Development of Aflatoxin B1 Aptamer Sensor Based on Iron Porphyrin Organic Porous Material

Wang, Min; Duan, Mengting; Yu, Fengxian; Fu, Xuewen; Gu, MengQiao; Chi, Kuan‐Neng; Mei, Li; Xia, Xiaojuan; Hu, Rong; Yang, Yunhui; Meng, Shuang

doi:10.1007/s12161-020-01877-2

Cited by 15 publications

(4 citation statements)

References 30 publications

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“…Wang [38] and colleagues utilized molybdenum disulfidedoped gold nanoclusters (AuNC@MoS2) as a substrate, with streptavidin-modified iron porphyrin organic porous materials loaded on AuNC@MoS2 as labels, to construct an electrochemical aptasensor based on AuNBPs@PAF-40-Fe for detecting AFB 1 in soy milk. This allowed for the quantitative detection of AFB 1 .…”

Section: Afb1 and Afm1 In Soy And Dairy Productsmentioning

confidence: 99%

Research Progress on Electrochemical Sensors based on Metal-Organic Framework Materials for the Detection of Mycotoxins in Food

Yang

2024

AJST

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Mycotoxins, with their teratogenic, carcinogenic, and mutagenic effects, pose a significant threat to food safety, socio-economic development, and human health. They are commonly found in cereal crops at concentrations that are difficult to detect, necessitating rapid, sensitive, and accurate detection methods. Metal-Organic Framework (MOF) boasts advantages such as a high specific surface area, high porosity, tunable structures, good conductivity, and stability, making them widely used as excellent metallic materials. Recently, many have made progress in constructing electrochemical sensors based on MOF in combination with metal nanoparticles, porphyrins, polycyclic aromatic hydrocarbons, graphene, etc., for the detection of mycotoxins in food, achieving promising results. In this review, we discuss the harmful effects of mycotoxins, list the regulatory standards for mycotoxins in food set by different organizations and countries, and highlight the methods for detecting common mycotoxins in food using MOF-based electrochemical sensors, providing a reference for subsequent detection of mycotoxins in food.

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Section: Afb1 and Afm1 In Soy And Dairy Productsmentioning

confidence: 99%

Research Progress on Electrochemical Sensors based on Metal-Organic Framework Materials for the Detection of Mycotoxins in Food

Yang

2024

AJST

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“…In addition, in the last five years, macromolecules like Amyloid β peptide, [184] Aflatoxin B1, [185][186][187] Fumonisin B1, [188] Thrombin, [189,190] Human epidermal growth factor receptor 2 (HER2), [191,192] Carcinoembryonic Antigen (CEA), [193] and DNA [194][195][196][197][198][199][200] can also be sensed using metalloporphyrins.…”

Section: Macromoleculesmentioning

confidence: 99%

Versatile Metalloporphyrin‐Based Electrochemical Sensing Applications: From Small Gasotransmitters to Macromolecules

2023

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At the beginning of his youthful sixth decade, this work is dedicated to Prof. Dr. Fabio Doctorovich. He is distinctive in organometallic chemistry. During his successful career, he has been studying the reactivity and application of metalloporphyrins. Metalloporphyrins are organometallic complexes that exhibit, through synthetic modifications, the ability to tune their optical and electrochemical properties and their selectivity towards a particular molecule or ion. For this reason, they are systems extremely useful as electrochemical sensors to detect and quantify a wide variety of analytes with high selectivity, even in real samples such as food, water, biological fluids, or pharmaceutical compounds. This review presents an up-to-date list of reports in which metalloporphyrins are used as electrochemical sensors. In addition to compiling a comprehensive and up-to-date list of reported sensors that utilize metalloporphyrins, this work aims to provide an overview of currently available tools and techniques for the detection of various chemical species through similar approaches, which are constantly being developed.

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“…The traditional techniques currently used for AFB1 detection include thin-layer chromatography (TLC) [11], liquid chromatography-mass spectrometry (LCS) [12], and high-performance liquid chromatography (HPLC) [13]. These methods, however, are time-consuming and call for difficult pretreatment procedures, specialized and expensive equipment, and trained operational personnel [14]. These drawbacks prevent these techniques from being widely used and prevent them from meeting the demands of quick and inexpensive screening analyses.…”

Section: Introductionmentioning

confidence: 99%

A Label-Free Aptasensor for Turn-On Fluorescent Detection of Aflatoxin B1 Based on an Aggregation-Induced-Emission-Active Probe and Single-Walled Carbon Nanohorns

Yang,

Lv,

Niu

et al. 2023

Foods

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The determination of the aflatoxin B1 (AFB1) content has received widespread attention in the context of food safety, which is a global public health issue. Accordingly, a label-free and turn-on fluorescent AFB1 determination method is developed herein with an ss-DNA aptamer as the recognition element, 4, 4-(1E,1E)-2, 2-(anthracene-9, 10-diyl) bis(ethene-2, 1-diyl) bis(N, N, N-trimethylbenzenaminium iodide) (DSAI) as the aggregation-induced emission (AIE) fluorescent probe, and single-walled carbon nanohorns (SWCNHs) as the selective part with a fluorescence quenching effect. In the presence of AFB1, the AFB1-specific aptamer undergoes a structural transformation and switches from being a random helix to a folded structure. DSAI’s fluorescence is protected as a result of the resistance of the transformed aptamer adsorbed on the SWCNHs’ surface. Because DSAI’s fluorescence is not quenchable, the fluorescence intensity is calculated as a function of the AFB1 concentration. By simply mixing DSAI, aptamer, AFB1 samples, and SWCNHs, the method can be carried out in 2 h, with a limit of detection (LOD) of 1.83 ng/mL. It has a high selectivity in the presence of other mycotoxins, and its performance is confirmed in soybean sauce with a known concentration of AFB1. The LOD was 1.92 ng/mL in the soy sauce samples and the recovery ranged from 95 to 106%, implying that the presented aptasensor has great potential for food analysis.

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Development of Aflatoxin B1 Aptamer Sensor Based on Iron Porphyrin Organic Porous Material

Cited by 15 publications

References 30 publications

Research Progress on Electrochemical Sensors based on Metal-Organic Framework Materials for the Detection of Mycotoxins in Food

Research Progress on Electrochemical Sensors based on Metal-Organic Framework Materials for the Detection of Mycotoxins in Food

Versatile Metalloporphyrin‐Based Electrochemical Sensing Applications: From Small Gasotransmitters to Macromolecules

A Label-Free Aptasensor for Turn-On Fluorescent Detection of Aflatoxin B1 Based on an Aggregation-Induced-Emission-Active Probe and Single-Walled Carbon Nanohorns

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