Development of a sensitive phage-mimotope and horseradish peroxidase based electrochemical immunosensor for detection of O,O-dimethyl organophosphorus pesticides

Shi, Ruirui; Zou, Wenting; Zhao, Zhiling; Wang, Guanqun; Guo, Min; Ai, Shiyun; Zhou, Qing; Zhao, Fang; Yang, Zhengyou

doi:10.1016/j.bios.2022.114748

Cited by 22 publications

(6 citation statements)

References 48 publications

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“…Albeit the traditional analytical methods for fenitrothion detection including HPLC-photodiode array, ultra-high performance liquid chromatography, and GC-flame photometric detector possessed good sensitivity and high accuracy, ,, the detection process generally suffered from specific drawbacks requiring expensive instruments, large quantities of chemicals, and skilled personnel . Meanwhile, some researchers employed antigen/antibody-modified fluorescent/electrochemical immunosensors for the ultrasensitive determination of fenitrothion. , Although the introduction of antigens/antibodies as recognition molecules offered better sensitivity with lower LOD, they may have sophisticated manufacturing processes, high screening costs, and poor stability in adverse conditions. In addition, researchers have developed various fluorescent sensors based on Ni,N-CDs/Fe 3 O 4 @ZIF-8, Eu 3+ -functionalized UiO-67-bpydc or Zn 4 O(BDC) 3 ·DEF MOFs materials for detection of fenitrothion yet with a narrow detection range and poor LOD. ,, These MOFs are poorly dispersed in solution due to their inherent inorganic coordination material nature, making the development of MOFs-based sensors challenging in the stability and reliability of various applications .…”

Section: Resultsmentioning

confidence: 99%

“…9 Meanwhile, some researchers employed antigen/antibody-modified fluorescent/electrochemical immunosensors for the ultrasensitive determination of fenitrothion. 39,40 Although the introduction of antigens/antibodies as recognition molecules offered better sensitivity with lower LOD, they may have sophisticated manufacturing processes, high screening costs, and poor stability in adverse conditions. In addition, researchers have developed various fluorescent sensors based on Ni,N-CDs/Fe 3 O 4 @ZIF-8, Eu 3+ -functionalized UiO-67bpydc or Zn 4 O(BDC) 3 •DEF MOFs materials for detection of fenitrothion yet with a narrow detection range and poor LOD.…”

Section: Optimization Of Critical Parametersmentioning

confidence: 99%

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Ultrasensitive Fluorescence Platform Based on AgNPs In Situ-Incorporated Zr-MOFs for the Detection of Organophosphorus Pesticides

Wang,

Pan,

Wang

et al. 2023

ACS Appl. Mater. Interfaces

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Organophosphorus pesticides (OPPs) are extensively used in agricultural production, and the contamination caused by their residues has raised significant concerns regarding potential threats to human health. Herein, a novel fluorescence nanoprobe based on an enzyme-mediated silver nanoparticle-modified metal organic framework (AgNPs@PCN-224) was successfully prepared for the rapid detection of OPPs. Initially, AgNPs@PCN-224 were synthesized by reducing silver nitrate (AgNO3) using sodium borohydride (NaBH4) embedded into luminescent PCN-224. This triggered the inner filter effect, leading to fluorescence quenching. Meanwhile, under the catalysis of acetylcholinesterase (AChE) and choline oxidase (CHO), acetylcholine (ATCh) was decomposed to hydrogen peroxide (H2O2), which could destroy AgNPs to form Ag+ released from PCN-224 for fluorescence recovery. Instead, fenitrothion, an OPP, inhibited AChE activity, allowing the quenched fluorescence to be reactivated. Under the current optimum conditions, the fluorescence intensity had a good correlation (Y = −728.5370X + 2178.4248, R 2 = 0.9869) over a dynamic range of fenitrothion concentrations from 0.1 to 500 ng/mL, with an LOD of 0.037 ng/mL. In addition, the anti-interference ability and robustness of the proposed sensor was verified for the monitoring of fenitrothion in tea with recoveries of 87.67–103.72% and the relative standard deviations (RSD) < 5.43%, indicating that the system has excellent prospects for OPP determination in practical applications. Furthermore, this work provides a universal platform for screening other enzyme inhibitors to detect OPPs.

