Development of a Universal Fluorescent Probe for Gram‐Positive Bacteria

Kwon, Hyeokjin; Liu, Xiao; Choi, Eun Gyeong; Lee, Jung Yeol; Choi, Seongwook; Kim, Jun‐Young; Wang, Lu; Park, Sung‐Jin; Kim, Beomsue; Lee, Yong-An; Kim, Jong‐Jin; Kang, Nae Gyu; Chang, Young‐Tae

doi:10.1002/ange.201902537

Cited by 13 publications

(6 citation statements)

References 26 publications

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“…The colonies are 2.8 to 3.2 mm in diameter, circular, raised to slightly convex with smooth outline and smooth edges after 36 h incubation ( Figure 1A ). Gram staining was carried out ( Kwon et al, 2019 ) and the bacterium was Gram-negative ( Figure 1B ). SEM and TEM showed that the bacterial cells were in rod shape with 0.35–0.46 μm in width and 1.12–1.93 μm in length, and appeared singly or rarely in pairs ( Figures 1C , D ).…”

Section: Resultsmentioning

confidence: 99%

Growth and genome-based insights of Fe(III) reduction of the high-temperature and NaCl-tolerant Shewanella xiamenensis from Changqing oilfield of China

et al. 2022

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IntroductionA facultative anaerobe bacterium Shewanella xiamenensis CQ-Y1 was isolated from the wastewater of Changqing oilfield in Shaanxi Province of China. Shewanella is the important dissimilatory metal-reducing bacteria. It exhibited a well potential application in biodegradation and bioremediation.MethodsGenome sequencing, assembling and functional annotation were conducted to explore the genome information of CQ-Y1. The effect of temperatures and NaCl concentrations on the CQ-Y1 growth and Fe(III) reduction were investigated by UV visible spectrophotometry, SEM and XRD.ResultsGenomic analysis revealed its complete genome was a circular chromosome of 4,710,887 bp with a GC content of 46.50% and 4,110 CDSs genes, 86 tRNAs and 26 rRNAs. It contains genes encoding for Na+/H+ antiporter, K+/Cl− transporter, heat shock protein associated with NaCl and high-temperature resistance. The presence of genes related to flavin, Cytochrome c, siderophore, and other related proteins supported Fe(III) reduction. In addition, CQ-Y1 could survive at 10% NaCl (w/v) and 45°C, and temperature showed more pronounced effects than NaCl concentration on the bacterial growth. The maximum Fe(III) reduction ratio of CQ-Y1 reached 70.1% at 30°C without NaCl, and the reduction reaction remained active at 40°C with 3% NaCl (w/v). NaCl concentration was more effective than temperature on microbial Fe(III) reduction. And the reduction products under high temperature and high NaCl conditions were characterized as Fe3(PO4)2, FeCl2 and Fe(OH)2.DiscussionAccordingly, a Fe(III) reduction mechanism of CQ-Y1 mediated by Cytochrome c and flavin was hypothesised. These findings could provide information for a better understanding of the origin and evolution of genomic and metabolic diversity of S. xiamenensis.

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Section: Resultsmentioning

confidence: 99%

Growth and genome-based insights of Fe(III) reduction of the high-temperature and NaCl-tolerant Shewanella xiamenensis from Changqing oilfield of China

et al. 2022

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“…Furthermore, after incubation with red blood cells and bacteria, the probe could clearly identify E. coli and S. aureus without affecting red blood cells (Figure 4C). Meanwhile, Chang and co-workers developed BODIPY possessing boronic acid, probe 8, for the selective and sensitive detection of Gram-positive bacteria (Figure 5A) (Kwon et al, 2019). The boronic acid moiety can bind to the glycoprotein structure, which is expressed on the cell surface of Gram-positive bacteria.…”

Section: Interaction With Other Components In Bacterial Membranementioning

confidence: 99%

Strategies of Detecting Bacteria Using Fluorescence-Based Dyes

et al. 2021

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Identification of bacterial strains is critical for the theranostics of bacterial infections and the development of antibiotics. Many organic fluorescent probes have been developed to overcome the limitations of conventional detection methods. These probes can detect bacteria with “off-on” fluorescence change, which enables the real-time imaging and quantitative analysis of bacteria in vitro and in vivo. In this review, we outline recent advances in the development of fluorescence-based dyes capable of detecting bacteria. Detection strategies are described, including specific interactions with bacterial cell wall components, bacterial and intracellular enzyme reactions, and peptidoglycan synthesis reactions. These include theranostic probes that allow simultaneous bacterial detection and photodynamic antimicrobial effects. Some examples of other miscellaneous detections in bacteria have also been described. In addition, this review demonstrates the validation of these fluorescent probes using a variety of biological models such as gram-negative and -positive bacteria, antibiotic-resistant bacteria, infected cancer cells, tumor-bearing, and infected mice. Prospects for future research are outlined by presenting the importance of effective in vitro and in vivo detection of bacteria and development of antimicrobial agents.

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“…The development of chemical structures showing enhanced fluorescence emission upon recognition of molecular analytes is an active area of research in analytical chemistry and molecular imaging [1][2][3][4][5]. In addition to their utility as highly sensitive analytical probes for environmental (e.g., metal ion detection) or biological measurements (e.g., quantification of biomarkers in clinical samples) [6][7][8][9][10][11], they have been increasingly used to derivatise bioactive molecules (e.g., peptides, proteins, antibodies) and to prepare complex molecular constructs [12][13][14][15][16][17]. The resulting architectures combine good selectivity for receptors and/or cells of interest as well as high sensitivity derived from environmentally-sensitive readouts, and have been reported as useful tools for in vivo imaging of specific biological processes or subpopulations of cells [18][19][20][21][22][23][24].…”

Section: Introductionmentioning

confidence: 99%

Near-infrared benzodiazoles as small molecule environmentally-sensitive fluorophores

Moliner

Biazruchka

Konsewicz

et al. 2021

Front. Chem. Sci. Eng.

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The development of fluorophores emitting in the near-infrared spectral window has gained increased attention given their suitable features for biological imaging. In this work, we have optimised a general and straightforward synthetic approach to prepare a small library of near-infrared-emitting C-bridged nitrobenzodiazoles using commercial precursors. C-bridged benzodiazoles have low molecular weight and neutral character as important features that are not common in most near-infrared dyes. We have investigated their fluorescence response in the presence of a wide array of 60 different biomolecules and identified compound 3i as a potential chemosensor to discriminate between Fe2+ and Fe3+ ions in aqueous media.

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Development of a Universal Fluorescent Probe for Gram‐Positive Bacteria

Cited by 13 publications

References 26 publications

Growth and genome-based insights of Fe(III) reduction of the high-temperature and NaCl-tolerant Shewanella xiamenensis from Changqing oilfield of China

Growth and genome-based insights of Fe(III) reduction of the high-temperature and NaCl-tolerant Shewanella xiamenensis from Changqing oilfield of China

Strategies of Detecting Bacteria Using Fluorescence-Based Dyes

Near-infrared benzodiazoles as small molecule environmentally-sensitive fluorophores

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