Development of a sugar isomerase cascade to convert D-xylose to rare sugars

Tang, Hengtao; Zhang, Zheng; Chen, Zhi; Ju, Xin; Li, Liangzhi

doi:10.1016/j.mcat.2022.112672

Cited by 5 publications

(6 citation statements)

References 36 publications

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“…Construction of an enzymatic cascade reaction to convert xylose Our previous study obtained an OsRpiB mutant that could efficiently transform xylose to D-xylulose, 27 and the initiation of enzymatic cascade development was reducing D-xylulose to D-arabitol to further improve the conversion rate of xylose. Four kinds of microbial ArDH were found with corresponding activity by literature research and are summarized in Table 1.…”

Section: Resultsmentioning

confidence: 99%

“…Under the optimal reaction conditions, pure D-xylose of 70 mmol L −1 was converted to 21 mmol L −1 D-xylulose and 30 mmol L −1 D-arabitol, with a total conversion rate of 73% after 36 h. For comparison, the conversion rates of single isomerization and isomerization-isomerization cascade were 22% and 43% respectively. 27 The enzymatic hydrolysis of lignocellulose generated 48 mmol L −1 xylose, and 10 mmol L −1 D-xylulose and 21 mmol L −1 D-arabitol were prepared finally, with a total conversion rate of 65%. The lower conversion rate of the five-enzyme cascade from lignocellulose and shorter reaction time might be due to the inhibition effect of chemicals from lignocellulose hydrolysate.…”

Section: Optimization Of the Isomerization-redox Cascadementioning

confidence: 99%

“…(OsRpiB) was identified with unique substrate scope and can selectively convert xylose from mixtures of glucose and other biomass monosaccharides. 25,26 Here, enzymatic cascades were designed on the basis of enhanced OsRpiB activity towards xylose by directed evolution 27 and employed sugar reductases to convert xylulose further to sugar alcohols. A coenzyme regeneration system using glucose was further added to recycle reduced nicotinamide adenine dinucleotide and convert glucose simultaneously.…”

Section: Introductionmentioning

confidence: 99%

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Development of an enzymatic cascade to systematically utilize lignocellulosic monosaccharide

Tang

Chen

Yu³

et al. 2022

J Sci Food Agric

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BACKGROUND The fermentation valorization of two main lignocellulosic monosaccharides, glucose and xylose, is extensively developed; however, it is restricted by limited yield and process complexity. An in vitro enzymatic cascade reaction can be an alternative approach. RESULTS In this study, a three‐stage, five‐enzyme cascade was developed to convert pretreated biomass to valuable chemicals. First, a ribose‐5‐phosphate isomerase B mutant isomerized xylose to d‐xylulose with high substrate specificity, and a d‐arabinose dehydrogenase continued to reduce d‐xylulose to d‐arabitol. Simultaneously, glucose was utilized for the coenzyme regeneration catalyzed by a glucose dehydrogenase, generating useful gluconic acid and achieving 73% of total conversion rate after 36 h. Then, six kinds of pretreated biomass lignocellulose were hydrolyzed by cellulase and hemicellulase, and corn cob was identified as the initial substrate for providing the highest monosaccharide content. A 65% conversion rate of the lignocellulosic xylose was obtained after 24 h. CONCLUSIONS This study presents a proof of concept to convert main lignocellulosic monosaccharides systematically by an enzymatic cascade at stoichiometric ratio. © 2022 Society of Chemical Industry.

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Section: Resultsmentioning

confidence: 99%

Section: Optimization Of the Isomerization-redox Cascadementioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Development of an enzymatic cascade to systematically utilize lignocellulosic monosaccharide

Tang

Chen

Yu³

et al. 2022

J Sci Food Agric

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“…Two plasmids of pET28a and pYES2 preserved by our laboratory were stored in E. coil DH5α. The OsRpiB gene with a double mutation of R94N/N137Q was generated from our previous study (Tang et al 2022). Based on the genome sequencing data, the genes of XK and XDH were ampli ed by PCR using the genome DNA of T. oedocephalis as the template.…”

Section: Recombinant Plasmids and Strains Constructionmentioning

confidence: 99%

“…In this study, a ribose-5-phosphate isomerase mutant from Ochrobactrum sp. (OsRpiB) with unique substrate scope was transformed into T. oedocephalis to endow it the xylose assimilation and xylitol production ability (Tang et al 2021(Tang et al , 2022. Then the xylulose kinase (XK) and the xylitol dehydrogenase (XDH) genes were overexpressed to further redirect the carbon ux to erythritol (Fig.…”

Section: Introductionmentioning

confidence: 99%

Construction of a xylose metabolic pathway in Trichosporonoides oedocephalis ATCC 16958 for the production of erythritol and xylitol

Deng,

Mu,

Chen

et al. 2023

Biotechnol Lett

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Erythritol is a valuable compound as sweetener and chemical material however cannot be fermented from the abundant substrate xylose. MethodsThe strain Trichosporonoides oedocephalis ATCC 16958 was employed to produce polyols including xylitol and erythritol by metabolic engineering approaches. ResultsThe introduction of a substrate selective ribose-5-phosphate isomerase endowed T. oedocephalis with xylose-assimilation activity to produce xylitol, and eliminated glycerol production simultaneously. A more value-added product, erythritol was produced by further introducing a homologous xylulose kinase. The carbon ux was redirected from xylitol to erythritol by adding high osmotic pressure. The production of erythritol was improved to 46.5 g/L in asks by fermentation optimization, and the process was scaled up in a 5-L fermentor, with 40 g/L erythritol production after 120 h, and a time-space yield of 0.56 g/L/h. ConclusionThis study demonstrated the potential of T. oedocephalis in the synthesis of multiple useful products from xylose.

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