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Section: Resultsmentioning

confidence: 99%

Section: Optimization Of Critical Parametersmentioning

confidence: 99%

Ultrasensitive Fluorescence Platform Based on AgNPs In Situ-Incorporated Zr-MOFs for the Detection of Organophosphorus Pesticides

Wang,

Pan,

Wang

et al. 2023

ACS Appl. Mater. Interfaces

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“…NB-CQDs were synthesized according to previous work with slight modifications. , 1.00 g of citric acid, 1.00 g of p -phenylenediamine, and 1.00 g of borax were added to 50.0 mL of deionized water, stirred until completely dissolved, and then transferred to a 100 mL high pressure reactor (Teflon lined) and reacted at 180 °C for 5 h. After the reaction, the reactor was allowed to cool naturally to room temperature. The resulting crude product dispersion was then filtered through a 0.220 μm membrane and dialyzed in water (500 Da) for 24 h. The dispersion obtained after purification was the dispersion of the NB-CQDs.…”

Section: Methodsmentioning

confidence: 99%

Quantification of Glycated Hemoglobin in Total Hemoglobin by a Simultaneous Dual-Signal Acquisition Approach

Wang,

Xiong,

Zhang

et al. 2024

ACS Sens.

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The glycated hemoglobin (HbA 1c ) level, which is defined as the ratio of HbA 1c to total hemoglobin (tHb, including glycated and unglycated hemoglobin), is considered one of the preferred indicators for diabetes monitoring. Generally, assessment of the HbA 1c level requires separate determination of tHb and HbA 1c concentrations after a complex separation step. This undoubtedly increases the cost of the assay, and the loss or degradation of HbA 1c during the separation process results in a decrease in the accuracy of the assay. Therefore, this study explored a dual-signal acquisition method for the one-step simultaneous evaluation of tHb and HbA 1c . Quantification of tHb: graphene adsorbed carbon quantum dots and methylene blue were utilized as the substrate material and linked to the antibody. tHb was captured on the substrate by the antibody. The unique heme group on tHb catalyzed the production of •OH from H 2 O 2 to degrade methylene blue on the substrate, and a quantitative relationship between the tHb concentration and the methylene blue oxidation current signal was constructed. Quantification of HbA 1c : complex labels with HbA 1c recognition were made of ZIF-8−ferrocene−gold nanoparticles−mercaptophenylboronic acid. The specific recognition of the boronic acid bond with the unique cis-diol structure of HbA 1c establishes a quantitative relationship between the oxidation current of the label-loaded ferrocene and the concentration of HbA 1c . Thus, the HbA 1c level can be assessed with only one signal readout. The sensor exhibited extensive detection ranges (0.200−600 ng/mL for tHb and 0.100−300 ng/mL for HbA 1c ) and low detection limits (4.00 × 10 −3 ng/mL for tHb and 1.03 × 10 −2 ng/mL for HbA 1c ).

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“…The phage that mimic OP was decorated with anti-M13 mAb-HRP for signal output. [94] By taking advantage of the unique structure of M13, plenty of anti-M13 mAb-HRP can be decorated, resulting in amplified signal and a LOD of down to 0.003-0.014 ng/mL [98]. Although M13 phage-based electrochemical sensor provided simple and label-free detection for sensitive analysis, two issues should be taken into consideration.…”

Section: Electrochemical Sensingmentioning

confidence: 99%

M13 phage: a versatile building block for a highly specific analysis platform

Wang

Cao

et al. 2023

Anal Bioanal Chem

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Viruses are changing the biosensing and biomedicine landscape due to their multivalency, orthogonal reactivities, and responsiveness to genetic modifications. As the most extensively studied phage model for constructing a phage display library, M13 phage has received much research attention as building blocks or viral scaffolds for various applications including isolation/separation, sensing/probing, and in vivo imaging. Through genetic engineering and chemical modification, M13 phages can be functionalized into a multifunctional analysis platform with various functional regions conducting their functionality without mutual disturbance. Its unique filamentous morphology and flexibility also promoted the analytical performance in terms of target affinity and signal amplification. In this review, we mainly focused on the application of M13 phage in the analytical field and the benefit it brings. We also introduced several genetic engineering and chemical modification approaches for endowing M13 with various functionalities, and summarized some representative applications using M13 phages to construct isolation sorbents, biosensors, cell imaging probes, and immunoassays. Finally, current issues and challenges remaining in this field were discussed and future perspectives were also proposed.

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Development of a sensitive phage-mimotope and horseradish peroxidase based electrochemical immunosensor for detection of O,O-dimethyl organophosphorus pesticides

Cited by 22 publications

References 48 publications

Ultrasensitive Fluorescence Platform Based on AgNPs In Situ-Incorporated Zr-MOFs for the Detection of Organophosphorus Pesticides

Ultrasensitive Fluorescence Platform Based on AgNPs In Situ-Incorporated Zr-MOFs for the Detection of Organophosphorus Pesticides

Quantification of Glycated Hemoglobin in Total Hemoglobin by a Simultaneous Dual-Signal Acquisition Approach

M13 phage: a versatile building block for a highly specific analysis platform

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