Development of a screening assay to identify teratogenic and embryotoxic chemicals using the zebrafish embryo

Selderslaghs, Ingrid W.T.; Rompay, An R. Van; Coen, Wim De; Witters, Hilda

doi:10.1016/j.reprotox.2009.05.004

Cited by 258 publications

(155 citation statements)

References 60 publications

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“…Finally, the most active compounds 5e, 5m, and 5s were subjected to potential cardiac toxicity evaluation in a zebrafish (Danio rerio) model. 22 Compounds were analyzed with heart rate variations and were found to be safe to the embryos at 1 and 5 μM. By contrast, when tested at 20 μM, 2-(quinolin-4-yloxy)acetamides 5e, 5m, and 5s significantly reduced heart rate compared with control.…”

mentioning

confidence: 98%

2-(Quinolin-4-yloxy)acetamides Are Active against Drug-Susceptible and Drug-Resistant Mycobacterium tuberculosis Strains

Pissinate

Villela

Rodrigues-Junior

et al. 2016

ACS Med. Chem. Lett.

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2-(Quinolin-4-yloxy)acetamides have been described as potent in vitro inhibitors of Mycobacterium tuberculosis growth. Herein, additional chemical modifications of lead compounds were carried out, yielding highly potent antitubercular agents with minimum inhibitory concentration (MIC) values as low as 0.05 μM. Further, the synthesized compounds were active against drug-resistant strains and were devoid of apparent toxicity to Vero and HaCat cells (IC 50 s ≥ 20 μM). In addition, the 2-(quinolin-4-yloxy)acetamides showed intracellular activity against the bacilli in infected macrophages with action similar to rifampin, low risk of drug−drug interactions, and no sign of cardiac toxicity in zebrafish (Danio rerio) at 1 and 5 μM. Therefore, these data indicate that this class of compounds may furnish candidates for future development to, hopefully, provide drug alternatives for tuberculosis treatment.

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mentioning

confidence: 98%

2-(Quinolin-4-yloxy)acetamides Are Active against Drug-Susceptible and Drug-Resistant Mycobacterium tuberculosis Strains

Pissinate

Villela

Rodrigues-Junior

et al. 2016

ACS Med. Chem. Lett.

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“…Zebra fish embryos are complete organisms that allow their use as alternatives in acute fish tests for diverse effluent testing [12,[21][22][23].…”

Section: Discussionmentioning

confidence: 99%

“…Numerous authors have conducted tests concerning the early development stages of zebra fish, all revealing an increased sensitivity of this test to estimate: the maximum acceptable concentration of a toxin [2][3][4][6][7][8], developmental neurotoxicity [24], teratogenicity [5,23], embryotoxicity [3,25], and/or screening a large number of chemicals [10,13,14,26].…”

Section: Discussionmentioning

confidence: 99%

Use of Zebra Fish Eggs as Early Indicators of Aquatic Environmental Pollution

Moşneang¹,

Dumitrescu²,

Муселин³

et al. 2015

Pol. J. Environ. Stud.

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“…The zebrafish is widely used as a model species for vertebrate development studies and more recently has become embraced for use in the area of reproductive toxicology (Selderslaghs, Van Rompay, De Coen, & Witters, 2009;Alsop, Brown, & Van Der Kraak, 2007;Lister, Regan, Van Zwol, & Van Der Kraak, 2009). Large numbers of zebrafish can be housed in comparatively small spaces, which is beneficial as it is cost effective in terms of maintenance (Teraoka, Dong & Hiraga, 2003).…”

Section: Zebrafish (Danio Rerio)mentioning

confidence: 99%

Inhibition of spawning in zebrafish (Danio rerio): Adverse outcome pathways of quinacrine and ethinylestradiol

Cosme

Lister

Kraak

2015

General and Comparative Endocrinology

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INHIBITION OF SPAWNING IN ZEBRAFISH (Danio rerio): ADVERSE OUTCOME PATHWAYS OF QUINACRINE AND ETHINYLESTRADIOL Madelyne CosmeAdvisor: University of Guelph, 2014Professor. Glen Van Der KraakThis study examined the effects of estrogen receptor agonist, ethinylestradiol, and the phospholipase A 2 inhibitor, quinacrine, on the pathways controlling follicular recruitment, steroidogenesis, oocyte maturation and ovulation and spawning success in the adult zebrafish. Ethinylestradiol and quinacrine both inhibit spawning but did so through different adverse outcome pathways. Ethinylestradiol affected follicular recruitment (reduced ovarian size and reduction in the proportion of cortical alveolus, vitellogenic and mature follicle stages), steroidogenesis (reduced expression of aromatase) maturation (reduced luteinizing hormone receptor expression) and ovulation (reduced expression of cytosolic phospholipase A2 and the nuclear progesterone receptor). Quinacrine targeted ovulation via a reduction of the steroid 17α, 20β dihydroxy-4-prenen-3-one and expression of the prostaglandin metabolizing enzyme cyclooxygenase 2. Collectively, these studies suggest that a targeted assessment of reproductive endpoints can be used effectively to identify the adverse outcome pathways by which toxicants can impact reproductive fitness.iii ACKNOWLEDGEMENTS

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Development of a screening assay to identify teratogenic and embryotoxic chemicals using the zebrafish embryo

Cited by 258 publications

References 60 publications

2-(Quinolin-4-yloxy)acetamides Are Active against Drug-Susceptible and Drug-Resistant Mycobacterium tuberculosis Strains

2-(Quinolin-4-yloxy)acetamides Are Active against Drug-Susceptible and Drug-Resistant Mycobacterium tuberculosis Strains

Use of Zebra Fish Eggs as Early Indicators of Aquatic Environmental Pollution

Inhibition of spawning in zebrafish (Danio rerio): Adverse outcome pathways of quinacrine and ethinylestradiol

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