Development of a set of three real-time loop-mediated isothermal amplification (LAMP) assays for detection of Bacillus anthracis, the causative agent of anthrax

Banger, Swati; Pal, Vijai; Tripathi, Nagesh K.; Goel, Ajay

doi:10.1007/s12223-021-00869-x

Cited by 2 publications

(1 citation statement)

References 26 publications

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“…For example, Wang et al [ 54 ] reported the detection of Fusarium proliferatum with a LOD of 10 pg/µL in 30 min. Additionally, a SYBR Green I-based LAMP assay was used for the detection of Listeria monocytogenes ( L. monocytogenes ), [ 55 ] Enterocytozoon hepatopenaei , [ 56 ] Milk vetch dwarf virus [ 57 ], and Bacillus anthracis [ 58 ]. Recently, Oliveira et al [ 59 ] reported RT-LAMP-based detection of SARS-CoV-2 in a fully integrated and automated POC device.…”

Section: Lamp Detection Methodsmentioning

confidence: 99%

LAMP-Based Point-of-Care Biosensors for Rapid Pathogen Detection

2022

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Seeking optimized infectious pathogen detection tools is of primary importance to lessen the spread of infections, allowing prompt medical attention for the infected. Among nucleic-acid-based sensing techniques, loop-mediated isothermal amplification is a promising method, as it provides rapid, sensitive, and specific detection of microbial and viral pathogens and has enormous potential to transform current point-of-care molecular diagnostics. In this review, the advances in LAMP-based point-of-care diagnostics assays developed during the past few years for rapid and sensitive detection of infectious pathogens are outlined. The numerous detection methods of LAMP-based biosensors are discussed in an end-point and real-time manner with ideal examples. We also summarize the trends in LAMP-on-a-chip modalities, such as classical microfluidic, paper-based, and digital LAMP, with their merits and limitations. Finally, we provide our opinion on the future improvement of on-chip LAMP methods. This review serves as an overview of recent breakthroughs in the LAMP approach and their potential for use in the diagnosis of existing and emerging diseases.

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