Development of a zebrafish 4-day embryo-larval bioassay to assess toxicity of chemicals

Fraysse, Benoît; Mons, R.; Garric, Jeanne

doi:10.1016/j.ecoenv.2004.10.015

Cited by 285 publications

(168 citation statements)

References 40 publications

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“…1). Our study is supported by earlier findings on zebrafish exposed to chlorpyrifos (Sreedevi et al, 2014;Yu et al, 2015), cartap (Zhou et al, 2009), malathion (Fraysse et al, 2006), cypermethrin (Shi et al, 2011) bifenthrin (Jin et al, 2010), fipronil (Stehr et al, 2006) and acetofenate (Xu et al, 2008). …”

Section: Discussionsupporting

confidence: 93%

Effects of insecticides on aquatic ecosystems in Bangladesh

Sumon

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Section: Discussionsupporting

confidence: 93%

Effects of insecticides on aquatic ecosystems in Bangladesh

Sumon

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“…It also demonstrates that the larval stage of the olive flounder would be a good indicator organism for Aroclor 1254 contamination in coastal waters, and that the response recorded may be typical for other contaminants (Fraysse et al, 2006). Finally, exposure to Arolcor 1254 concentrations as low as 1…”

Section: Discussionmentioning

confidence: 75%

Toxicological Efects of Aroclor 1254 on the Embryonic Development of the Olive Flounder Paralichthys olivaceus

Min¹,

Kang²

2013

Fisheries and aquatic sciences

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We investigated the toxicological effects of Aroclor 1254 on the fertilized eggs, embryos and larvae of the olive flounder Paralichthys olivaceus. The survival rate and hatching success of the embryos decreased significantly in treated groups in an Aroclor 1254-dose-dependent manner. Significant differences were found at ≥5 µg/L Aroclor 1254 compared to the control group. Hatching success occurred at ≤10 µg/L Aroclor 1254, which was not significantly different to the control. Embryo malformation increased significantly at ≥1 µg/L, and included yolk-sac and tail-flexure abnormalities. There was a significant decrease in the survival rate of the larvae at ≥5 µg/L, which was accompanied by the malformations described above. Notably, concentrations as low as 1 µg/L caused a significant increase in abnormalities in the larvae, including incidences of multi-focal hemorrhages, pericardial and yolk-sac edema, inhibition of swim bladder inflation and severe developmental delay. The responses to Aroclor 1254-induced toxicity were generally similar among fertilized eggs, embryos and larvae from three separate flounder hatcheries: Cheju Island, Yeosu and Chungnam, South Korea. These results indicate the high acute toxicity of Arolcor 1254 concentrations of which as low as 1 μg/L in olive flounder larvae can affect unhatched embryos. To conclude, the average LC 50 values for Aroclor 1254 in the embryos and larvae were 50.92 and 3.08 μg/L, respectively. Additionally, the average EC 50 values, based on the rate of damage were 14.72 and 5.61 μg/L, respectively.

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“…Actual norms and guidelines are optimized in regard to economic advisements [24]. Reducing time and equipment-dependent costs (including the number of organisms) when estimating a concentrationeffect relationship seems to be more honoured than safely protecting the environment [25]. An example for a questionable proceeding is designing an experiment with high concentrations causing high effects and extrapolating the obtained data to the low effect situation.…”

Section: Changes In Experimental Design As a First Stepmentioning

confidence: 99%

Optimal test design for binary response data: the example of the fish embryo toxicity test

Keddig¹,

Schubert²,

Wosniok

2015

Environ Sci Eur

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Background: The fish embryo toxicity test (FET) is an established method in toxicology research for quantifying the risk potential of environmental contaminations and other substances. The typical results of the method are the half maximal effective concentration (EC 50 ) or the no observed effect concentration (NOEC). However, from an environmental perspective, it is most important to safely identify the concentration of the substance effect which lies above the effect under control condition (spontaneous effect). The common FET is not optimal to detect ECs for small target effects. This paper shows how to optimize the efficiency and consequently the benefit of the FET for small effects using an adequate experimental design. The approach presented here can be carried over to all test systems generating binary (yes/no) outcomes. Results: The experimental design has three components in this context: determination of spontaneous response, sample size calculation, and dose allocation. A strategy for all three components is proposed from which a design is given including precision requirements and makes the most effective use of the experimental effort. This strategy amounts to expanding the usual FET guidelines of Organisation for Economic Co-operation and Development, German Institute for Standardization, or American Society for Testing and Materials by adding a planning step that adapts the test to the specific user's need. Conclusions: For the practical calculation of an adapted design, a newly developed software is presented as R package toxtestD. It provides a user-friendly way of developing an optimal experimental design for the FET without in-depth statistical knowledge. The programme is suited for all experimental problems involving a binary outcome and a continuous concentration.

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Development of a zebrafish 4-day embryo-larval bioassay to assess toxicity of chemicals

Cited by 285 publications

References 40 publications

Effects of insecticides on aquatic ecosystems in Bangladesh

Effects of insecticides on aquatic ecosystems in Bangladesh

Toxicological Efects of Aroclor 1254 on the Embryonic Development of the Olive Flounder Paralichthys olivaceus

Optimal test design for binary response data: the example of the fish embryo toxicity test

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