Development of a μ-turbulent flow chromatography focus mode method for drug quantitation in discovery bioanalysis

Turnpenny, Paul; Fraier, Daniela; Chassaing, Christophe; Duckworth, Jonathan

doi:10.1016/j.jchromb.2007.05.032

Cited by 16 publications

(11 citation statements)

References 27 publications

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“…These other methods utilize a sophisticated column switching system involving another six port valve with a T-rotor 10–12,15,18. The same holds true for many other recently described fast online-SPE-LC-MS/MS methods which utilize special systems including the application of disposable SPE columns 23,26,44.…”

Section: Discussionmentioning

confidence: 92%

“…However, TFC is most widely used in online-SPE-LC-ESI-MS/MS for the analysis of drugs in biological samples 1–3. In the last three years, numerous methods have been published utilizing TFC in online-SPE-LC-ESI-MS/MS designed for the fast analysis of single drug compounds in biological samples, specifically plasma 10–17. Few approaches combine TFC with the high chromatographic resolution of modern sub-2 μm particle filled columns.…”

Section: Introductionmentioning

confidence: 99%

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Development of an ultra fast online-solid phase extraction (SPE) liquid chromatography electrospray tandem mass spectrometry (LC-ESI-MS/MS) based approach for the determination of drugs in pharmacokinetic studies

et al. 2011

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High-throughput analyses of a large number of samples for pharmacokinetic (PK) studies are essential in drug development. Analysis of drug candidates from blood using LC-ESI-MS generally requires separation of the plasma fraction followed by various offline sample preparation procedures. This step is a bottleneck that impedes throughput. In order to overcome this difficulty and accelerate analysis in PK and other studies, we developed an approach allowing the direct analysis of low volumes of whole blood (10 μL) after dilution and centrifugation. Samples were injected in an online-SPE-LC-ESI-MS/MS setup allowing a total run time of only 126 s for a full gradient separation. Analytes were extracted from the matrix within 30 s by turbulent flow chromatography. Subsequently, a full gradient separation was carried out within 1.5 minutes on a 50 × 2.1 mm (1.7 μm) RP-18 column and the analytes were sensitively detected by ESI-MS/MS in SRM mode. The performance of this new ultra fast online SPE-LC-ESI-MS/MS approach was demonstrated by the analysis of diclofenac (DCF), a widely used anti-inflammatory drug. DCF eluted at stable retention times (±0.33%) with narrow peak width (FWHM 3.3 ± 0.15 s). The method displays excellent analytical performance, with a limit of detection of 6 fmol on column, a linear range of over four orders of magnitude and a negligible carry over of 0.12 ± 0.03% for DCF. The PK profile of DCF administered by topical and intraperitoneal routes in rats and by oral route in one human volunteer is investigated using this method. Finally, general applicability of the approach for drugs is demonstrated by analysis of rofecoxib and several inhibitors of the soluble epoxide hydrolase. This new method requires only readily available, off the shelf standard LC instrumentation, and is compliant with the requirements of green analytical chemistry.

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Section: Discussionmentioning

confidence: 92%

Section: Introductionmentioning

confidence: 99%

Development of an ultra fast online-solid phase extraction (SPE) liquid chromatography electrospray tandem mass spectrometry (LC-ESI-MS/MS) based approach for the determination of drugs in pharmacokinetic studies

et al. 2011

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“…41,42 This is due to improved mass transfer across the bulk mobile phase that occurs as result of the high flow rates used which leads to 'turbulent flow,' thus enabling molecules to improve their radial distribution. This represents a potentially interesting alternative for sample analysis in metabonomics because of the combination of greatly reduced sample handling and relative ease of automation.…”

Section: Plasma/serummentioning

confidence: 99%

Sampling and Sample Preparation for LC-MS-Based Metabonomics/Metabolomics of Samples of Mammalian Origin

Wilson¹,

Michopoulos²,

Theodoridis³

2012

Comprehensive Sampling and Sample Preparation

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“…TFC columns marketed by Cohesive Technologies are widely used for on-line extraction of small molecules in biological matrix [23][24][25][26][27]. A TurboFlow HTLC C 18 column (0.5 mm × 50 mm; 50 m; 100 Å) was evaluated for its effectiveness and feasibility for use as the extraction column for the selected 11 therapeutic peptides.…”

Section: Column Selectionmentioning

confidence: 99%

Investigation of an on-line two-dimensional chromatographic approach for peptide analysis in plasma by LC–MS–MS

Liu

Tweed

Wujcik

2009

Journal of Chromatography B

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Development of a μ-turbulent flow chromatography focus mode method for drug quantitation in discovery bioanalysis

Cited by 16 publications

References 27 publications

Development of an ultra fast online-solid phase extraction (SPE) liquid chromatography electrospray tandem mass spectrometry (LC-ESI-MS/MS) based approach for the determination of drugs in pharmacokinetic studies

Development of an ultra fast online-solid phase extraction (SPE) liquid chromatography electrospray tandem mass spectrometry (LC-ESI-MS/MS) based approach for the determination of drugs in pharmacokinetic studies

Sampling and Sample Preparation for LC-MS-Based Metabonomics/Metabolomics of Samples of Mammalian Origin

Investigation of an on-line two-dimensional chromatographic approach for peptide analysis in plasma by LC–MS–MS

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