Development of a robust blood smear preparation procedure for external quality assessment

Vu, Huy Quang; Van, Hy Triet; Tran, Van Thanh; Huynh, Thi Diem Phuc; Nguyen, Van Tinh; Le, Dinh Thanh

doi:10.1016/j.plabm.2021.e00253

Cited by 4 publications

(1 citation statement)

References 9 publications

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“…EDTA-blood was examined for the routine hematological profile using a fully automated hematology analyzer (Sysmex ® XP-100). Blood samples were also processed for regular thin blood smear preparation following Vu et al (2021) for morphological observation and confirmation with results generated by the machine.…”

Section: Data Samplingmentioning

confidence: 99%

Hemotoxicity of Hairy Fig (Ficus hispida L.f.) Fruits on Male Wistar Rats (Rattus norvegicus Berkenhout, 1769)

Fitria

Sari²,

Handayani

et al. 2022

Jurnal Biodjati

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Hairy fig (Ficus hispida) fruits (HFF) are widely consumed as food and traditional medicine in several West Asian countries, both the unripe fruit (UHFF) and ripe fruit (RHFF). However, they are not commonly utilized in Indonesia. Acute oral toxicity studies reported No Observed adverse effect level (NOAEL). Further, a reproductive toxicity study found that UHFF boosted spermatogenesis and increased the quality and quantity of spermatozoa. Meanwhile, RHFF exhibited the opposite effects. To provide comprehensive information from the previous study, this research was conducted to evaluate the hemotoxicity of UHFF and RHFF about their impact on the male reproductive system. Nine Wistar rats were assigned into three groups: the first group received UHFF juice, the second group received RHFF juice, and the third group received distilled water as control. The volume of each treatment was 2 mL/individual/day for 77 days. On days 0, 28, and 77, blood samples were collected for routine hematological profile examination using a hematology analyzer (Sysmex®XP-100). Data were analyzed using a one-way ANOVA test and Duncan's test (α=0.05) to discover significant differences between groups and times. Results showed that consuming hairy fig fruit, especially the UHFF, had an unfavorable effect on erythrocytes resulting in hypochromic microcytic anemia. Still, there was no adverse effect on leukocytes and platelets. Anemia may have occurred due to the presence of hemotoxic compounds that interfere with the synthesis and binding of hemoglobin or because the hairy fig fruit filtrates were oxidized, thereby increasing the level of oxidative stress within the body, of which is a drop in hemoglobin levels.

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Section: Data Samplingmentioning

confidence: 99%

Hemotoxicity of Hairy Fig (Ficus hispida L.f.) Fruits on Male Wistar Rats (Rattus norvegicus Berkenhout, 1769)

Fitria

Sari²,

Handayani

et al. 2022

Jurnal Biodjati

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Novel insights into antioxidant status, gene expression, and immunohistochemistry in an animal model infected with camel-derived Trypanosoma evansi and Theileria annulata

Ramadan,

Bakr,

Fouad

et al. 2024

Parasites Vectors

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Background Hemoprotozoan diseases, especially trypanosomosis and theileriosis, adversely affect the productivity, growth, and performance of camels. Regular sampling and investigation of camels are challenging due to several factors. Consequently, there is a lack of knowledge on camel parasite genotyping, cytokine production, and oxidative stress parameters during infection. Methods The present study investigated two critical blood protozoa infecting camels in Egypt, Trypanosoma evansi and Theileria annulata, using molecular methods, specifically 18S rRNA gene analysis. Following molecular confirmation, experimental infections were induced in Swiss albino mice to assess the expression of immune response genes and oxidative stress parameters. The study further explored the correlation between histopathological alterations and inflammatory reactions in the kidney, spleen, and liver of infected mice, alongside the immunohistochemical expression of caspase-3, proliferating cell nuclear antigen (PCNA), and tumor necrosis factor (TNF). Results Trypanosoma evansi and T. annulata isolated from naturally infected camels were molecularly identified and deposited in GenBank under accession numbers OR116429 and OR103130, respectively. Infection with T. evansi and T. annulata caused significant adverse effects on the immune condition of infected mice, increasing the pathogenicity of the infection. This was evidenced by a significant increase in oxidative stress parameter levels in both naturally infected camels and experimentally infected mice compared to healthy controls. Furthermore, the expression of immune response genes was significantly elevated in infected mice. Immunohistochemistry analysis showed a pronounced upregulation of caspase-3, PCNA, and TNF in the infected groups relative to the control group. These findings are the first to be reported in Egypt. Conclusions This study successfully identified and genotyped two economically important blood protozoa, T. evansi and T. annulata, from camels in Egypt. Additionally, the experimental animal model provided valuable insights into the immune response, oxidative stress, and histopathological changes induced by these parasites, demonstrating comparable results to naturally infected camels. These findings highlight the potential of this model to study parasite–host interactions and immune responses, contributing to a better understanding of the pathogenic mechanisms of T. evansi and T. annulata infections. This model may be useful for future studies focused on disease control and therapeutic interventions. Graphical Abstract

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Experimental assessment of the effective-medium approach for disordered monolayers of particles with high scattering losses

Acevedo-Barrera,

Méndez-Fragoso,

García-Valenzuela

2024

Opt. Lett.

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The validity of using an effective-medium approach (EMA) to model the reflectivity of a disordered monolayer of particles that scatter light significantly is tested experimentally. To achieve this, we measured the optical reflectivity versus the angle of incidence in an internal reflection configuration of a disordered monolayer of polymeric particles with negligible optical absorption and a diameter of about half a wavelength (size parameter of 1.2) deposited on a glass-air interface. We found a clear effective-medium film equivalence, even for low particle densities and for angles of incidence well beyond the critical angle, where light penetrates the monolayer less than a particle diameter.

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Development of a robust blood smear preparation procedure for external quality assessment

Cited by 4 publications

References 9 publications

Hemotoxicity of Hairy Fig (Ficus hispida L.f.) Fruits on Male Wistar Rats (Rattus norvegicus Berkenhout, 1769)

Hemotoxicity of Hairy Fig (Ficus hispida L.f.) Fruits on Male Wistar Rats (Rattus norvegicus Berkenhout, 1769)

Novel insights into antioxidant status, gene expression, and immunohistochemistry in an animal model infected with camel-derived Trypanosoma evansi and Theileria annulata

Experimental assessment of the effective-medium approach for disordered monolayers of particles with high scattering losses

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