Development of a sensitive LC‐MS/MS method for simultaneous quantification of eleven constituents in rat serum and its application to a pharmacokinetic study of a Chinese medicine Shengmai injection

Zhan, Siyan; Shao, Qing; Fan, Xiaohui; Li, Zheng

doi:10.1002/bmc.3273

Cited by 23 publications

(18 citation statements)

References 34 publications

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“…Wang et al performed a simultaneous quantification of multiple components through both positive‐ and negative‐ion electrospray ionization using one IS. Indeed, their validation results indicate that the analysis could be performed reliably with one IS (Ezzeldin, Abo‐Talib, Tammam, & Shahat, ; Wang et al, ; Zhan, Shao, Fan, & Li, ), which is in accordance with our findings.…”

Section: Resultssupporting

confidence: 92%

Development and validation of an LC–MS/MS method for the simultaneous quantification of seven constituents in rat plasma and application in a pharmacokinetic study of the Zaoren Anshen prescription

Zhao

Zhang

et al. 2017

Biomedical Chromatography

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A sensitive, specific and accurate liquid chromatography-tandem mass spectrometry (LC-MS/MS) method was developed and validated for the simultaneous determination of seven constituents of the Zaoren Anshen prescription (ZAP) in rat plasma after oral administration of the ZAP: spinosin, salvianic acid A, 6'''-feruloylspinosin, protocatechualdehyde, salvianolic acid B, schisandrin and deoxyschisandrin. The plasma samples and the internal standard (IS) sulfamethoxazole were extracted using acetonitrile. Chromatographic separation was performed with an Agilent HC-C column using a gradient elution profile and a mobile phase consisting of 0.01% formic acid in water (A) and acetonitrile (B). The analytes were quantified simultaneously in a single run using an ion trap mass spectrometer operated in the multiple reaction monitoring mode and electrospray ion-source polarity in the positive and negative modes. The calibration curves for spinosin, salvianic acid A, 6'''-feruloylspinosin, protocatechualdehyde, salvianolic acid B, schisandrin and deoxyschisandrin were linear over the concentration ranges of 2.90-1160, 2.50-1000, 1.80-720, 0.65-260, 2.50-1000, 8.00-1600 and 1.30-520 ng/mL, respectively. The intra- and inter-day precisions in terms of relative standard deviation were <18.9%, and the accuracies in terms of relative error were within ±14.2%. Consequently, the proposed method was successfully applied to the pharmacokinetic analysis of these seven major active compounds in rats administered ZAP. These results will facilitate research aiming to predict the effectiveness of the optimal dose of ZAP and might be beneficial for the therapeutic use of ZAP in the clinical setting.

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Section: Resultssupporting

confidence: 92%

Development and validation of an LC–MS/MS method for the simultaneous quantification of seven constituents in rat plasma and application in a pharmacokinetic study of the Zaoren Anshen prescription

Zhao

Zhang

et al. 2017

Biomedical Chromatography

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“…Previous reports showed that time to reach peak concentration ( T max ) is 1 h for BHCl (Liu et al ., ), 0.5 h for emodin and FA (Gong et al ., ; Wen et al ., ), 0.25 h for THSG (Zhao et al ., ), 0.6 h for ginsenoside Rg1 and 1.5 h for ginsenoside Rb1 (Xu et al ., ). The pharmacokinetics of Re and Rg1 are similar because they both belong to the 20 ( S )‐protopanaxatriol type according to their structures (Li et al ., ; Zhan et al ., ). Hence, blood samples of 8 mL were taken from the abdominal aorta 2 h after oral administration and kept standing for 30 min to obtain plasma.…”

Section: Methodsmentioning

confidence: 97%

Metabolite identification of seven active components of Huan‐Nao‐Yi‐Cong‐Fang in rat plasma using high‐performance liquid chromatography combined with hybrid ion trap/time‐of‐flight mass spectrometry

