Development of a SCAR marker linked to the <i>I</i> gene in common bean

Melotto, Maeli; Afanador, L.; Kelly, James D.

doi:10.1139/g96-155

Cited by 152 publications

(132 citation statements)

References 16 publications

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“…The following molecular markers were used: the dominant SCAR marker SW13 690 , linked to the I gene (Melotto et al 1996); the dominant SCAR marker ROC11 420 , linked in repulsion phase to the recessive bc-3 resistance allele (Johnson et al 1997); the dominant CAP marker SCH20 1000 (obtained after Taq I digestion of amplification product), the dominant RAPD fragments OM2 1050 , OQ04 600, and the SCAR SQ4 1440 , linked to cluster Co-2 (Adam- Blondon et al 1994;Rodríguez-Suárez et al 2007Awale et al 2008); the dominant SCAR markers SI19 460 , SB12 350 , linked to Co-3/9 cluster (Miklas et al 2000;Méndez de Vigo et al 2005;Rodríguez-Suárez et al 2008). Specific molecular markers were used in breeding programs once they were reported.…”

Section: Molecular Marker Analysismentioning

confidence: 99%

Introgression and pyramiding into common bean market class fabada of genes conferring resistance to anthracnose and potyvirus

et al. 2011

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Anthracnose and bean common mosaic (BCM) are considered major diseases in common bean crop causing severe yield losses worldwide. This work describes the introgression and pyramiding of genes conferring genetic resistance to BCM and anthracnose local races into line A25, a bean genotype classified as market class fabada. Resistant plants were selected using resistance tests or combining resistance tests and marker-assisted selection. Lines A252, A321, A493, Sanilac BC6-Are, and BRB130 were used as resistance sources. Resistance genes to anthracnose (Co-2 C , Co-2 A252 and Co-3/9) and/or BCM (I and bc-3) were introgressed in line A25 through six parallel backcrossing programs, and six breeding lines showing a fabada seed phenotype were obtained after six backcross generations: line A1258 from

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Section: Molecular Marker Analysismentioning

confidence: 99%

Introgression and pyramiding into common bean market class fabada of genes conferring resistance to anthracnose and potyvirus

et al. 2011

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“…R3 is a resistance locus to bacterial brown spot (Crute and Pink, 1996). bc-1 2 and bc-3 are recessive genes for resistance to BCMV and I is a dominant resistance to the same virus (Nodari et al, 1993a;Johnson et al, 1997;Miklas et al, 2000a,b;Melotto et al, 1996). [AI-Arl-Lec] is the main locus coding for the amylase inhibitor-arcelin-lectin multigene family.…”

Section: Anthracnosementioning

confidence: 99%

“…Linked markers offer the only realistic opportunity to maintain and continue to utilize the I gene as a pyramided resistance gene in future bean cultivars. A marker tightly linked to the I gene, has been identified (Haley et al, 1994b;Melotto et al, 1996) and has been demonstrated in many labs to be effective across a wide range of germplasm from both gene pools. Breeders have used markers linked to the I gene to develop enhanced germplasm with the I þ bc-3 gene combination.…”

Section: Bcmv and Bean Common Mosaic Necrosis Virus (Bcmnv)mentioning

confidence: 99%

Tagging and mapping of genes and QTL and molecular marker-assisted selection for traits of economic importance in bean and cowpea

Kelly

Gepts

Miklas

et al. 2003

Field Crops Research

256

240

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Gepts, P.; Miklas, P. N.; and Coyne, D. P., "Tagging and mapping of genes and QTL and molecular marker-assisted selection for traits of economic importance in bean and cowpea" (2003 The bean map is approximately 1200 cM with some 500 markers and an additional 500 markers shared with other bean maps. The cowpea map spans 2670 cM with over 400 markers. In addition to molecular markers, both maps include map locations of defense genes and phenotypic traits for disease and insect resistance, seed size, color and storage proteins, pod color and those traits associated with the domestication syndrome in bean. Since the bean and cowpea maps were developed independently, LGs with the same number probably refer to non-syntenic groups. Map locations of major resistance genes in bean are revealing gene clusters on LGs B1, B4, B7, and B11 for resistance to bean rust, anthracnose, common bacterial blight and white mold. Gene tagging and marker-assisted selection for disease resistance has progressed to a point where the indirect selection for resistance to a number of major diseases is now routine in bean breeding programs both in the US and overseas. #

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“…Cultivars with I, combinations are immune to all strains of BCMV and BCMNV while i, bc-3 genotypes are resistant to all strains of BCMNV but susceptible to some strains of BCMV (Miklas et al, 1998). To develop complete resistance to both potyviruses, bean breeders have used indirect selection by deploying molecular markers linked to the I gene (Haley et al, 1994b;Melotto et al, 1996) to identify the dominant I gene in the presence of the epistatic bc-3 gene as the desirable I, bc-3 combination cannot be distinguished from i, bc-3 genotypes using direct inoculation with strains of BCMNV (Kelly, 1997).…”

Section: Introductionmentioning

confidence: 99%

Markers linked to the bc-3 gene conditioning resistance to bean common mosaic potyviruses in common bean

et al. 2005

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SummaryNecrotic strains of bean common mosaic potyviruses are becoming increasingly problematic in bean growing areas of Africa and Europe. Pyramiding epistatic resistance genes provides the most effective long-term strategy for disease control against all known strains of the virus. Indirect selection using tightly linked markers should facilitate the breeding of desired epistatic resistance gene combinations. In common bean, the most effective strategy for broad spectrum control of the bean common mosaic potyviruses is to combine I and bc-3 genes. We describe the use of near-isogenic lines and segregating populations from different gene pools combined with bulked segregant analysis to identify markers tightly linked with the recessive bc-3 gene that conditions resistance to all strains of bean common mosaic necrosis virus. We identified a RAPD marker, OG6 595 , linked at 3.7 cM from the bc-3, and the marker was used to confirm the location of bc-3 gene on bean linkage group B6. A codominant AFLP marker, E ACA M CGG -169/172 was identified and linked at 3.5 cM from the bc-3 and the AFLP and OG6 595 markers flanked the bc-3 gene. The AFLP marker was converted to the STS marker SE ACA M CGG -134/137 which showed co-segregation with the original AFLP marker. The 134 bp fragment associated with resistance was linked with the bc-3 gene present in a diverse group of bean genotypes except in two kidney bean lines. The OG6 595 marker mapped on B6 supported independence of bc-3 from the I gene located on B2, which provides the opportunity to readily combine both genes in a single bean cultivar for broad spectrum resistance to bean common mosaic potyviruses.

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Development of a SCAR marker linked to the I gene in common bean

Cited by 152 publications

References 16 publications

Introgression and pyramiding into common bean market class fabada of genes conferring resistance to anthracnose and potyvirus

Introgression and pyramiding into common bean market class fabada of genes conferring resistance to anthracnose and potyvirus

Tagging and mapping of genes and QTL and molecular marker-assisted selection for traits of economic importance in bean and cowpea

Markers linked to the bc-3 gene conditioning resistance to bean common mosaic potyviruses in common bean

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