Development of a Water‐Dispersible Supramolecular Complex of Polyphenol with Polypeptides for Attenuation of the Allergic Response using a Mechanochemical Strategy

Abstract

The prevalence of allergic disorders has increased worldwide in recent decades. Polyphenols, including resveratrol and curcumin, are posited to have potential as therapeutic agents for allergy; however, their use has been limited by poor water solubility. Accordingly, a highly concentrated, water dispersible, supramolecular complexes of polyphenols with polypeptides (poly‐L‐lysine, poly‐γ‐glutamic acid) and gelatin using high‐speed vibration milling are developed. The complex exhibits resistance to photobleaching and thermal radiation. Treatment of a rat basophilic leukemia cell line (RBL‐2H3) with polypeptide complexes containing resveratrol is suppressed allergic responses in vitro. Moreover, aerosolized administration of polypeptide complexes demonstrates excellent bioavailability and inhibition of immediate hypersensitivity reactions in ear tissue in vivo. Furthermore, the method avoids the use of organic solvent and therefore reduces undesirable biological responses.

Extracellular Vesicles Comprising Carborane Prepared by a Host Exchanging Reaction as a Boron Carrier for Boron Neutron Capture Therapy

“…As representative peaks from pyrene in 1 H NMR disappeared at 24 h incubation after mixing the pyrene/HP-β-CDx complex and the UV−vis spectra of pyrene got broadened, and the loading of pyrene was achieved (Figure S5). Since the ratio of fluorescence intensities of pyrene at 375 (FI 375 ) and 385 nm (FI 385 ) is used as an indicator of polarity of the microenvironment, 90 we measured the fluorescence spectra of the pyrene/ HP-β-CDx complex and pyrene@EVs. The ratios of FI 375 /FI 385 of the pyrene/HP-β-CDx complex and pyrene@EVs were determined to be 0.92 and 0.90, respectively, indicating that the pyrenes were transferred from the hydrophobic cavity of HP-β-CDx to a more hydrophobic environment like a lipid membrane via an exchanging reaction.…”