Strain gauges are often used to measure vertical wheel loads in a railroad track. This approach is based on the concept of differential-shear-strain (DSS) measurement: The difference in vertical shear force between two points along a beam equals the magnitude of the resultant of applied vertical forces in between. With a slight modification to the strain-gauge positions and installation of an additional set of strain gauges, this concept can be extended to measure the vertical rail–tie interface reaction forces, thus assessing the tie support conditions. Although the application of DSS measurements for vertical-wheel-load quantification is widely prevalent in the railroad community, the validity of this approach for tie reaction measurement has been relatively unexplored. Conceptually, the approach is similar to the vertical-wheel-load measurement system, with the only difference being the placement of the strain gauges along the rail. Nevertheless, several questions have been raised about how different track and loading configurations can affect the accuracy of such a measurement system. To address some of these concerns and establish this approach as a viable method for tie support condition assessment, a field-validation effort was undertaken. Under the scope of this research effort, the strain-gauge-based DSS measurement system for rail–tie interface reaction-force measurement was evaluated in the field under static as well as dynamic loading. The study showed that the strain-gauge-based measurement approach is as accurate as other conventional methods of tie reaction-force measurement.