Development of a UV/Vis spectrophotometric method for analysis of total polyphenols from Caesalpinia peltophoroides Benth

Bueno, Fernanda; Machareth, Maria A. D.; Panizzon, Gean Pier; Lopes, Gisely Cristiny; Mello, João Carlos Palazzo de; Leite-Mello, Eneri Vieira de Souza

doi:10.1590/s0100-40422012000400031

Cited by 29 publications

(26 citation statements)

References 13 publications

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“…In this case, the specificity was evaluated by the absence of matrix effects, considering that natural products are complex matrices in which it is not possible to obtain a pure analyte. The absence of matrix effects is verified if the curves of linearity and specificity are parallel, in other words, if both curves show the same or very similar slopes (Blainski et al, 2013;Bueno et al, 2012;Ribani et al, 2004). The data obtained in the linearity and specificity tests for F2 and TG appear in Table 2 and are represented in Figure 1.…”

Section: Resultsmentioning

confidence: 83%

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Pharmaceutical topical gel containing proanthocyanidin polymers-rich fraction from Stryphnodendron adstringens

Costa¹,

Blainski²,

Kaplum³

et al. 2018

J. Med. Plants Res.

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The stem bark of Stryphnodendron adstringens, popularly known in Brazil as "barbatimão", has many biological activities, including antifungal activity. Considering the increasing interest of using "barbatimão" extract in the treatment of vaginal candidiasis, the aim of this study was to propose a pharmaceutical topical gel containing a proanthocyanidin polymers-rich fraction for use as a pharmacological agent in vaginal gels, as well as to evaluate the validation parameters for the determination of phenolic compounds. UV/Vis spectrophotometry was used as a quantitative method for quality control of topical gel and a proanthocyanidin polymers-rich fraction was used in this work. The proposed gel seems suitable for use in vaginal infections, and the analytical method was linear, specific, precise, accurate, reproducible and robust. This methodology complies with analytical application demands and it is easily performed in work routine.

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Section: Resultsmentioning

confidence: 83%

“…The analytical conditions for UV/Vis spectrophotometric method were evaluated as described by Blainski et al (2013) and Bueno et al (2012), and were confirmed with the use of pyrogallol, for 30 min and 760 nm, respectively; reference substance, time reaction and wavelength, as described in material and methods.…”

Section: Resultsmentioning

confidence: 99%

Pharmaceutical topical gel containing proanthocyanidin polymers-rich fraction from Stryphnodendron adstringens

Costa¹,

Blainski²,

Kaplum³

et al. 2018

J. Med. Plants Res.

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show abstract

“…A precisão é considerada uma das análises mais representativas, visto que revela o efeito das variações relacionadas aos dias, analistas ou equipamentos distintos, implicando garantia da reprodutibilidade do método (BUENO, 2012). A exatidão, por sua vez, reflete a proximidade dos resultados obtidos por uma metodologia analítica em relação aos valores verdadeiros (BRASIL, 2003).…”

Section: Alves Iabs Et Alunclassified

DESENVOLVIMENTO E VALIDAÇÃO DE METODOLOGIA ANALÍTICA POR ESPECTROFOTOMETRIA NO VISÍVEL PARA QUANTIFICAÇÃO DE TANINOS TOTAIS NA CASCA DO CAULE DE Simarouba amara Aubl.

et al. 2015

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RESUMO -As cascas do caule de Simarouba amara Aubl., Simaroubaceae, são amplamente utilizadas pela medicina popular brasileira e de vários países das Américas Central e do Sul para o tratamento de malária, parasitas intestinais, diarreia, anemia e febre. Apesar de os quassinoides serem considerados os marcadores quimiotaxonômicos da espécie, a indisponibilidade de padrões para comercialização tem sido um dos grandes obstáculos à realização de seu controle de qualidade. Diante disso, o propósito deste estudo foi o desenvolvimento e validação de metodologia analítica por espectrofotometria para a quantificação do teor de taninos totais das cascas do caule de S. amara, visto que esta classe de metabólitos é uma das mais abundantes e representativas já descritas para a espécie. O método proposto baseou-se na quantificação a 760 nm, após a adição do reagente Folin-Ciocalteu, e sua validação incluiu todos os parâmetros preconizados pela RE 899. Os resultados indicaram que o método proposto foi adequadamente desenvolvido e validado, constituindo ferramenta analítica útil para o controle de qualidade de S. amara.Palavras-chave: Reagente Folin-Ciocalteau; Simaroubaceae; Teor de Taninos. DEVELOPMENT AND VALIDATION OF ANALYTICAL METHODOLOGY BY SPECTROPHOTOMETRY IN THE VISIBLE FOR QUANTIFICATION OF TOTAL TANNINS IN THE STEM BARK OF Simarouba amara Aubl. ABSTRACT -The stem bark of

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“…The concentration of total Phenolics in the acetone leaf extracts of Sida cardifolia, Phyllanthus amarus and Adathoda vasica was determined by the Folin-Ciocalteu assay that involves reduction of the reagent by phenolic compounds, with concomitant formation of a blue complex, its intensity at 725nm increases linearly with the concentration of phenolics in the reaction medium 8 . The phenolic contents of the extracts were determined from calibration curve and were expressed in mg gallic acid equivalent/g of extract powder.…”

Section: Determination Of Total Phenolics By Folin-ciocalteau Assaymentioning

confidence: 99%

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2015

IJPSR

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Acetone extracts of the three medicinal plants Sida cardifolia, Phyllanthus amarus and Adathoda vasica were assessed for phytochemical components qualitatively and quantitatively and antioxidant activity. The results revealed that all the three contained alkaloids, carbohydrates, flavonoids, tannins, anthocyanins, steroids and phenols. Saponins were absent in all the three extracts. Sida cardifolia with 1.53mg and 7.09mg GA equivalent/g of extract powder showed maximum amount of Phenolics and flavonoids.The total sugars and proanthocyanidins was maximum in Phyllanthus amarus with 8.45ug glucose equivalent/g of extract powder and 10.84mg GA equivalent/g of extract powder. Tannin content was found to be high in Adathoda vasica with 11.07mg GA equivalent/g of extract powder. Sida cardifolia acetone leaf extract showed the highest reducing power activity, Free radical scavenging potential and inhibition of lipid peroxidation. INTRODUCTION: In a healthy body prooxidants and antioxidants maintain a ratio and a shift in this ratio towards prooxidants gives rise to oxidative stress. This oxidative stress may be either mild or severe depending on the extent of shift. This causes several diseases such as cardiovascular diseases, neurological diseases, renal diseases, diabetes, skin diseases, ageing, respiratory diseases and inflammatory problems 1, 2. Over 70 degenerative diseases are due to oxidative stress. These free radicals are produced from two important sources, cellular metabolism and environmental sources.

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Development of a UV/Vis spectrophotometric method for analysis of total polyphenols from Caesalpinia peltophoroides Benth

Cited by 29 publications

References 13 publications

Pharmaceutical topical gel containing proanthocyanidin polymers-rich fraction from Stryphnodendron adstringens

Pharmaceutical topical gel containing proanthocyanidin polymers-rich fraction from Stryphnodendron adstringens

DESENVOLVIMENTO E VALIDAÇÃO DE METODOLOGIA ANALÍTICA POR ESPECTROFOTOMETRIA NO VISÍVEL PARA QUANTIFICAÇÃO DE TANINOS TOTAIS NA CASCA DO CAULE DE Simarouba amara Aubl.

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