Development of a three-dimensionally printed scaffold grafted with bone forming peptide-1 for enhanced bone regeneration with in vitro and in vivo evaluations

Lee, Sang Jin; Won, Jong‐Eun; Han, Changhak; Yin, Xiang Yun; Kim, Hyung Keun; Nah, Haram; Kwon, Il Keun; Min, Byoung‐Hyun; Kim, Chul‐Ho; Shin, Yoo Seob; Park, Su A

doi:10.1016/j.jcis.2018.12.097

Cited by 44 publications

(50 citation statements)

References 67 publications

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“…The scaffolds were then implanted in rabbit calvarial defect for enhanced vessel and bone regeneration. 79 Lin 2019 prepared PCL composite membrane containing 20 wt% cobalt-substituted hydroxyapatite (CoHA) powders by solvent casting for local release of cobalt ion to reduce infection and inflammation. Significantly increased cell proliferation (over 90% after 7 days of culture), calcium deposition, and good antibacterial effect was observed.…”

Section: Cellular Bioactivity On Pcl Scaffoldsmentioning

confidence: 99%

Polycaprolactone as biomaterial for bone scaffolds: Review of literature

Dwivedi

Kumar

Pandey

et al. 2020

Journal of Oral Biology and Craniofacial Research

500

290

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Bone tissue engineering using polymer based scaffolds have been studied a lot in last decades. Considering the qualities of all the polymers desired to be used as scaffolds, Polycaprolactone (PCL) polyester apart from being biocompatible and biodegradable qualifies to an appreciable level due its easy availability, cost efficacy and suitability for modification. Its adjustable physio-chemical state, biological properties and mechanical strength renders it to withstand physical, chemical and mechanical, insults without significant loss of its properties. This review aims to critically analyse the efficacy of PCL as a biomaterial for bone scaffolds. 1.1. Polymers as biomaterial for scaffolds The characteristics of a biomaterial that must be scrutinized thoroughly before considering it for bone tissue engineering applications includes chemical composition, biological and structural characteristics, degradation behavior and fabrication process. Polymeric scaffolds are of considerable interest due to their distinctive features, such

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Section: Cellular Bioactivity On Pcl Scaffoldsmentioning

confidence: 99%

Polycaprolactone as biomaterial for bone scaffolds: Review of literature

Dwivedi

Kumar

Pandey

et al. 2020

Journal of Oral Biology and Craniofacial Research

500

290

View full text Add to dashboard Cite

show abstract

“…Among various mesodermal differentiation capacities, osteogenesis is by far the most thoroughly studied functional phenotype of TMSCs . TMSCs cultured under osteogenic differentiation factors for 21 days increased the gene and protein expression levels of osteocalcin (OC) and alkaline phosphatase (ALP) as well as matrix mineralization as visualized by Alizarin red S staining .…”

Section: Tonsil‐derived Mscs (Tmscs)mentioning

confidence: 99%

“…It is intriguing to note that the osteogenic efficiency of TMSCs increases up to passage 10 and decreases thereafter . The osteogenicity of TMSCs was shown to be further enhanced in the presence of other factors such as fiber growth factor 5 and bone forming peptide 1 (BFP1) by regulating cellular transcriptional pathways such as extracellular‐signal‐regulated kinase 1/2, and bone morphogenetic protein–mitogen‐activated protein kinase, respectively. Recently, far‐infrared irradiation was reported to induce osteogenicity of TMSCs .…”

Section: Tonsil‐derived Mscs (Tmscs)mentioning

confidence: 99%

“…TMSCs have been tested in animal models of osteoporosis , osteoradinecrosis (ORN) , or calvarial defect and have demonstrated some promising outcomes as a feasible therapeutic treatment for bone‐related disorders (Table ). For example, tail injections of undifferentiated TMSCs or conditioned medium (CM) from TMSCs decreased the progression of osteoporosis in mice with BM depletion .…”

Section: Tonsil‐derived Mscs (Tmscs)mentioning

confidence: 99%

“…In a few studies, various biomaterials have been applied together with osteogenically differentiated TMSCs to create a more effective therapeutic application. Fabrication of TMSCs using a scaffold consisting of polycaprolcatone biomaterial and BFP1 increased cell adhesion and osteogenic differentiation thereby enhancing the bone regeneration of the calvarial defect region of rabbits . Other biomaterials made up with in situ crosslinkable gelatin‐hydroxyphenyl propionic acid hydrogel (GHH) , methacrylated chondroitin sulfate , or inorganic mesocrystals were also tested to fabricate the osteogenically differentiated TMSCs, and they promoted osteogenesis and bone mineralization in both in vitro and in vivo studies.…”

