Development of a salmon growth hormone radioimmunoassay

Wagner, Graham F.; McKeown, B.A.

doi:10.1016/0016-6480(86)90055-9

Cited by 70 publications

(33 citation statements)

References 33 publications

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“…In both cases a highly significant elevation was observed in starved fish, the magnitude of which was similar to our results (nine and sevenfold in their two studies; sixfold in both of our experiments). However, although the results of McKeown superficially appear similar to ours, there is a major difference in that the growth hormone levels in fed fish in our experiments were around 1 ng ml ', whereas McKeown reported levels of 85.5 ng ml -r (Wagner and McKeown, 1986) and 6.4 ng ml-' (Barrett and McKeown, 1988) in fed trout. Two possible reasons for this difference in "basal" growth hormone levels in trout in good condition are discussed in detail in an accompanying paper (Le Bail et al, 1991).…”

Section: Discussionsupporting

confidence: 60%

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The effect of starvation on growth and plasma growth hormone concentrations of rainbow trout, Oncorhynchus mykiss

Sumpter

Bail

Pickering

et al. 1991

General and Comparative Endocrinology

169

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Two experiments, one using 0+ the other 1 + rainbow trout, were conducted to investigate the effect of prolonged starvation on plasma growth hormone levels. The results from both experiments were essentially the same. As expected, starvation resulted in cessation of growth and in a lower coefficient of condition, whereas fed fish continued to grow and remained in good condition. Starvation had relatively little effect on the plasma cortisol level; in one experiment levels were elevated temporarily in starved fish, although by the end of the experiment there was no longer any difference between starved and fed fish, and in the other experiment plasma cortisol levels remained very low throughout the course of the experiment in both starved and fed fish. In contrast, in both experiments starvation had a pronounced effect on the plasma growth hormone level, which rose steadily during both experiments, such that it was six times higher after 1 month of starvation in 0+ fish. and five times higher after 6 weeks of starvation in 1 + fish. Thus, paradoxically, fed fish had very low plasma growth hormone levels and grew rapidly, whereas starved fish had elevated plasma growth hormone levels but did not grow. In both experiments a strong negative correlation was observed between the plasma growth hormone level and the coefficient of condition of the fish. The results are discussed with regard to the well-established metabolic changes that occur during starvation, and it is suggested that a major role of growth hormone during starvation is to aid in the mobilisation of fatty acids and glycerol from adipose stores.

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Section: Discussionsupporting

confidence: 60%

“…McKeown and colleagues have reported the results of two experiments designed to assess the effects of starvation on plasma growth hormone levels in trout (Wagner and McKeown, 1986;Barrett and McKeown. 1988).…”

Section: Discussionmentioning

confidence: 99%

The effect of starvation on growth and plasma growth hormone concentrations of rainbow trout, Oncorhynchus mykiss

Sumpter

Bail

Pickering

et al. 1991

General and Comparative Endocrinology

169

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“…This contrasts with the observations of Cook and Peter (1984) who concluded that the stress of injection caused an increase in serum GH at 24 hr poststress in the goldfish. Carussius aura&s, and with those of Wagner and McKeown (1986) who were unable to find any effects of handling stress on circulating GH levels in rainbow trout. Acute stress can result in an increase in blood GH levels in primates (Muller, 1975) but suppresses GH release in rats (Terry et al, 1977).…”

Section: Discussionmentioning

confidence: 80%

“…The recent development and validation of teleost GH radioimmunoassays (Cook et al, 1983;Bolton et al, 1986;Wagner and McKeown, 1986;Le Bail et al, 1991) now opens up this field of stress physiology in teleost fish to practical investigation. The present study was designed to investigate the effects of both acute and chronic stresses on the circulating levels of GH in the rainbow trout, the magnitude of the stress response being determined by the measurement of plasma ACTH and cortisol.…”

Section: Accepted August 2 1991mentioning

confidence: 99%

Effects of acute and chronic stress on the levels of circulating growth hormone in the rainbow trout, Oncorhynchus mykiss

Pickering

Pottinger

Sumpter

et al. 1991

General and Comparative Endocrinology

188

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“…Plasma GH levels have been reported to increase after injection stress in goldfish (Cook and Peter, 1984) and to remain unaffected in chum salmon after handling stress (Wagner and Mckeown, 1986). The role of GH in social interaction is considered by some authors as indirect, by increasing swimming activities or inducing defense of a larger territory, thereby increasing the encounter rate between opponents (Jonsson and Bjornsson, 1994).…”

Section: Discussionmentioning

confidence: 99%

Chronic social stress in rainbow trout: Does it promote physiological habituation?

Fernandes-de-Castilho

Pottinger

Volpato

2008

General and Comparative Endocrinology

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The effect of chronic social stress on growth, energetic substrates and hormones was tested in rainbow trout, Oncorhynchus mykiss. After a 14-day isolation period, the fish were paired for 8 days. In order to expose fish to chronic intermittent social contact during pairing, they were maintained in direct contact with each other during the first day. After that, a black plastic screen partition was introduced in each tank, preventing direct contact between animals. Every day the partition was removed for 30 minutes, allowing physical interaction between fish. At the end of pairing period, they were isolated again for 13 days. Fish were weighed and blood was sampled frequently during the experiment. Plasma levels of cortisol, growth hormone, glucose, total protein and free amino acids were quantified. Both dominants and subordinates had specific growth rate decreased during the pairing period and but only subordinates increased when the stressor was abolished (dominants: 0.32 ± 0.21 and 0.24 ± 0.41, subordinates: -0.77 ± 0.29 and 0.37 ± 0.31, respectively). Dominants showed a higher cortisol level one week after pairing condition had been abolished than subordinates (dominants: 56.76 ± 13.26, subordinates:31.89 ± 13.36). We conclude that chronic condition of intermittent social stress represents a stressful condition for animals of both hierarchical ranks and a treatment of one daily short direct contact between conspecifics does not promote habituation in fish, as mentioned for other stressors.social stress, energetic of stress, hierarchical dominance, rainbow trout, Oncorhynchus mykiss.

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Development of a salmon growth hormone radioimmunoassay

Cited by 70 publications

References 33 publications

The effect of starvation on growth and plasma growth hormone concentrations of rainbow trout, Oncorhynchus mykiss

The effect of starvation on growth and plasma growth hormone concentrations of rainbow trout, Oncorhynchus mykiss

Effects of acute and chronic stress on the levels of circulating growth hormone in the rainbow trout, Oncorhynchus mykiss

Chronic social stress in rainbow trout: Does it promote physiological habituation?

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