Development of a Visual Method to Quantify the Gossypol Content in Cotton Seeds

Benbouza, Halima; Lognay, Georges; Palm, Rodolphe; Baudoin, Jean-Pierre; Mergeai, Guy

doi:10.2135/cropsci2002.1937

Cited by 19 publications

(16 citation statements)

References 14 publications

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“…However, undoubtedly such difference does not restrict the utilization of G. hirsutum hairy root cultures as an effective alternative source for gossypol as it requires only 21-28 days of culture as compared to longer time required for growing the plant to maturity in field. The gossypol content in the seed and other organs is closely related to the overall glandulosity and the genotype [12,64,65]. The total gossypol content of seeds of different genotypes varied from as low as 0.2 ± 0.05 mg/g DW to as high as 64 ± 1.5 mg/g DW [62,66].…”

Section: Discussionmentioning

confidence: 99%

Efficient Production of Gossypol from Hairy Root Cultures of Cotton (Gossypium hirsutum L.)

Verma

Trivedi²,

Singh³

et al. 2009

CPB

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A protocol for induction and establishment of Agrobacterium rhizogenes mediated hairy root culture of Gossypium hirsutum was developed through infection with the A4 strain and co-cultivation on hormone-free semi-solid MS medium with B5 vitamins. It resulted in the emergence of hairy roots from the leaf explants, 21 days after infection. The transformation of hairy roots was established by PCR amplification of rol B and rol C genes of the Ri plasmid. All root lines expressed gossypol, although distinct inter-clonal quantitative variations were noticed. Five independent hairy root lines were studied for their growth kinetics as well as gossypol production. The yield potentials of one of them superseded others, as well as the non-transformed, in-vitro grown control roots. The content of gossypol in hairy roots reached a level of 2.43 mg/g DW. It was 4.5 times higher than in vitro and 1.47 times higher than in vivo grown roots of G. hirsutum. Selection of high gossypol producing hairy root lines of G. hirsutum can provide an alternative source for large-scale production of gossypol.

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Section: Discussionmentioning

confidence: 99%

Efficient Production of Gossypol from Hairy Root Cultures of Cotton (Gossypium hirsutum L.)

Verma

Trivedi²,

Singh³

et al. 2009

CPB

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“…É capaz de proteger a planta contra danos provocados por insetos, mas também é tóxico para os animais, embora em menor nível para os ruminantes (ARIELI, 1998;BENBOUZA et al, 2002;BLACKWELDER et al, 1998;LANHAM;HORNER, 1987;RANDEL;WYSE, 1992;RISCO et al, 2002;ROGÉRIO et al, 2003;SANTOS, 1997).…”

Section: O Gossipolunclassified

Efeito da dieta contendo caroço de algodão no desempenho, características quantitativas da carcaça e qualitativas da carne de novilhos Nelore confinados

Pesce¹

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Efeito da dieta contendo caroço de algodão no desempenho, características quantitativas da carcaça e qualitativas da carne de novilhos Nelore confinados. The purpose of this work was to evaluate performance, quantitative characteristics of the carcass and qualitative characteristics of the meat of confined castrated Nelore males. Animals were confined with four diets containing 80% concentrate (dry matter): a control diet devoid of whole cottonseed (CA0), a diet containing 10%(CA10) or 20% of whole cottonseed (CA20) and a diet containing 20% of whole cottonseed heated in a climate chamber (CA20H). At the end of the experimental period, there were no differences between treatments for dry matter intake. However, the average daily gain, live weight and feed efficiency were greater for animals fed the whole cottonseed-enriched diets, whit no difference among diets when compared to control. Values documented for blood urea-N were significantly higher for treatments containing whole cottonseed, while glucose and total serum protein did not differ. Carcass yield, loin eye area, subcutaneous fat thickness, renal and pelvic fat, warm carcass weight and cold carcass weight, weight of the liver and values of carcass pH were not significantly different. Differences were noted only for carcass temperature 1 hour and 24 hours after slaughter, lower for CA0 treatment. There were no significant differences for dry matter content and ethereal meat extract. No differences were found between total cooking losses and meat tenderness between 7 and 14 days of maturing, as verified by shearing forces. Only time effect was noted, with meat matured for 14 days presenting with increased tenderness and less total cooking losses. Sensorial analysis of the Longissimus dorsi did not show differences among treatments for typical odor, juicyness and typical or off-flavour. No statistical difference was found for total saturated fatty acids and omega-3, or the relations between total unsaturated or monounsaturated and saturated fatty acids. However, animals fed whole cottonseed-enriched diets had higher concentrations of polyunsaturated fatty acids and omega-6 and lower concentrations of monounsaturated fatty acids in the Longissimus dorsi. Whole cottonseed can be

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“…The HPLC method optimized by Benbouza et al (2002) was used to quantify the gossypol content on single seed samples. After being peeled, cut and weighed the seeds were ground in graduated 20 ml tubes with 10-15 ml of liquid nitrogen and 0.5-1 ml of glacial acetic acid.…”

Section: Assessment Of Seed By Seed Gossypol Contentmentioning

confidence: 99%

Expression of the “glanded-plant and glandless-seed” trait of Australian diploid cottons in different genetic backgrounds

et al. 2008

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The expression of the "glanded-plant and glandless-seed" trait was assessed using High Performance Liquid Chromatography (HPLC) analysis methods in different Gossypium hybrids obtained by crossing Australian diploid cottons and various diploid and tetraploid species. Significant variation in the gossypol content in the seed was observed among the analyzed genotypes. HPLC data demonstrated that the gossypol synthesis repression mechanism in the Australian diploid species belonging to C and G genomes was dominant but did not confirm its preferential functioning against A genome species bearing GL 2 locus. About 10% of the produced seeds had total gossypol content lower than the limit imposed by the World Health Organisation (600 ppm) for the use of cotton flour in food and feed. HPLC analysis of the terpenoids aldehyde (TA) contents in the aerial parts of the hybrids showed important qualitative and quantitative variability. This result could indicate a certain separation between pigment gland morphogenesis and terpenoid synthesis mechanisms in cotton.

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Development of a Visual Method to Quantify the Gossypol Content in Cotton Seeds

Cited by 19 publications

References 14 publications

Efficient Production of Gossypol from Hairy Root Cultures of Cotton (Gossypium hirsutum L.)

Efficient Production of Gossypol from Hairy Root Cultures of Cotton (Gossypium hirsutum L.)

Efeito da dieta contendo caroço de algodão no desempenho, características quantitativas da carcaça e qualitativas da carne de novilhos Nelore confinados

Expression of the “glanded-plant and glandless-seed” trait of Australian diploid cottons in different genetic backgrounds

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