Section: Tonsil‐derived Mscs (Tmscs)mentioning

confidence: 99%

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Application of Tonsil-Derived Mesenchymal Stem Cells in Tissue Regeneration: Concise Review

Choi

Kim

et al. 2019

Stem Cells

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Since the discovery of stem cells and multipotency characteristics of mesenchymal stem cells (MSCs), there has been tremendous development in regenerative medicine. MSCs derived from bone marrow have been widely used in various research applications, yet there are limitations such as invasiveness of obtaining samples, low yield and proliferation rate, and questions regarding their practicality in clinical applications. Some have suggested that MSCs from other sources, specifically those derived from palatine tonsil tissues, that is, tonsil-derived MSCs (TMSCs), could be considered as a new potential therapeutic tool in regenerative medicine due to their superior proliferation rate and differentiation capabilities with low immunogenicity and ease of obtaining. Several studies have determined that TMSCs have differentiation potential not only into the mesodermal lineage but also into the endodermal as well as ectodermal lineages, expanding their potential usage and placing them as an appealing option to consider for future studies in regenerative medicine. In this review, the differentiation capacities of TMSCs and their therapeutic competencies from past studies are addressed. STEM CELLS 2019;37:1252-1260 SIGNIFICANCE STATEMENTMesenchymal stem cells (MSCs) are considered as a great candidate for tissue engineering in regenerative medicine. Tonsil-derived MSCs (TMSCs) could be an attractive option for clinical applications because of their noninvasiveness of tissue collection, relatively high proliferation rate, and low allogenicity. This review addresses potential differentiation capabilities of TMSCs into mesodermal, endodermal, and ectodermal lineages reported from previous in vitro and in vivo studies as well as their potential applications for treating various human diseases.

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Bone Microenvironment‐Mimetic Scaffolds with Hierarchical Microstructure for Enhanced Vascularization and Bone Regeneration

et al. 2022

Adv Funct Materials

117

111

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Microchannel networks within engineered 3D scaffold can allow nutrient exchange and rapid blood vessels formation. However, fabrication of a bone microenvironment‐mimicking scaffold with hierarchical micro/nanofibrous and microchannel structures is still a challenge. Herein, inspired by structural and functional cues of bone remodeling, a microchannel networks‐enriched nanofibrous scaffold by using 3D printing and thermally induced phase separation techniques, which can facilitate cells migration and nutrients transportation, is developed. The customizable vascular‐like structure of polycaprolactone within the nanofibrous gelatin‐silica scaffold is fabricated using 3D‐printed sacrificial templates, while dimethyloxalylglycine (DMOG)‐loaded mesoporous silica nanoparticles (MSNs) located on the scaffold surface and bone forming peptide‐1 (BFP)‐loaded MSNs embedded in the scaffold are implemented for sequential release of DMOG and BFP. The cell experiments show that dual‐drug delivery scaffold (DBM/GP) promotes angiogenesis by stimulating migration, tube formation, and angiogenesis‐related genes/protein expression of endothelial cells, and osteogenesis by promoting osteo‐related genes expression and mineral deposition of osteoblasts. Additionally, DBM/GP scaffold facilitates the angiogenic activity of osteoblasts by activating phosphatidylinositol 3‐kinase/protein kinase B/hypoxia inducible factor‐1α pathway. Furthermore, enhanced vascularization and bone regeneration of DBM/GP scaffold are demonstrated via subcutaneous and skull defect models. Overall, this study reveals that the bone microenvironment‐mimetic dual‐drug delivery scaffold provides a promising strategy for bone defects treatment.

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Development of a three-dimensionally printed scaffold grafted with bone forming peptide-1 for enhanced bone regeneration with in vitro and in vivo evaluations

Cited by 44 publications

References 67 publications

Polycaprolactone as biomaterial for bone scaffolds: Review of literature

Polycaprolactone as biomaterial for bone scaffolds: Review of literature

Application of Tonsil-Derived Mesenchymal Stem Cells in Tissue Regeneration: Concise Review

Bone Microenvironment‐Mimetic Scaffolds with Hierarchical Microstructure for Enhanced Vascularization and Bone Regeneration

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