Development of Abiotic Stress Resistant Grapevine Varieties

Singh, Sanjay K.; Pradhan, S.; KRISHNA, HARE; Alizadeh, M.; Kumar, Chavlesh; Singh, Niranjan; Jadhav, Amol K.; Ramajayam, D.; Dev, Rahul; Singh, Rakesh

doi:10.1007/978-3-031-09875-8_4

Cited by 3 publications

(3 citation statements)

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“…These results agree with Valizadeh Kaji et al, 2013;Singh & Patel, 2016;Mulaei et al, 2020 andSingh et al, 2022 who used MS medium for plant culture initiation. Further, a half-MS has a more influential role in the establishment stage than full-MS strength, as Singh et al (2010) reported by using a half-strength MS medium for meristem cultured. This may result due to nutrient media components that are more suitable for pomegranate species and cultivars and offers suitable demands for the initiation stage.…”

Section: Discussionmentioning

confidence: 76%

“…Sodium hypochlorite and ethanol exhibit a successful effect in surface sterilization, which is the most commonly used as surface sterilant; other chemicals have also been tried successfully (Singh et al, 2010 andPrajwala et al, 2021). Many researchers have recommended using mercuric chloride (HgCl2) for surface disinfestation, which records a good survival percentage for the explants in pomegranate, in addition to their high effectiveness in totally removing bacteria and fungi from the explants.…”

Section: Discussionmentioning

confidence: 99%

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Worthy Micropropagation Protocols of Seven Cultivars of Pomegranate (Punica Granatum L.) Cultivars in the Province of Duhok, Kurd Istan Rigion of Iraq.

Esho,

Danial

2023

SJUOZ

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An efficient attempt was conducted to increase the mass production of seven local cultivars of pomegranate (Punica granatum L.) Masafik, Melisse, Radisho, Armishte, Diala, Halapja, and Dwarf pomegranate cultured in the Kurdistan region of Iraq from October 2021 to February 2023. Micropropagation technique was applied using eight combinations of plant growth regulators (PGRs) added to two media (MS and WPM). The results indicated that immersing explants in 70% Ethyl alcohol (EtOH) for (2 minutes) followed by immersion in (2.5%) Sodium Hypochlorite (NaOCl) plus 3 drops of tween 20 for 13 mins. was the more suitable combination for explant sterilization. MS and WPM media salts supplemented together with eight combinations of PGRs were carried out to evaluate the more suitable combination for the initiation stage. The obtained data clarified that the ½ strength of the MS medium (1/2 MS) supplemented with 0.5 BAP plus 0.5 mg/l Kinetin increases the pomegranate explant initiation response percentage. WPM basal medium was the preferred media to be used in the multiplication stage. The impact of different levels and protocols of Cytokinins, Auxins, and Gibberellins were investigated by eight protocols including using BAP, GA3, and adenine sulfate. The outcomes indicated that adding both concentrations of BAP (0.5 and 1.0 mg/l) separately supported by GA3 and adenine sulfate in amounts of (0.3 and 30 mg/l) respectively again were the most suitable protocol that affected the multiplication of regenerated shoots when compared to using BAP alone. Valuable results were found in rooting response after 6 weeks by using ½ strength of MS media along with two levels of Auxins (NAA, and IBA). Moreover, the visual observation records that around 85-90% of rooted cultures were successfully acclimatized by transferring the cultures from in vitro to the ex-vitro environment. Finally, Dwarf cultivars exceed all the other cultivars in studded parameters.

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Section: Discussionmentioning

confidence: 76%

Section: Discussionmentioning

confidence: 99%

Worthy Micropropagation Protocols of Seven Cultivars of Pomegranate (Punica Granatum L.) Cultivars in the Province of Duhok, Kurd Istan Rigion of Iraq.

Esho,

Danial

2023

SJUOZ

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“…Popular grape rootstocks have a narrow genetic base derived from a few American grape species (Riaz et al, 2019). V. parviflora Roxb., a Himalayan wild grape species, possesses desirable traits such as moderate vigor, drought resistance, and multiple disease resistance, making it a promising candidate as a rootstock (Singh et al, 2022). Combining the traits of this wild species with those of other grape species may result in rootstock tolerant to abiotic and biotic stresses with wider adaptability.…”

Section: Introductionmentioning

confidence: 99%

Studies on pollen micro-morphology, pollen storage methods, and cross-compatibility among grape (Vitis spp.) genotypes

Rane,

Thakre,

Verma

et al. 2024

Front. Plant Sci.