Wang

Liu

et al. 2015

Biomedical Chromatography

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Huan-Nao-Yi-Cong-Fang (HNYCF) is a potential prescription in treating Alzheimer's disease. Seven constituents [ferulic acid (FA), 2,3,5,4'-tetrahydroxystilbene-2-O-β-d-glucoside (THSG), berberine hydrochloride (BHCl), emodin, ginsenoside Rg1 (Rg1), ginsenoside Re (Re) and ginsenoside Rb1 (Rb1)] have been used as quality chemical markers of HNYCF owing to their biological significance and high contents in crude plant materials. This study explored the metabolites of the seven bioactive components in rat plasma to give useful data for further study of the action mechanism of HNYCF. LC/MS-IT-TOF was used to simultaneously characterize the metabolites of the seven components. Using the combination of MetID Solution 1.0 software and accurate mass measurements, the metabolites of HNYCF were reliably characterized. Their structures were elucidated based on the accurate MS(2) spectra and comparisons of their changes in accurate molecular masses and fragment ions with those of parent compounds. A total of five parent active compounds (BHCl, emodin, Rg1, Rb1 and Re) and 10 metabolites were found from the rat plasma 2 h after oral administration of HNYCF dosage, of which two metabolites of emodin were observed for the first time. The proposed metabolic pathways of the bioactive components in the rat plasma are helpful for further studies on the pharmacokinetics and real active compound forms of this drug.

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“…Also, an LLOQ of 1 ng/mL for the three lignans that was reported was at least 20‐times higher than the presently determined limit. The other methods were less sensitive, with LLOQs ranging from 0.23–1.2 ng/mL for schizandrin and 0.26–1.48 ng/mL for gomisin N . In comparison, our approach was more sensitive for three lignans in plasma (LLOQ of 0.05 ng/mL for all three lignans using the LLE method with the small amount of 0.1 mL plasma).…”

Section: Resultsmentioning

confidence: 81%

“…Analytical techniques that have been used to analyze the three lignans include LC–MS , LC–MS/MS , UHPLC–MS/MS , and GC–MS . Among these studies, only two publications suggested an analytical method capable of simultaneously analyzing the three lignans .…”

Section: Introductionmentioning

confidence: 99%

A sensitive UHPLC–MS/MS method for the simultaneous quantification of three lignans in human plasma and its application to a pharmacokinetic study

Kim

Shin

Seong-Moon

et al. 2017

J of Separation Science

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The aim of this study was to develop an analytical method to simultaneously analyze schizandrin, schizandrol B, and gomisin N lignans in human plasma using ultra high performance liquid chromatography with tandem mass spectrometry. The three lignans were separated using a mobile phase of water and acetonitrile containing 0.02% acetic acid equipped with a Kinetex C column (2.1 mm × 50 mm, 1.7 μm). This analysis was achieved by multiple reaction monitoring mode in an electrospray interface. The mass transitions were m/z 433.1→384.0 for schizandrin, 398.8→367.8 for schizandrol B, and 400.6→299.8 for gomisin N. Liquid-liquid extraction with methyl tert-butyl ether was used to obtain the three lignans. The chromatograms showed high resolution, sensitivity, and selectivity with no interference with plasma constituents. The calibration curves for the three lignans in human plasma were 0.05-50 ng/mL and displayed excellent linearity with correlation coefficients greater than 0.99. Precision for all three lignans was within 11.23%. The accuracy was 88.3-99.0% for schizandrin, 90.6-103.4% for schizandrol B, and 90.2-103.5% for gomisin N. The developed simultaneous analytical method satisfied the criteria of international guidance and could be successfully applied to the pharmacokinetic study of three lignans after oral administration of Schisandrae Fructus extract powder to humans.

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Development of a sensitive LC‐MS/MS method for simultaneous quantification of eleven constituents in rat serum and its application to a pharmacokinetic study of a Chinese medicine Shengmai injection

Cited by 23 publications

References 34 publications

Development and validation of an LC–MS/MS method for the simultaneous quantification of seven constituents in rat plasma and application in a pharmacokinetic study of the Zaoren Anshen prescription

Development and validation of an LC–MS/MS method for the simultaneous quantification of seven constituents in rat plasma and application in a pharmacokinetic study of the Zaoren Anshen prescription

Metabolite identification of seven active components of Huan‐Nao‐Yi‐Cong‐Fang in rat plasma using high‐performance liquid chromatography combined with hybrid ion trap/time‐of‐flight mass spectrometry

A sensitive UHPLC–MS/MS method for the simultaneous quantification of three lignans in human plasma and its application to a pharmacokinetic study

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