Development of a widely targeted volatilomics method for profiling volatilomes in plants

Yuan, Honglun; Cao, Guangping; Huang, Xiaodong; Huang, Menglan; Du, Pengmeng; Tan, Tingting; Zhang, Youjin; Zhou, Haihong; Liu, Xianqing; Liu, Ling; Jiangfang, Yiding; Li, Yufei; Liu, Zhenhuan; Fang, Chuanying; Zhao, Lingyun; Fernie, Alisdair R.; Luo, Jie

doi:10.1016/j.molp.2021.09.003

Cited by 99 publications

(41 citation statements)

References 66 publications

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“…The sample was gently mixed and sonicated for 5 min. Samples were preheated for 10 min at 50 °C and extracted for 20 min at 50 °C before GC-MS analysis [ 70 ].…”

Section: Methodsmentioning

confidence: 99%

Comparative Metabolomics Reveals Key Determinants in the Flavor and Nutritional Value of Coconut by HS-SPME/GC-MS and UHPLC-MS/MS

Guo

Lai

et al. 2022

Metabolites

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Coconut is a tropical fruit whose flesh has high flavor quality and nutritional value; however, the differences between coconut varieties are still unclear. Here, volatiles and non-volatiles were profiled at three ripening stages by HS-SPME/GC-MS and UHPLC-MS/MS in two coconut varieties (Hainan Tall, HT and Green Dwarf, GD). Four metabolite classes of volatiles were associated with good aroma including hydrocarbons, benzenoids, alcohols and esters, and these volatiles were generally higher in GD, especially at 7 and 9 months of coconut growth. Pathway-based metabolomics revealed that flavonols and their derivatives were significantly enriched in HT, and some of these metabolites were key determinants of HT flesh bitterness, including kaempferol 7-O-glucoside, a known bitter metabolite. Despite the overall accumulation of amino acids, including L-alanine, L-serine and L-methionine in GD, comparative metabolomics revealed that HT flesh provides a higher content of vitamins than GD. This study sheds light on the metabolic pathways and key metabolites differentiating the flesh flavor quality and nutritional value among coconut varieties, and reveals the possible mechanisms of flavor formation and regulation in coconut fruits.

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“…The sample was gently mixed and sonicated for 5 min. Samples were preheated for 10 min at 50 °C and extracted for 20 min at 50 °C before GC-MS analysis [ 70 ].…”

Section: Methodsmentioning

confidence: 99%

Comparative Metabolomics Reveals Key Determinants in the Flavor and Nutritional Value of Coconut by HS-SPME/GC-MS and UHPLC-MS/MS

Guo

Lai

et al. 2022

Metabolites

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“…Each compound matched a quantitative ion and 2-3 qualitative ions. The RT of the detected chromatographic peak was consistent with the standard reference, and all the selected ions appeared in the quality spectrum of the sample after deducting the background, then the substance was determined (Yuan et al, 2022). Quantitative ions were selected to integrate and correct chromatographic peaks to enhance the accuracy of quantification.…”

Section: Qualitative and Quantitative Metabolitementioning

confidence: 99%

Metabolomics Analysis Reveals the Differences Between Bupleurum chinense DC. and Bupleurum scorzonerifolium Willd.

et al. 2022

Front. Plant Sci.

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Bupleurum chinense DC. and Bupleurum scorzonerifolium Willd. are two varieties of Bupleuri Radix in Chinese Pharmacopoeia 2020. The clinical efficacy of the two bupleurum species is different. The difference in clinical efficacy is closely related to the composition of plant metabolites. In order to analyze the difference in metabolites, we used liquid chromatography coupled with mass spectrometry (LC-MS) for untargeted metabolome and gas chromatography coupled with mass spectrometry (GC-MS) for widely targeted metabolome to detect the roots (R), stems (S), leaves (L), and flowers (F) of two varieties, and detected 1,818 metabolites in 25 classes. We performed a statistical analysis of metabolites. Differential metabolites were screened by fold-change and variable importance in the projection values of the OPLS-DA model, and significant differences were found among different groups. The content of active components (triterpenoid saponins) was found to be high in the BcR group than in the BsR group. Other pharmacological metabolites were significantly different. By Kyoto Encyclopedia of Genes and Genomes annotation and enrichment analysis, we found that differential metabolites of the aboveground parts mainly concentrated in monoterpenoid biosynthesis, while the differential metabolites of the root mainly concentrated in sesquiterpenoid and triterpenoid biosynthesis. Differences in metabolic networks may indirectly affect the metabolic profile of Bc and Bs, leading to differences in clinical efficacy. Our study provides a scientific basis for subsequent biosynthesis pathway and related bioactivity research, and provides a reference for developing non-medicinal parts and guiding the clinical application of Bupleuri Radix.

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“…Sample extraction of the widely targeted metabolome for LC-MS and GC-MS analyses has been described previously (Chen et al, 2013;Yuan et al, 2022). The detailed methods are listed in Supplementary file 1.…”

Section: Metabolite Extraction and Analysismentioning

confidence: 99%

“…Specific ion-detection modes were used for accurate scanning, including the scanning typical RTs and qualitative and quantitative ions. As previously described, one quantitative ion and two-three qualitative ions were selected for each compound (Yuan et al, 2022). Quantitative ions were selected to integrate and correct the peak areas for further data processing.…”

Section: Metabolite Extraction and Analysismentioning

confidence: 99%

Identification and characterization of a novel gene involved in glandular trichome development in Nepeta tenuifolia

et al. 2022

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Nepeta tenuifolia is a medicinal plant rich in terpenoids and flavonoids with antiviral, immunoregulatory, and anti-inflammatory activities. The peltate glandular trichome (PGT) is a multicellular structure considered to be the primary storage organ for monoterpenes; it may serve as an ideal model for studying cell differentiation and the development of glandular trichomes (GTs). The genes that regulate the development of GTs have not yet been well studied. In this study, we identified NtMIXTA1, a GT development-associated gene from the R2R3 MYB SBG9 family. NtMIXTA1 overexpression in tobacco resulted in the production of longer and denser GTs. Virus-induced gene silencing of NtMIXTA1 resulted in lower PGT density, a significant reduction in monoterpene concentration, and the decreased expression of genes related to monoterpene biosynthesis. Comparative transcriptome and widely targeted metabolic analyses revealed that silencing NtMIXTA1 significantly influenced the expression of genes, and the production of metabolites involved in the biosynthesis of terpenoids, flavonoids, and lipids. This study provides a solid foundation describing a mechanism underlying the regulation of GT development. In addition, this study further deepens our understanding of the regulatory networks involved in GT development and GT development-associated metabolite flux, as well as provides valuable reference data for studying plants with a high medicinal value without genetic transformation.

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Development of a widely targeted volatilomics method for profiling volatilomes in plants

Cited by 99 publications

References 66 publications

Comparative Metabolomics Reveals Key Determinants in the Flavor and Nutritional Value of Coconut by HS-SPME/GC-MS and UHPLC-MS/MS

Comparative Metabolomics Reveals Key Determinants in the Flavor and Nutritional Value of Coconut by HS-SPME/GC-MS and UHPLC-MS/MS

Metabolomics Analysis Reveals the Differences Between Bupleurum chinense DC. and Bupleurum scorzonerifolium Willd.

Identification and characterization of a novel gene involved in glandular trichome development in Nepeta tenuifolia

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