that are present in the great majority of the Hungarian population. Based on our previous findings [20,21] and in accordance with data of other Hungarian colleagues [22,23], the overall ratio of the Hungarian population in possession of sufficient anti-measles IgG antibody levels is ≈90%, with a well-characterized, more susceptible cluster being responsible for the lagging ≈10%. Age group categorization based on historical changes in the measles, mumps and rubella (MMR) immunization schedule, recurring epidemics, subsequent revaccination protocols and shifts between vaccine valency (mono-, bi-or trivalent) and manufacturers have been thoroughly detailed previously [20,21,24].…”

Section: Anti-measles Igg Elisasupporting

confidence: 88%

Relationship between natural and infection-induced antibodies in systemic autoimmune diseases (SAD): SLE, SSc and RA

Böröcz

Simon

Erdő-Bonyár

et al. 2020

Clinical and Experimental Immunology

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Our goal was to explore immunoserological relations between IgM and IgG isotypes of natural autoantibodies (nAAbs) and pathogen (or vaccine)‐induced or disease‐related antibodies in systemic autoimmune diseases (SAD); systemic sclerosis (SSc), systemic lupus erythematosus (SLE), rheumatoid arthritis (RA). The found significant associations between IgG isotype nAbs and specific humoral antibodies may underscore the immune response‐inducible nature of the diseases investigated, and may corroborate the notion that nAbs can act as a mediator between the innate‐like and the adaptive arms of the immune system .The relationship between protective anti‐dsDNA IgM and the IgM isotype of anti‐F4 and anti‐CS may provide immuno‐serological evidence for the beneficial roles of nAAbs in SLE patients.

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Section: Anti-measles Igg Elisasupporting

confidence: 88%

Relationship between natural and infection-induced antibodies in systemic autoimmune diseases (SAD): SLE, SSc and RA

Böröcz

Simon

Erdő-Bonyár

et al. 2020

Clinical and Experimental Immunology

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“…The samples are considered representative, as clinical residual samples were randomly selected (with the exclusion of seriously immunocompromised patients) from the Department of Laboratory Medicine, University of Pécs, Medical School, which serves three counties (Baranya, Somogy and Tolna, with a population of ∼8 87 000), and receives laboratory examination requests from all over Hungary. In case of measles serosurvey, in order to give a more accurate estimate at population level, we combined our recently published data with the results of current measurements (previously we tested 1985 serum samples for measles [24], of which the data of 1787 samples have been pooled together with the current data; 'cumulative' data for measles, N total measles = 3523). Serum samples were from all age groups (beginning from the era before the implementation of measles vaccine, through several different vaccine types, manufacturers and vaccination schedules, up to present), and were categorised based on past changes introduced in measles and MMR immunisation schedules ( Table 1).…”

Section: Samplesmentioning

confidence: 99%

“…Testing of blocking reagents was performed using bovine gelatine, milk powder, Block ACE (Bio-Rad) and our in-house polyvinyl alcohol (PVA)-based purely synthetic blocking buffer. Details of sample pre-treatment and assay preparation steps have been described earlier [24]. To demonstrate the lack of interference when using cell culturederived antigen coatings, we compared our 'target' assay to purified recombinant viral capsid protein antigen-based assays.…”

Section: Antigen Coatingmentioning

confidence: 99%

“…From 1 July 2018 to 30 June 2019, EU/EEA Member States reported 483 cases of rubella. The highest number of cases was reported by Poland (372), Germany (57), Italy (24), Spain (12) and Romania (4) [5]. For Hungary, between 2007 and 2018, WHO reports only 10 cases [12].…”

Section: Introductionmentioning

confidence: 99%

“…To our knowledge, this triple format of MMR ELISA is currently not available on the market. The ELISA protocol described herein incorporates our previous method [24] that has been further improved to enable the use of the same assay conditions for all three anti-viral antibodies. We demonstrate the high-throughput applicability of this assay using 1736 serum samples from patients of diverse age groups, and provide an estimation of the population-level MMR seropositivity.…”

