Development of a useful single-reference HPLC method for therapeutic drug monitoring of phenytoin and carbamazepine in human plasma

We developed a reliable high-performance liquid chromatographic analysis method using a relative molar sensitivity (RMS) technique that does not require an authentic, identical reference analyte material to quantify blood serum carbamazepine, phenytoin, voriconazole, lamotrigine, meropenem, mycophenolic acid, linezolid, vancomycin, and caffeine levels for routine blood concentration measurements. Carbamazepine and caffeine were also used as non-analyte reference materials to calculate the RMS of each analyte. The RMS was calculated from the ratio of the slope of the calibration equation (analyte/non-analyte reference material), then used to quantify analytes in control serum samples spiked with carbamazepine, phenytoin, voriconazole, meropenem, mycophenolic acid, linezolid or vancomycin. In addition, the concentrations of these six drugs in control serum samples determined by the proposed RMS method agreed well with that obtained using a conventional method. The proposed RMS method is a promising tool for the clinical determination of nine drugs, given the accuracy, precision, and efficiency of quantifying these analytes.

show abstract

Estimation of Some Amino-Based Drugs in Human Blood Using HPLC Technique

Mezban Salih

2024

MOCA

View full text Add to dashboard Cite

Enantiomers in human plasma were analyzed using Solid Phase Extraction (SPE) and Chiral High- Performance Liquid Chromatography (HPLC). The chiral HPLC strategy allowed fractionation of DL, DD, LL, and LD varieties in human blood plasma with Targeted Solid Phase Extraction (TSPE). These methods are precise, economical, eco-friendly, and reproducible. Maximum plasma binding percentages for DL-, DD-, LL-, and LD-enantiomers were 63.39%, 56.79%, 84.11%, and 73.11%, respectively, at pH 9. Chiral resolution was strong, with separation factors over 1.0. Limits of Detection (LOD) ranged from 1 to 2.7 μg/mL, and Limits of Quantitation (LOQ) ranged from 5 to 10 μg/mL. The experimental data aligned well with the model, successfully applied to real plasma samples, enabling chiral monitoring of dipeptide stereoisomers in patients. HPLC, using an AmyCoat-RP column with an amylose polysaccharide stationary phase, proved effective for consistent and robust analysis, confirming the suitability of D-tryptophan, L-leucine, D-leucine, and L-tryptophan stereoisomers for clinical and research applications.

show abstract

scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.

Contact Info

customersupport@researchsolutions.com

10624 S. Eastern Ave., Ste. A-614

Henderson, NV 89052, USA

This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.

Blog Terms and Conditions API Terms Privacy Policy Contact Cookie Preferences Do Not Sell or Share My Personal Information

Made with 💙 for researchers

Part of the Research Solutions Family.

Development of a useful single-reference HPLC method for therapeutic drug monitoring of phenytoin and carbamazepine in human plasma

Cited by 4 publications

References 35 publications

Magnetic solid-phase extraction-based surface-enhanced Raman spectroscopy for label-free therapeutic drug monitoring of carbamazepine and clozapine in human serum

Magnetic solid-phase extraction-based surface-enhanced Raman spectroscopy for label-free therapeutic drug monitoring of carbamazepine and clozapine in human serum

Development of an HPLC method using relative molar sensitivity for the measurement of blood concentrations of nine pharmaceutical compounds

Estimation of Some Amino-Based Drugs in Human Blood Using HPLC Technique

Contact Info

Product

Resources

About