Development of AC microelectrophoresis for rapid protein affinity evaluation

Novel Microcantilever Design for Versatile Mass Sensor Application

“…Although the strength of the static charge interaction between an amino group and a carboxyl group differs according to the applied pH value, a significant interaction was possible under the current experimental condition (PBS buffer, pH = 7 4, 25 C), as confirmed by a previous experiment. 29 …”

“…In the current condition, both the rapid interaction and gradual interaction between the beads and cantilever are possible. 29 Another difference in the response spectra for the two cantilevers was the washing effect, as shown in Figures 7(a and b).…”

“…The cantilever-bead interaction is based on a static charge interaction mechanism. 29 The standard resonant frequency of the commercial cantilever was measured at 127 kHz, while that of the comb-type cantilever was measured at 87 kHz in the same buffer solution (PBS, pH = 7 4, 25 C). Thus, when looking at these standard frequency values, it would appear that the commercial cantilever had a better sensitivity than the comb-type cantilever because the higher oscillation frequency of the commercial cantilever represents a higher Q-factor value.…”

Photoluminescence enhancement of dye-doped nanoparticles by surface plasmon resonance effects of gold colloidal nanoparticles

“…The 100 nm Cy3 dye-doped polystyrene nanoparticles (DDPNPs) were purchased from Invitrogent (CA, USA). These beads have pendent carboxylic acids, making them suitable for covalent coupling of proteins and other amine-containing biomolecules [10][11][12]. The concentration of the studied beads in the mother solution is 3.64 × 10 14 (ml) −1 .…”

“…The 100 nm Cy3 dye-doped polystyrene nanoparticles (Cy3 DDPNPs) used in this work are orange fluorescent 100 nm carboxylate-modified FluoSpheres R beads (Invitrogent), which have an absorption peak at 540 nm and emission peak at 560 nm. These nanoparticles have many biological applications such as super-resolution imaging of cells, analysis of interaction between biomolecules and fluorescence laser tracking [10][11][12]. Gold colloidal nanoparticles were added to Cy3 DDPNP solution to investigate the luminescence intensity affected by GNPs.…”