Development of amperometric biosensor in modified carbon paste with enzymatic preparation based on lactase immobilized on carbon nanotubes

Brito, Aila Riany de; Reis, Nadabe dos Santos; Oliveira, Polyany Cabral; Rezende, Denilde Vilas Bôas; Monteiro, Gabriel Pereira; Soares, Glêydison Amarante; Jesus, Rodrigo Sá de; Santos, Antonio S.; Salay, Luiz Carlos; Oliveira, Julieta Rangel de; Franco, Marcelo

doi:10.1007/s13197-019-04168-9

Cited by 18 publications

(5 citation statements)

References 36 publications

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“…Without statistical fitting of the data to Hill's model, it would be possible to work with different linear concentration ranges, but this procedure would make the dynamic range very narrow (saturation of the biosensor's surface at [lactose] > 100 nmol L −1 was observed; Figure 5B), requiring sample dilution or pre-concentration steps that often lead to systematic errors in the results. Still, the sensitivity found with ABL/PMB/FTO was much higher than those reported for biosensing systems developed with cellobiose dehydrogenase (LOD = 3.5 mmol L −1 [53]), lactase (LOD = 100 µmol L −1 [54]), β-galactosidase/glucose oxidase (LOD = 0.23 µmol L −1 [28]), and β-galactosidase/glucose oxidase/peroxidase (LOD = 0.46 µmol L −1 [55]), although all demonstrated analytical efficiency for different purposes. The fitting of data to Hill's equation further extended the biosensor's applicability at higher concentrations.…”

Section: Biosensor Analytical Performancementioning

confidence: 64%

Agaricus bisporus Wild Mushroom Extract as Lectin Source for Engineering a Lactose Photoelectrochemical Biosensor

et al. 2023

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Agaricus bisporus mushroom biomass contains a lectin, ABL, with remarkable specificity for lactose biorecognition; in this work, this feature was explored to develop a photoelectrochemical biosensor. The high lectin activity found in saline extracts of this macrofungus (640 HU mL−1), even at critical pH values (4–10) and temperatures (20–100 °C), allowed its direct use as an ABL source. Theoretical and experimental evidence revealed favorable electrostatic and biocompatible conditions to immobilize ABL on a poly(methylene blue)/fluorine-doped tin oxide-coated glass platform, giving rise to the ABL/PMB/FTO biosensor. The conducting polymer added further photoactivity to the device, allowing the identification of lectin–carbohydrate interactions with even greater sensitivity. The dose–response curves studied by electrochemical impedance spectroscopy showed a sigmoidal profile that was well-fitted by Hill’s equation, expanding the working dynamic range (15–540 nmol L−1 lactose; 20.2 pmol L−1 detection limit) and avoiding undesirable sample dilution or preconcentration procedures. Under the optimized photoelectrochemical conditions, the ABL/PMB/FTO biosensor showed remarkable signal stability, accuracy, specificity, and selectivity to analyze lactose in commercial food products. This research raises interest in ABL-based biosensors and the added value of the crude Agaricus bisporus extract toward the development of greener and more sustainable biotechnological approaches.

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Section: Biosensor Analytical Performancementioning

confidence: 64%

Agaricus bisporus Wild Mushroom Extract as Lectin Source for Engineering a Lactose Photoelectrochemical Biosensor

et al. 2023

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“…Lactose, the major disaccharide in milk and dairy products, is formed by the β-1,4glycosidic bond between galactose and glucose, and as a carbohydrate substitute, the sensitive detection of lactose content in food is an important factor in human health management [67,68]. It was found that carbon nanotubes interact well with lactase (LAC), and the biosensor obtained by immobilizing LAC with CNT had a sensitivity of up to 5.67 µA cm −2 mmol −1 L, with a limit of detection of about 100 × 10 −6 mol L −1 ; and the stability of the system was improved with the introduction of CNT as, after about 12 h of use, the current signal did not change after about 12 h of use [69]. Building on this, the team further used only CNT as a substrate to immobilize LAC by adsorption without any polymer stabilization layer or external membrane for the rapid and sensitive detection of lactose in skimmed milk samples [70].…”