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The knowledge of pollen morphology, suitable storage condition, and species compatibility is vital for a successful grapevine improvement programme. Ten grape genotypes from three different species, viz., Vitis vinifera L., Vitis parviflora Roxb., and Vitis champini Planc., were studied for their pollen structure and pollen storage with the objective of determining their utilization in grape rootstock improvement programs. Pollen morphology was examined through the use of a scanning electron microscope (SEM). The viability of the pollen was assessed using 2,3,5-triphenyltetrazolium chloride (TTC). In vitro pollen germination was investigated using the semi-solid medium with 10 % sucrose, 100 mg/L boric acid, and 300 mg/L calcium nitrate. The results revealed variations in pollen micro-morphology in 10 genotypes, with distinct pollen dimensions, shapes, and exine ornamentation. However, species-wise, no clear difference was found for these parameters. Pollen of V. parviflora Roxb. and Dogridge was acolporated and did not germinate. The remaining eight genotypes exhibited tricolporated pollen and showed satisfactory in vitro pollen germination. Storage temperature and duration interactions showed that, at room temperature, pollen of most of the grape genotypes can be stored for up to 1 day only with an acceptable pollen germination rate (>30 %). However, storage for up to 7 days was successfully achieved at 4 °C, except for ‘Pearl of Csaba’. The most effective storage conditions were found to be at −20 °C and −196 °C (in liquid N2), enabling pollen storage for a period of up to 30 days, and can be used for pollination to overcome the challenge of asynchronous flowering. Four interspecific combinations were studied for their compatibility, among which V. parviflora Roxb. × V. vinifera L. (Pusa Navrang) and V. parviflora Roxb. × V. champini Planc. (Salt Creek) showed high cross-compatibility, offering their potential use for grape rootstock breeding. However, V. parviflora Roxb. × V. vinifera L. (Male Hybrid) recorded the lowest compatibility index among studied crosses. In the case of self-pollinated flowers from V. parviflora Roxb. and V. parviflora Roxb. × V. champini Planc. (Dogridge), pollen failed to germinate on the stigma due to male sterility caused by acolporated pollen. As a result, the flowers of these genotypes functioned as females, which means they are ideal female parents for grape breeding without the need for the tedious process of emasculation.

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Joy and Venus seedless grape cultivars in the lower Don Region

Krasokhina

2023

Sadovodstvo i vinogradarstvo

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In 2018-2022, a series of studies was conducted in the experimental plots of the Novocherkassk branch of Potapenko All-Russian Scientific Research Institute of Viticulture and Winemaking (Novocherkassk, Rostov Oblast) using conventional viticulture methods and GOSTs. A comparative study of the Joy grape, a new promising resistant seedless cultivar of the University of Arkansas (USA), and the Venus grape, one of the first seedless cultivars of the same institution, was carried out. The growing characteristics included the following: uncovered, unwatered, grafted, Berlandieri×Riparia Kober 5B rootstock, 3.0×1.5 m planting scheme, fan-shape formation on the middle stem. Agrobiological indicators, phenological observations, productivity, chemical and technological data, as well as tasting evaluations of fresh and dried grapes, are presented. During the study period, the preservation of buds in the Joy and Venus plants was 78.3 % and 80.1 %, respectively. Although the indicators of shoot fruitfulness and fruiting ratio were high in both cultivars, Joy showed a higher average weight of bunches and berries compared to Venus (311 and 282 g; 3.1 and 1.6 g, respectively). The yield was 13.8 t/ha and 12.4 t/ha, respectively. Both cultivars proved to be highly resistant to major grape diseases; in 2021, Joy grape bunches were slightly affected by mildew at the level of 2.5 points. The mass concentration (average for the study period) of sugars in Joy and Venus plants comprised 20.2 g/100 cm3 under the titratable acidity of 6.8 g/dm3 and 19.3 g/100 cm3 under the titratable acidity of 7.3 g/dm3, respectively. The conclusion is made that the studied cultivars present interest for cultivation as table wine and raisin varieties in areas with sufficiently moderate climate due to their high yield and seedless characteristics. These cultivars are recommended for possible expansion of vine plantations and further use in breeding.

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Development of Abiotic Stress Resistant Grapevine Varieties

Cited by 3 publications

References 379 publications

Worthy Micropropagation Protocols of Seven Cultivars of Pomegranate (Punica Granatum L.) Cultivars in the Province of Duhok, Kurd Istan Rigion of Iraq.

Worthy Micropropagation Protocols of Seven Cultivars of Pomegranate (Punica Granatum L.) Cultivars in the Province of Duhok, Kurd Istan Rigion of Iraq.

Studies on pollen micro-morphology, pollen storage methods, and cross-compatibility among grape (Vitis spp.) genotypes

Joy and Venus seedless grape cultivars in the lower Don Region

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