Development of a single-use microbioreactor for cultivation of microorganisms

Topology optimized microbioreactors

“…Adding this variable into the simulation is possible as it basically adds another diffusion term. In practice bubbleless aeration via the top/bottom of the reactor could be a suitable solution for oxygen supply, a technique that has been used widely in microbioreactor designs (Lee et al, 2006;Schäpper et al, 2010;Zhang et al, 2005). If one assumes, as in this model, that the reactor height is only a few tens of micrometers, and that, for example, both the floor and the ceiling of the reactor consisted of a membrane for bubbleless aeration, then it might well be possible to supply sufficient oxygen to the cells.…”

“…There has recently been considerable interest in the development of microbioreactors (volume below 1 mL) that allow experimentation with microorganisms at microscale under well-controlled conditions (Lee et al, 2006;Schäpper et al, 2009Schäpper et al, , 2010Szita et al, 2005;Zhang et al, 2007). When performing fermentation experiments at microscale, the increased design flexibility offered by the microfluidic systems enables a wide range of reactor configurations, potentially leading to higher productivities compared to traditional reactor designs.…”

Microfluidic Bioreactors for Cell Culturing: A Review

“…Most of the active mixers reported in the literature are used in microbial based microbioreactors [34,[103][104][105]. Recently, a microbioreactor for the fermentation of yeast cells and their mixing was achieved by using a free floating micro magnetic stirrer bar (3 mm length, 1.2 mm diameter) [106]. The stirrer was actuated from above to create random chaotic motion inside the reactor chamber which facilitated effective mixing.…”

A fully online sensor‐equipped, disposable multiphase microbioreactor as a screening platform for biotechnological applications

“…MBRs were first introduced by the Jensen group (Szita et al, 2005;Zanzotto et al, 2004;Zhang et al, 2006) and have been developed as a screening tool for bacterial and mammalian/human cell cultivation systems, especially for biotechnological, pharmaceutical and medical process development and optimization (Krull & Peterat, 2016;Schäpper, Stocks, Szita, Lantz, & Gernaey, 2010). The main research foci include the feasibility of online analytic integration and strain-dependent biological reaction kinetic parameter measurement.…”