Development of Active Specific Immunotherapeutic Agents Based on Cancer-Associated Mucins

Samuel, John; Longenecker, B. M.

doi:10.1007/978-1-4615-1823-5_39

Cited by 15 publications

(9 citation statements)

References 65 publications

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“…with 1 x 10 6 cells of the Lewis lung carcinoma cell line transfected with MUC1 antigen (LLC-MUC1). 19,20 Murine mammary carcinoma model. BALB/c mice preimmunized with C. crescentus were challenged with 1 x 10 6 EMT-6 cells subcutaneously (s.c.) into the nape of the neck on day 0.…”

Section: Fluorescence Activated Cell Sorting (Facs)mentioning

confidence: 99%

Anti-tumor effects of the bacterium caulobacter crescentus in murine tumor models

Bhatnagar

Awasthi²,

Nomellini³

et al. 2006

Cancer Biology & Therapy

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Caulobacter crescentus is a gram negative, non-pathogenic bacterium, common in aquatic and soil environments. One feature of note is a protein surface layer (S-layer) composed of a single protein, organized as a self-assembled crystalline array that coats the bacterium. In the course of efforts to express cancer-associated peptides as genetic insertions into the S-layer, we noted a tumor suppressive effect of the unmodified bacterium. C. crescentus was examined for anti-tumor activity against three transplantable tumor mouse models: Lewis lung carcinoma cells transfected with the MUC1 gene in C57BL/6, murine mammary carcinoma (EMT-6) in BALB/c (both in prophylactic and therapeutic mode) and murine leukemia cells (L1210) in DBA2. Mice were immunized three times i.p. with C. crescentus (2 x 10 7 cells/mouse). In prophylactic mode, the mice were challenged with tumor cells two weeks after the last immunization. Immunization with live C. crescentus resulted in anti-tumor activity in all three transplantable tumor models, as measured by prolonged survival, reduced tumor mass or reduced number of lung nodules, compared to saline control groups. In the Lewis lung and the EMT-6 mammary carcinoma murine models the number of lung nodules as well as the tumor weight was lower in mice treated with C. crescentus, compared to the control group; for EMT-6, this was observed in prophylactic and therapeutic modes. In the murine leukemia and Lewis lung carcinoma models prolonged survival was observed in the groups of mice immunized with Caulobacters. In most cases the live C. crescentus cells were markedly more efficacious than heat killed or formalin fixed cells, despite the fact that they do not grow or persist in mice. The results suggest that C. crescentus may be a safe, bacterial immunomodulator for the treatment of tumors.

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Section: Fluorescence Activated Cell Sorting (Facs)mentioning

confidence: 99%

Anti-tumor effects of the bacterium caulobacter crescentus in murine tumor models

Bhatnagar

Awasthi²,

Nomellini³

et al. 2006

Cancer Biology & Therapy

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“…The broad expression and high specificity of TF expression in cancer have led to many investigations into its potential use in cancer diagnosis [4,7,12] and cancer immunotherapy [26,[29][30][31][32][33]. All humans have naturally occurring antibodies against TF that occur after weaning and are probably induced by their own intestinal flora, which contains many strains of bacteria that express TF [6,27].…”

Section: Introductionmentioning

confidence: 99%

The oncofetal Thomsen–Friedenreich carbohydrate antigen in cancer progression

2007

Glycoconj J

168

158

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The oncofetal Thomsen-Friedenreich carbohydrate antigen (Galbeta1-3GalNAcalpha1-Ser/Thr TF or T antigen) is a pan-carcinoma antigen highly expressed by about 90% of all human carcinomas. Its broad expression and high specificity in cancer have attracted many investigations into its potential use in cancer diagnosis and immunotherapy. Over the past few years increasing evidence suggests that the increased TF occurrence in cancer cells may be functionally important in cancer progression by allowing increased interaction/communication of the cells with endogenous carbohydrate-binding proteins (lectins), particularly the members of the galactoside-binding galectin family. This review focuses on the recent progress in understanding of the regulation and functional significance of increased TF occurrence in cancer progression and metastasis.

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“…The mucins may also be recognized in an HLA-unrestricted fashion [8]. From preclinical studies, it became clear that remissions were obtainable by immunization [19,62] and that MUC-1 peptide protected mice from MUC-1-transfected tumors [18]. Moreover, with bacterial superantigenconjugated anti-MUC-1 antibodies (immunotoxins), lymphokine-activated killer (LAK)-cell-mediated MUC-1 specific cytotoxicity in mice was markedly enhanced [42].…”

Section: Mucin-based Vaccinationmentioning

confidence: 99%

Immunotherapy in pancreatic cancer - current status and future

Staib¹,

Link

Beger

1999

Langenbeck's Archives of Surgery

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Although single remarkable improvements in the immunological approach to treatment of pancreatic cancer have been made, immunotherapy in pancreatic cancer is still experimental. On the basis of reliable preclinical data, new immunotherapy protocols will have to be evaluated clinically. Careful monitoring of immune responses and side effects, and assessment of quality of life will ensure identification of effective immunotherapy protocols for human pancreatic cancer in the near future.

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Development of Active Specific Immunotherapeutic Agents Based on Cancer-Associated Mucins

Cited by 15 publications

References 65 publications

Anti-tumor effects of the bacterium caulobacter crescentus in murine tumor models

Anti-tumor effects of the bacterium caulobacter crescentus in murine tumor models

The oncofetal Thomsen–Friedenreich carbohydrate antigen in cancer progression

Immunotherapy in pancreatic cancer - current status and future

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