Development of a thermostabilised triplex LAMP assay with dry-reagent four target lateral flow dipstick for detection of Entamoeba histolytica and non-pathogenic Entamoeba spp.

Foo, Phiaw Chong; Yean, Chan Yean; Mohamed, Maizan; Kin, Wong Weng; Najian, A.B. Nurul; Lim, Boon Huat

doi:10.1016/j.aca.2017.02.019

Cited by 41 publications

(38 citation statements)

References 25 publications

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“…This procedure has provided great help in diagnosing a multitude of RNA viruses [10] . Multiplexed LAMP assays were developed in order to detect multiple pathogens in the same test tube, with the use of more primers or with unique fluorescent signals [ 11 , 12 ]. All these iterations of the LAMP procedure show how promising it is in the virial diagnostic field.…”

Section: Working Principle Of Lamp Assay For Nucleic Acid Detectionmentioning

confidence: 99%

Mini review: Recent progress in RT-LAMP enabled COVID-19 detection

Thompson

Liu

2020

Sensors and Actuators Reports

174

179

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Section: Working Principle Of Lamp Assay For Nucleic Acid Detectionmentioning

confidence: 99%

Mini review: Recent progress in RT-LAMP enabled COVID-19 detection

Thompson

Liu

2020

Sensors and Actuators Reports

174

179

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“…Many possibilities have been developed to detect positive LAMP reactions, such as colourimetric detection using fluorescent dyes, UV light irradiation, agarose gel electrophoresis, turbidity, real-time fluorescence, smartphone, lateral flow assay (LFA), and AC susceptometry. The fluorescent dyes calcein, the SYBR Green dyes, and the EvaGreen are the most widely used for detecting LAMP amplicons [12,19,20]. The other dyes used for this purpose are malachite green dye, hydroxynaphthol blue dye, Goldview dye, GelRed dye, SYTO fluorescent dye, leuco crystal violet (LCV) dye, and berberine dye [19].…”

Section: Detection Of Lamp Productsmentioning

confidence: 99%

Loop-Mediated Isothermal Amplification (LAMP): The Better Sibling of PCR?

Soroкa

Wasowicz

Rymaszewska

2021

Cells

224

100

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In 1998, when the PCR technique was already popular, a Japanese company called Eiken Chemical Co., Ltd. designed a method known as the loop-mediated isothermal amplification of DNA (LAMP). The method can produce up to 109 copies of the amplified DNA within less than an hour. It is also highly specific due to the use of two to three pairs of primers (internal, external, and loop), which recognise up to eight specific locations on the DNA or RNA targets. Furthermore, the Bst DNA polymerase most used in LAMP shows a high strand displacement activity, which eliminates the DNA denaturation stage. One of the most significant advantages of LAMP is that it can be conducted at a stable temperature, for instance, in a dry block heater or an incubator. The products of LAMP can be detected much faster than in standard techniques, sometimes only requiring analysis with the naked eye. The following overview highlights the usefulness of LAMP and its effectiveness in various fields; it also considers the superiority of LAMP over PCR and presents RT-LAMP as a rapid diagnostic tool for SARS-CoV-2.

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“…Wang et al 15 reported a multiplex LAMP-LFD for the simultaneous detection of Staphylococcus aureus and Enterococcus faecalis by using FITC-and digoxigenin (Dig)-modified primers in the LAMP assay. Moreover, Foo et al 16 reported a multiplex LAMP-LFD for the detection of pathogenic Entamoeba histolytica and nonpathogenic Entamoeba spp.…”

mentioning

confidence: 99%

Development of Loop-Mediated Isothermal Amplification–Based Lateral Flow Device Method for the Detection of Malaria

Mallepaddi

Lai

Podha

et al. 2018

The American Journal of Tropical Medicine and Hygiene

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The present study aims to develop a method for rapid diagnosis of malaria using loop-mediated isothermal amplification (LAMP) combined with a lateral flow device (LFD). By adding the biotin-labeled and fluorescein amidite-labeled loop primers to the LAMP reaction solution, the end product can be visualized on a LFD. The entire procedure takes approximately 42 minutes to complete, LAMP assay exhibited high sensitivity, as the detection limit was 0.01 pg/μL for all five species. It was demonstrated that all ( = 90) and ( = 56) were positively amplified by LAMP-LFD assay, whereas healthy donor samples ( = 8) were negative. However, not all mixed infections were positive, and other infected nonmalaria samples were negative. Loop-mediated isothermal amplification-LFD represents a robust approach with potential suitability for use in resource-constrained laboratories. We believe that LAMP-LFD has a potential to be developed as point-of-care diagnostic tool in future.

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Development of a thermostabilised triplex LAMP assay with dry-reagent four target lateral flow dipstick for detection of Entamoeba histolytica and non-pathogenic Entamoeba spp.

Cited by 41 publications

References 25 publications

Mini review: Recent progress in RT-LAMP enabled COVID-19 detection

Mini review: Recent progress in RT-LAMP enabled COVID-19 detection

Loop-Mediated Isothermal Amplification (LAMP): The Better Sibling of PCR?

Development of Loop-Mediated Isothermal Amplification–Based Lateral Flow Device Method for the Detection of Malaria

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