Development of a simple and reliable high-performance liquid chromatography–tandem mass spectrometry approach to simultaneously detect grains specified in food allergen labeling regulation on processed food commodities

Seki, Yusuke; Nakamura, Kôsuke; Arimoto, Chisato; Kikuchi, Hiroyuki; Yamakawa, Hirohito; Nagai, Hirokazu; Ito, Tetsuro; Akiyama, Hiroshi

doi:10.1016/j.chroma.2021.461877

Cited by 17 publications

(11 citation statements)

References 30 publications

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“…Detection of the synthetic targeted peptides was based on our previously developed LC-MS/MS methods ( Seki et al, 2021 ). All targeted peptides were initially analysed at a very low concentration of 10 ng/mL, and the concentration was increased by 10 times for further analyses.…”

Section: Resultsmentioning

confidence: 99%

“…Sample preparation for LC-MS/MS analysis as described in Seki et al (2021) was used with some modifications. Samples were ground using a food processor, and the ground sample (0.5 g) was solubilized in 5 mL of 100 mM Tris-HCl buffer (pH 8.2) containing 4 M urea and 0.1 M dithiothreitol.…”

Section: Methodsmentioning

confidence: 99%

“…HPLC and MS/MS conditions were described in our previous study ( Seki et al, 2021 ). Liquid chromatography was run with a mobile phase of 0.1% (v/v) formic acid (solution A) and 0.1% (v/v) formic acid/acetonitrile (solution B) at flow rate of 0.3 mL/min under gradient conditions: 1% (v/v) solution B at 0 min, 1% (v/v) solution B at 4 min, 60% (v/v) solution B at 16.5 min, 95% (v/v) solution B at 20 min, 95% (v/v) solution B at 25 min, 1% (v/v) solution B at 25.1 min, and 1% (v/v) solution B at 35 min.…”

Section: Methodsmentioning

confidence: 99%

“…In this study, we developed a reliable and highly sensitive analytical method to simultaneously detect walnuts and almonds contained in processed foods using LC-MS/MS by applying the simultaneous analytical method for wheat and buckwheat that we previously reported ( Seki et al, 2021 ). We confirmed the specificity using raw materials containing major allergens, including nuts, and verified the differences between multiple walnut and almond varieties.…”

Section: Introductionmentioning

confidence: 99%

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Development of a simple and reliable LC-MS/MS method to simultaneously detect walnut and almond as specified in food allergen labelling regulations in processed foods

Torii

Seki

Arimoto

et al. 2023

Current Research in Food Science

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Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Development of a simple and reliable LC-MS/MS method to simultaneously detect walnut and almond as specified in food allergen labelling regulations in processed foods

Torii

Seki

Arimoto

et al. 2023

Current Research in Food Science

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“…In Japan, Nagai et al developed a method for analyzing buckwheat allergens using LC-MS/MS 25) . Seki et al developed an LC-MS/MS analysis method targeting peptides derived from trypsin digestion, i.e., glutenin of wheat including wheat, rye, barley, and oats and 13S globulin of buckwheat 26) . The LC-MS/MS method enables specific and simultaneous detection of a plurality of allergens, and is an effective confirmation test, as with real-time PCR.…”

Section: Lc-ms/ms Methodsmentioning

confidence: 99%

Japanese Food Allergy-Labeling System and Comparison with the International Experience; Detection and Thresholds

Akiyama

Adachi

2021

Food Safety

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In the Japanese allergy-labeling system, food labeling is mandated for 7 specific ingredients (egg, cow's milk, wheat, buckwheat, peanut, shrimp, and crab) and recommended for 21 food ingredients in reference to case numbers of actual illness and the degree of seriousness. To monitor the validity of the labeling system, official methods for the detection of specific ingredient proteins in processed foods were developed. The official methods consist of ELISA methods for screening, and western blot methods for egg and milk, and PCR methods for wheat, buckwheat, peanut, shrimp/prawn, and crab as confirmation tests. The official methods consist of ELISA methods for screening, and western blot methods for egg and milk, and PCR methods for wheat, buckwheat, peanut, shrimp/prawn, and crab as confirmation tests. Threshold amounts (a few mg/ kg) for labeling were set based on the approach of the analytical detections. Any foods containing protein allergens should be labeled if these contain allergens at greater than 10 ppm (mg/kg). Validation protocol criteria were established to standardize the Japanese official method. Food Safety Commission of Japan conducted a risk assessment of egg as a specific ingredient and judged that current labeling system for foods containing allergens is generally appropriate for "eggs". In the future, it is important to accumulate necessary scientific knowledge in order to carry out food health impact assessment including further refinement. The Japanese experience and knowledge of food allergy-labeling system would contribute to harmonize international labeling guidelines to protect allergic consumers globally.

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Recent Development on the Determination of Allergens in Food

Nitride,

Ferranti,

D'Auria

et al. 2024

Encyclopedia of Analytical Chemistry

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Risk assessment of unintended food allergens, contaminants in the food production chain, relies on the availability of analytical methods capable of providing accurate measurements. DNA‐ and immune‐based assays are first action methods providing within a short time frame information about the measurand, expressed as milligrams of total protein of the allergenic ingredient per kilogram of food product. On the other hand, isotope dilution mass spectrometry ( IDMS ) serves as a confirmatory method by providing peptide absolute quantification with a small measurement uncertainty, achieved by removing instrumental fluctuations. This analytical approach is a step forward to metrological traceable methods for food allergen analysis. All the detection methods can measure DNA, single peptides, or proteins, while the allergenic consumer protection and the action levels to support the quantitative risk assessments are based on the total mass of the allergenic food proteins. This article provides an overview of the analytical methods to be used in the frame of food allergen detection with a focus on methodological advancement.

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Development of a simple and reliable high-performance liquid chromatography–tandem mass spectrometry approach to simultaneously detect grains specified in food allergen labeling regulation on processed food commodities

Cited by 17 publications

References 30 publications

Development of a simple and reliable LC-MS/MS method to simultaneously detect walnut and almond as specified in food allergen labelling regulations in processed foods

Development of a simple and reliable LC-MS/MS method to simultaneously detect walnut and almond as specified in food allergen labelling regulations in processed foods

Japanese Food Allergy-Labeling System and Comparison with the International Experience; Detection and Thresholds

Recent Development on the Determination of Allergens in Food

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