Development of a simple and low-cost automated cell sheet harvesting system for tissue engineering

Leangarun, Teema; Chumroenrattanakorn, Phongsathorn; Koolpirak, Diew; Viravaidya-Pasuwat, Kwanchanok

doi:10.1109/aim.2017.8014141

Cited by 1 publication

(2 citation statements)

References 9 publications

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“…Scaffold-free cell sheets are self-supporting layers that consist of pure cells and their native extracellular matrix (ECM) networks, serving as ideal building blocks for the reconstruction of damaged tissues. − Compared with cell suspension, cell sheets overcome the challenges in cellular survival and accumulation at the target sites and have therapeutic effects. , Without using the exogenous bioscaffold, it greatly reduces the risks in the emergence of foreign body responses and released metabolic stress. − Contributing to the advance of a commercial method, the classical cell sheet can be harvested from a flat thermally responsive culture dish with a poly( N -isopropylacrylamide) (polyNIPAAm) coating, promoting the development of cell sheet engineering in regenerative medicine, such as repairs of the cornea, gums, blood vessels, and cartilage …”

Section: Introductionmentioning

confidence: 99%

“…Given the biomimetic morphology and native ECM likely biochemistry, hereon, the cell-imprinting technique was integrated into cell sheet engineering. ,,, Different from the traditional cell-imprinting technique, this work uses the whole organ as the template, the natural tissue-imprinting method, for the first time. As shown in Figure , the cellular shape, natural cell organization, and alignment can be well recapitulated on a collagen layer through the cell-imprinting strategy.…”

Section: Introductionmentioning

confidence: 99%

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Natural Tissue-Imprinted Biointerface for the Topographical Education of a Biomimetic Cell Sheet

Yao¹,

Meng³

et al. 2022

Langmuir

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Cell sheet engineering as a cell-based scaffold-free therapy is promising in tissue engineering, allowing precise transforming treatments for various tissue damage. However, the current cutting-edge techniques are still hampered by the difficulty in mimicking the natural tissue organizations and the corresponding physiological functions. In this work, cell-imprinting technology using the natural tissue as a template was proposed to rationally educate the cellular alignment in the cell sheet. Through this technique, we obtained temporary templates with morphological structure complementary to native tissues and then directly transferred the structure on the template to the collagen layer on a photothermally convertible substrate by secondary imprinting replication. The resultant biomimetic interface was used for cell culture and release to obtain a cell sheet with a texture similar to the natural tissue morphology. Different from conventional photolithography, the natural tissue-imprinted biointerface guides the geometry of cell sheets in the way of natural principles instead of stereotyped or overuniform cell organization. Simultaneously, a near-infrared laser (NIR) was used to irradiate the photothermally responsive substrate to obtain complete cell sheets efficiently and nondestructively. The natural tissue-educated myocardium cell sheets exhibited good physiological activity and biomimetic biofunctions, such as mechanical properties and physiological performances. This approach might open an inspiring prospect in regenerative medicine and offer a new approach to realizing the biomimetic tissue construction.

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