Development of a special alligator forceps for removal of pulmonary heartworms in very small dogs.

Ishihara, Katsuya; Sasaki, Yoshihide; Kitagawa, Hitoshi; Kitao, Shinji; Takehashi, Nobuo

doi:10.1292/jvms1939.52.159

Cited by 1 publication

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“…Two antigenic extracts from D. immitis adult worms were obtained: one from a naturally infected dog (DiSA) and one from a dog naturally infected and treated with doxycycline (10 mg/kg BID for 15 days) (tDiSA). In both cases, worms were removed directly from the right ventricle using the technique of Ishiara et al [18] with some modifications. Worms were washed, macerated and sonicated (three cycles of 70 kHz, 30 s) in sterile saline solution.…”

Section: Methodsmentioning

confidence: 99%

Angiogenic response in an in vitro model of dog microvascular endothelial cells stimulated with antigenic extracts from Dirofilaria immitis adult worms

Zueva

Morchón

Carretón³

et al. 2019

Parasites Vectors

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Background Angiogenesis can occur under pathological conditions when stimuli such as inflammation, vascular obstruction or hypoxia exist. These stimuli are present in cardiopulmonary dirofilariosis ( Dirofilaria immitis ). The aim of this study was to analyze the capacity of D. immitis antigens to modify the expression of angiogenic factors and trigger the formation of pseudocapillaries (tube-like structures) in an in vitro model of endothelial cells. Methods The expression of VEGF-A, sFlt, mEndoglin and sEndoglin in cultures of canine microvascular endothelial cells stimulated with extract of adult worms of D. immitis obtained from an untreated dog (DiSA) and from a dog treated for 15 days with doxycycline (tDiSA), was determined by using commercial kits. The capacity of pseudocapillary formation was evaluated analyzing cell connections and cell groups in Matrigel cell cultures stimulated with DiSA and tDiSA. In both cases non-stimulated cultures were used as controls. Results First, we demonstrated that worms obtained from the dog treated with doxycycline showed a significantly lower amount of Wolbachia (less than 60%) than worms removed from the untreated dog. Only DiSA was able to significantly increase the expression of the proangiogenic factor VEGF-A in the endotelial cells cultures. None of the D. immitis extracts modified the expression of sFlt. tDiSA extract was able to modify the expression of the endoglins, significantly decreasing the expression of the pro-angiogenic mEndoglin and increasing the anti-angiogenic sEndoglin. The formation of pseudocapillaries was negatively influenced by tDiSA, which reduced the organization and number of cellular connections. Conclusions The ability of antigens from adult D. immitis worms to modify the expression of pro and anti-angiogenic factors in endotelial cell cultures was demonstrated, as well as the trend to form pseudocapillaries in vitro . The capacity of stimulation may be linked to the amount of Wolbachia present in the antigenic extracts.

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Section: Methodsmentioning

confidence: 99%