Development of accurate classification method based on the analysis of volatile organic compounds from human exhaled air

Berkel, Joep J B N van; Dallinga, Jan W.; Møller, Grith; Godschalk, Roger; Moonen, E.J.C.; Wouters, E.F.M.; Schooten, Frederik J. van

doi:10.1016/j.jchromb.2007.11.008

Cited by 132 publications

(141 citation statements)

References 17 publications

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“…Subjects were asked to inhale deeply and, subsequently, to exhale into resistance-free Tedlar bags (5 L) as previously described [13,16,17]. One to three exhalations were usually sufficient to inflate the bag.…”

Section: Exhaled Breath Sample Collectionmentioning

confidence: 99%

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Exhaled volatile organic compounds predict exacerbations of childhood asthma in a 1-year prospective study

et al. 2013

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The hypothesis was that prediction of asthma exacerbations in children is possible by profiles of exhaled volatile organic compounds (VOCs), a noninvasive measure of airway inflammation. The aims of the present study were to determine: 1) whether VOCs in exhaled breath are able to predict asthma exacerbations; and 2) the time course and chemical background of the most predictive VOCs.A prospective study was performed in 40 children with asthma over 1 year. At standard 2-month intervals, exhaled nitric oxide fraction (FeNO), VOC profiles in exhaled breath samples, lung function and symptoms were determined in a standardised way. VOC profiles were analysed by gas chromatographytime-of-flight mass spectrometry.16 out of 40 children experienced an exacerbation. With support vector machine analysis, the most optimal model of baseline measurements versus exacerbation within patients was based on six VOCs (correct classification 96%, sensitivity 100% and specificity 93%). The model of baseline values of patients with compared to those without an exacerbation consisted of seven VOCs (correct classification 91%, sensitivity 79% and specificity 100%). FeNO and lung function were not predictive for exacerbations.This study indicates that a combination of different exhaled VOCs is able to predict exacerbations of childhood asthma. @ERSpublications Exhaled volatile organic compounds predict exacerbations of childhood asthma in a 1-year prospective study

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Section: Exhaled Breath Sample Collectionmentioning

confidence: 99%

“…sampling time and reproducibility of the procedure are given elsewhere [16,17]. The contents of the bag were transferred under standardised conditions onto stainless steel two-bed sorption tubes containing active carbon (Markes International, Llantrisant, UK).…”

Section: Exhaled Breath Sample Collectionmentioning

confidence: 99%

Exhaled volatile organic compounds predict exacerbations of childhood asthma in a 1-year prospective study

et al. 2013

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“…There have been reports on the intra-individual variation of exhaled breath over a period of time [23]. This is an important parameter to be assessed for the developed methodology to verify if the individual profile is repeatable or consistent over time.…”

Section: Intra-individual Variability Evaluationmentioning

confidence: 99%

Profiling allergic asthma volatile metabolic patterns using a headspace-solid phase microextraction/gas chromatography based methodology

Caldeira

Barros

Bilelo³

et al. 2011

Journal of Chromatography A

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a b s t r a c tAllergic asthma represents an important public health issue with significant growth over the years, especially in the paediatric population. Exhaled breath is a non-invasive, easily performed and rapid method for obtaining samples from the lower respiratory tract. In the present manuscript, the metabolic volatile profiles of allergic asthma and control children were evaluated by headspace solid-phase microextraction combined with gas chromatography-quadrupole mass spectrometry (HS-SPME/GC-qMS). The lack of studies in breath of allergic asthmatic children by HS-SPME led to the development of an experimental design to optimize SPME parameters. To fulfil this objective, three important HS-SPME experimental parameters that influence the extraction efficiency, namely fibre coating, temperature and time extractions were considered. The selected conditions that promoted higher extraction efficiency corresponding to the higher GC peak areas and number of compounds were: DVB/CAR/PDMS coating fibre, 22 • C and 60 min as the extraction temperature and time, respectively. The suitability of two containers, 1 L Tedlar ® bags and BIOVOC ® , for breath collection and intra-individual variability were also investigated. The developed methodology was then applied to the analysis of children exhaled breath with allergic asthma (35), from which 13 had also allergic rhinitis, and healthy control children (15), allowing to identify 44 volatiles distributed over the chemical families of alkanes (linear and ramified) ketones, aromatic hydrocarbons, aldehydes, acids, among others. Multivariate studies were performed by Partial Least Squares-Discriminant Analysis (PLS-DA) using a set of 28 selected metabolites and discrimination between allergic asthma and control children was attained with a classification rate of 88%. The allergic asthma paediatric population was characterized mainly by the compounds linked to oxidative stress, such as alkanes and aldehydes. Furthermore, more detailed information was achieved combining the volatile metabolic data, suggested by PLS-DA model, and clinical data.

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“…The possibility of using breath tests to evaluate exposure to ETS is very promising given that it is a procedure that is safe and easy to perform. Preliminary studies have suggested that some VOCs detected in breath samples may be biomarkers (e.g., 1,3-butadiene, benzene, 2,5-dimethylfuran) [8][9][10][11][12][13][14][15][16].…”

mentioning

confidence: 99%

“…The simplicity and high sensitivity of the capillary TD module developed and the ability to determine the presence of VOCs at sub-ppbv levels in both environmental air and The possible use of 2,5-dimethylfuran as a smoking biomarker has also been proposed in other previous studies [8,[14][15][16]. However, the reduced number of samples evaluated in these studies makes them insufficient for adequate statistical analysis and, furthermore, there is the need for a clinical study to test this hypothesis and the utility of the compound as a useful smoking status biomarker.…”

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confidence: 99%

Capillary thermal desorption unit for near real-time analysis of VOCs at sub-trace levels. Application to the analysis of environmental air contamination and breath samples

Alonso¹,

Castellanos²,

Martín³

et al. 2009

Journal of Chromatography B

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Please cite this article as: M. Alonso, M. Castellanos, J. Martín, J.M. Sanchez, Capillary thermal desorption unit for near real-time analysis of VOCs at sub-trace levels. Application to the analysis of environmental air contamination and breath samples, Journal of Chromatography B (2008Chromatography B ( ), doi:10.1016Chromatography B ( /j.jchromb.2009 This is a PDF file of an unedited manuscript that has been accepted for publication. As a service to our customers we are providing this early version of the manuscript. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final form. Please note that during the production process errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain.Page 1 of 32 A c c e p t e d M a n u s c r i p t 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60 61 62 63 64 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60 61 62 63 64

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Development of accurate classification method based on the analysis of volatile organic compounds from human exhaled air

Cited by 132 publications

References 17 publications

Exhaled volatile organic compounds predict exacerbations of childhood asthma in a 1-year prospective study

Exhaled volatile organic compounds predict exacerbations of childhood asthma in a 1-year prospective study

Profiling allergic asthma volatile metabolic patterns using a headspace-solid phase microextraction/gas chromatography based methodology

Capillary thermal desorption unit for near real-time analysis of VOCs at sub-trace levels. Application to the analysis of environmental air contamination and breath samples

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