Section: Other Enzymes For the Development Of Electrochemical Biosensorsmentioning

confidence: 99%

Immobilization of Enzyme Electrochemical Biosensors and Their Application to Food Bioprocess Monitoring

Sun,

Fang,

Liu

et al. 2023

Preprint

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Electrochemical biosensors based on immobilized enzymes are among the most popular and commercially successful biosensors. Literature in this field suggests that modification of electrodes with nanomaterials is an excellent method for enzyme immobilization, which can greatly improve the stability and sensitivity of the sensor. However, the poor stability, weak reproducibility, and limited lifetime of the enzyme itself still limit the requirements for the development of enzyme electrochemical biosensors for food production process monitoring. Therefore, constructing sensing technologies based on enzyme electrochemical biosensors remains a great challenge. This article outlines the construction principles of four generations of enzyme electrochemical biosensors and discusses the applications of single-enzyme systems, multi-enzyme systems and nano-enzyme systems developed based on these principles. The article further describes methods to improve enzyme immobilisation by combining different types of nanomaterials such as metals and their oxides, graphene-related materials, metal-organic frameworks, carbon nanotubes and conducting polymers. In addition, the article highlights the challenges and future trends of enzyme electrochemical biosensors, providing theoretical support and future perspectives for further research and development of high-performance enzyme chemical biosensors.

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“…Lactose, the major disaccharide in milk and dairy products, is formed by the β-1,4-glycosidic bond between galactose and glucose, and as a carbohydrate substitute, the sensitive detection of lactose content in food is an important factor in human health management [ 67 , 68 ]. It was found that carbon nanotubes interact well with lactase (LAC), and the biosensor obtained by immobilizing LAC with CNT had a sensitivity of up to 5.67 μA cm −2 mmol −1 L, with a limit of detection of about 100 × 10 −6 mol L −1 ; and the stability of the system was improved with the introduction of CNT as, after about 12 h of use, the current signal did not change after about 12 h of use [ 69 ]. Building on this, the team further used only CNT as a substrate to immobilize LAC by adsorption without any polymer stabilization layer or external membrane for the rapid and sensitive detection of lactose in skimmed milk samples [ 70 ].…”

Section: Enzymatic Electrochemical Biosensors For Food Bioprocess Mon...mentioning

confidence: 99%

Immobilization of Enzyme Electrochemical Biosensors and Their Application to Food Bioprocess Monitoring

Sun,

Wei,

Zhang

et al. 2023

Biosensors

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Electrochemical biosensors based on immobilized enzymes are among the most popular and commercially successful biosensors. The literature in this field suggests that modification of electrodes with nanomaterials is an excellent method for enzyme immobilization, which can greatly improve the stability and sensitivity of the sensor. However, the poor stability, weak reproducibility, and limited lifetime of the enzyme itself still limit the requirements for the development of enzyme electrochemical biosensors for food production process monitoring. Therefore, constructing sensing technologies based on enzyme electrochemical biosensors remains a great challenge. This article outlines the construction principles of four generations of enzyme electrochemical biosensors and discusses the applications of single-enzyme systems, multi-enzyme systems, and nano-enzyme systems developed based on these principles. The article further describes methods to improve enzyme immobilization by combining different types of nanomaterials such as metals and their oxides, graphene-related materials, metal–organic frameworks, carbon nanotubes, and conducting polymers. In addition, the article highlights the challenges and future trends of enzyme electrochemical biosensors, providing theoretical support and future perspectives for further research and development of high-performance enzyme chemical biosensors.

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Development of amperometric biosensor in modified carbon paste with enzymatic preparation based on lactase immobilized on carbon nanotubes

Cited by 18 publications

References 36 publications

Agaricus bisporus Wild Mushroom Extract as Lectin Source for Engineering a Lactose Photoelectrochemical Biosensor

Agaricus bisporus Wild Mushroom Extract as Lectin Source for Engineering a Lactose Photoelectrochemical Biosensor

Immobilization of Enzyme Electrochemical Biosensors and Their Application to Food Bioprocess Monitoring

Immobilization of Enzyme Electrochemical Biosensors and Their Application to Food Bioprocess Monitoring

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