2009
DOI: 10.1016/j.jaci.2009.04.007
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Development of a routine newborn screening protocol for severe combined immunodeficiency

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Cited by 168 publications
(138 citation statements)
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“…The first step was to test different TREC primers. TREC2F/2R [20] provide more robust results with DBS DNA than the original primers used [18], which produced more nonspecific bands in our system. The reference gene we chose was RNase P, which we assayed using primers designed by the CDC.…”
Section: Methods Development In Ntuhmentioning
confidence: 94%
See 1 more Smart Citation
“…The first step was to test different TREC primers. TREC2F/2R [20] provide more robust results with DBS DNA than the original primers used [18], which produced more nonspecific bands in our system. The reference gene we chose was RNase P, which we assayed using primers designed by the CDC.…”
Section: Methods Development In Ntuhmentioning
confidence: 94%
“…The T-cell receptor excision circles (TRECs) assay is a verified tool used for SCID screening in newborns [18,19]. However, there are many versions of primers and probes [19,20] and different combinations of reaction conditions used for the assay [1].…”
Section: Methods Development In Ntuhmentioning
confidence: 99%
“…21 TREC levels were normalized per microliter of blood, assuming that the sample contains approximately 3 mL of whole blood. Calibration curves were generated by means of 10-fold serial dilution of plasmids that contain TREC and b-actin sequences.…”
Section: Trec Analysismentioning
confidence: 99%
“…Soon thereafter, the MCW group in collaboration with the Wisconsin State Hygiene Laboratory optimized the TREC assay for NBS for SCID [23]. Changes in DNA extraction and improvements in the efficiency of the RT-qPCR amplification efficiency lowered the false positive rate to roughly 0.02%, as only one of the 5766 NBS cards tested failed to amplify TRECs and β-actin.…”
Section: Improvements In the Trec Assay Lead To Nbs For Scidmentioning
confidence: 99%