Development of an Agrobacterium-mediated transformation method for Taxus suspension cultures

Using the Strategy of Inducing and Genetically Transforming Plant Suspension Cells to Produce High Value-Added Bioactive Substances

“…Specific substances produced in the injured parts of dicotyledonous plants can promote agrobacterium accumulation; however, phenolic substances need to be added in the culture process to achieve foreign gene transformation in monocotyledonous plants. Foreign genes can be stably integrated into the nuclear genome and expressed in plant cells for the long-term. , Agrobacterium transformation, the most widely used genetic transformation method at present, has been widely applied and has achieved the genetic transformation of the plant callus, , leaves, hypocotyls, suspension cells, and other materials. Jun Yang’s team constructed a recombinant plasmid with the uidA gene as a visible marker.…”

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“…Genetic manipulation tools have offered researchers a handle to shift from cutting down trees to PTX production in cell suspension cultures. Agrobacterium mediated transformation of taxus cell in suspension cultures has been one way towards developing high yield cell cultures for large scale biomanufacturing [2]. However, setting up in vitro cultures of Taxus species is anything but straightforward and the search for high yielding cell cultures is on.…”

“…These are the technology drivers of bioMEMS fabrication techniques. Researchers have leveraged these principles to build new and emerging technology platforms such as organ-on-a-chip and tissue engineering for applications in next generation regenerative medicine [2][3][4][5][6].…”

“…However, the challenges of achieving a seamless integration of bioMEMS devices include interfacing electronics with a human body with minimized dimension, weight, and power consumption. It also requires the device to be safe and reliable along with circuits to be functional in all situations including harsh and humid environment [2].…”

The effect of L-glutamine on the genetic transformation of embryogenic cell suspensions of gentian species (Gentiana lutea L., Gentiana cruciata L., and Gentiana kurroo Royle) using Agrobacterium tumefaciens

“…The embryogenic cell suspensions were utilized in vector transformation experiments in species belonging to different taxonomic clades, for example, Carica papaya, Hordeum vulgare, Nicotiana tabacum, Santalum album, and four species of Taxus taxa (Ketchum et al, 2007;Shekhawat et al, 2008;Kim et al, 2004;Wu et al, 1998;Carlos-Hilario and Christopher, 2015;Wilson et al, 2018). Owing to its economic value, special attention was paid to the transformation of embryogenic cell suspension of various cultivars of Musa acuminata, such as "Mas", "Rasthali", "Robusta" and "Dwarf Caven" (Ganapathi et al, 2001;Huang et al, 2007;Chee Wong et al, 2008;Ghosh et al, 2009;Chong-Rerez et al, 2012).…”