Section: Introductionmentioning

confidence: 99%

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Application of a fast and cost-effective ‘three-in-one’ MMR ELISA as a tool for surveying anti-MMR humoral immunity: the Hungarian experience

et al. 2020

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In Hungary, between February 2017 and July 2019, 70 confirmed measles cases were reported, raising questions about the adequacy of population-level immunity. Although the assumed vaccination coverage is ≥99%, in a recent study, we detected potential gaps in the anti-measles humoral immunity. In Hungary, according to a decree by the Ministry of Public Welfare, beginning from 2021, the healthcare provider should conduct a serosurvey of anti-measles protection levels of healthcare professionals. To facilitate the compliance with this requirement, we developed a quick ‘three-in-one’ or ‘triple’ MMR (measles, mumps and rubella) indirect ELISA (IgG); an assay format that is currently not available commercially. High throughput applicability of the ‘three-in-one’ ELISA was verified using 1736 sera from routine laboratory residual samples, using an automated platform (Siemens BEP 2000 Advance). Assay verification was performed by comparing the full antigen repertoire-based ‘target’ assay with in-house ‘control’ assays using recombinant viral antigen coatings, and by validated commercially available kits. Indirect immunofluorescence was used as an independent reference method. Data were analysed using OriginLab, IBM SPSS, RStudio and MedCalc. In case of measles, we combined our current results with previously published data (Ntotal measles = 3523). Evaluation of anti-mumps and anti-rubella humoral antibody levels was based on the measurement of 1736 samples. The lowest anti-measles seropositivity (79.3%) was detected in sera of individuals vaccinated between 1978 and 1987. Considering the antigen-specific seropositivity ratios of all samples measured, anti-measles, -mumps and -rubella IgG antibody titres were adequate in 89.84%, 91.82% and 92.28%, respectively. Based on the virus-specific herd immunity threshold (HIT) values (HITMeasles = 92–95%, HITMumps = 75–86%, HITRubella = 83–86), it can be stated that regarding anti-measles immunity, certain age clusters of the population may have inadequate levels of humoral immunity. Despite the potential gaps in herd immunity, the use of MMR vaccine remains an effective and low-cost approach for the prevention of measles, mumps and rubella infections.

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Az európai és a magyarországi kanyaróhelyzet összefoglalása és tanulságai

Lengyel¹,

Ánosi²,

Marossy³

et al. 2019

Orvosi Hetilap

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Measles is one of the most serious preventable infectious diseases, which in our country were among the rare diseases in the last 10 to 20 years. One of the reasons for this is that the Hungarian population born after 1969 was vaccinated in almost 99 percent. The other reason is that in the period prior to vaccination era, the often-occurring measles epidemics left life-long immunity in the affected persons. Thus, natural and artificial immunizations provided extensive herd immunity. However, the ongoing measles epidemics in Europe have highlighted the fact that the symptoms and differential diagnosis related to measles have been relegated to the negligible category for the last 20 years. In addition to reviewing the consequences of the European measles pandemics in Hungary, the purpose of this paper is to revise and summarize the clinical and laboratory knowledge required to establish a definitive epidemiological control of measles. Orv Hetil. 2019; 160(20): 767–773.

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Development of a robust and standardized immunoserological assay for detection of anti-measles IgG antibodies in human sera

Cited by 8 publications

References 28 publications

Relationship between natural and infection-induced antibodies in systemic autoimmune diseases (SAD): SLE, SSc and RA

Relationship between natural and infection-induced antibodies in systemic autoimmune diseases (SAD): SLE, SSc and RA

Application of a fast and cost-effective ‘three-in-one’ MMR ELISA as a tool for surveying anti-MMR humoral immunity: the Hungarian experience

Az európai és a magyarországi kanyaróhelyzet összefoglalása és tanulságai

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