Development of an All-Marine 3D Printed Bioactive Hydrogel Dressing for Treatment of Hard-to-Heal Wounds

Stenlund, Patrik; Enstedt, Linnea; Gilljam, Karin M.; Standoft, Simon; Ahlinder, Astrid; Johnson, Maria Lundin; Lund, Henrik; Millqvist‐Fureby, Anna; Berglin, Mattias

doi:10.3390/polym15122627

Cited by 10 publications

(3 citation statements)

References 56 publications

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“…Thus, it does not require the use of specific equipment, which is used in the method of using particular hydrogel compositions as ink for 3D photoprinting of biologically active hydrogel materials [23] and does not include the stage of post-processing of annual products [24].…”

Section: Discussionmentioning

confidence: 99%

Modeling of Smart Bio-Medical Active Polymeric Hydrogel Transdermal Materials

Lebedeva,

Cherkashina,

Masikevych

et al. 2024

JES

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In this article, effective 3D printing modeling technology of smart bio-medical polymeric hydrogel transdermal materials based on gelatin and sodium alginate, modified by humic acids, was researched. Such smart biologically active polymeric hydrogel materials showed interesting applicability in tissue engineering fields due to their intrinsic biological compatibility, adaptability, and capacity to replicate the extracellular matrix environment. A literature review was carried out and proved that 3D printing modeling technology is a perspective for the functional effect on the smart bio-medical polymer hydrogel transdermal properties. Smart biomedical polymeric transdermal hydrogel patches were produced using a micromolding technique. A stereolithography (SLA) 3D printer was used to print the master mold. The three-stage technology of lignite humic acids modification of smart biologically active polymeric hydrogel transdermal microneedles patches based on gelatin, hydroxypropyl methylcellulose, and sodium alginate was designed. It was shown that modification of gelatin-sodium alginate and hydroxypropyl methylcellulose-sodium alginate biopolymer hydrogels by humic acids makes it possible to obtain smart biologically active polymeric hydrogel transdermal materials with an increased swelling degree and ability to improve the skin moisture-lipid balance (from the initial moisture 34–36 % and fatness 8–10, they increase to 58–66 % and 52–60 %). Finally, the developed 3D printing modeling technology of smart bio-medical polymeric hydrogel transdermal materials hydrogel based on gelatin sodium alginate, modified by humic acids, is a transdermal material with required properties.

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Section: Discussionmentioning

confidence: 99%

Modeling of Smart Bio-Medical Active Polymeric Hydrogel Transdermal Materials

Lebedeva,

Cherkashina,

Masikevych

et al. 2024

JES

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“…Numerous studies have shown significant improvements in healing post-injury in animal models with dressings infused with different bioactive molecules, such as alginate from seaweed [79], silicate bioactive glass [80], thrombomodulin [81] and arginine derivatives [82]. With the time related information shown in lipid profiles in Figure 4, it is apparent that the phospholipids and HDL subfraction predominant lipid and lipoprotein profiles in the delayed re-epithelisation group remained constant after surgical wound debridement and became more pronounced at two weeks post-surgery.…”

Section: Discussionmentioning

confidence: 99%

Clinical prediction of wound re-epithelisation outcomes in non-severe burn injury using the plasma lipidome

Ryan,

Raby,

Masuda

et al. 2023

Preprint

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Impaired wound healing in burn injuries can lead to complications such as skin graft loss, infection, and increased risk of scarring, which impacts long-term patient outcomes and quality of life. While wound repair in severe burns has received substantial research attention, poor wound outcomes in cases of non-severe burns (classified as <20% of the total body surface area (TBSA)) remain relatively understudied despite also having considerable physiological impact and constituting the majority of hospital admissions for burns. Predicting outcomes in the early stages of healing would decrease financial and patient burden, and aid in preventing long-term complications from poor wound healing. Lipids have been implicated in inflammation and tissue repair processes and may play essential roles in burn wound healing. Longitudinal plasma samples were collected from patients (n=20) with non-severe (<15% TBSA) flame or scald burns over a 6-week period including timepoints pre- and post-surgical intervention. Samples were analysed using liquid chromatography-tandem mass spectrometry and nuclear magnetic resonance spectroscopy to detect 850 lipid species and 112 lipoproteins. Statistical analyses, including orthogonal projection to latent structures-discriminant analysis was performed to identify changes associated with either re-epithelialisation or delayed wound re-epithelisation. The results demonstrated that the plasma lipid and lipoprotein profiles at admission could predict wound re-epithelisation outcomes at two weeks post-surgery, and that these discriminatory profiles were maintained over a 6-week period. Triacylglycerides, diacylglycerides (DAG) and low density lipoprotein (LDL) subfractions were associated with delayed wound closure, with DAG(18:2_18:3) and LDL/High density lipoprotein (HDL) ratio having the most influence (p-value < 0.02, Cliff's delta > 0.7), while HDL subfractions, phosphatidylinositols, phosphatidylcholines (PC), and phosphatidylserines were associated with re-epithelisation at two weeks post-surgery, with PC(16:0_18:1) and HDL-2 apolipoprotein-A1 showing the greatest influence on the model (p-value < 0.01 , Cliff's delta < -0.7). We demonstrate clinical prediction of wound re-epithelisation in non-severe burn patients using lipid and lipoprotein profiling. Further validation of the models will potentially lead to personalised intervention strategies to enhance injury outcomes, reducing the risk of chronic complications post-burn injury.

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“…35 To our knowledge, it is crucial to possess both contact and release antibacterial capabilities to achieve lasting and durable effects for an ideal antibacterial wound dressing. [36][37][38] Additionally, their preparation, architecture, biocompatibility, biodegradability, encapsulation property, and intelligence need to be considerations. [39][40][41] In this study, we supposed that developing a wound dressing incorporating glycyrrhizic acid (GA) and WS-CQDs could serve as an effective strategy for improving the MRSA-infected wound healing process.…”

Section: Introductionmentioning

confidence: 99%

Synergistic and Long-Lasting Wound Dressings Promote Multidrug-Resistant Staphylococcus Aureus-Infected Wound Healing

Fu¹,

Ni²,

Wang³

et al. 2023

IJN

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Background Multidrug-resistant staphylococcus aureus infected wounds can lead to nonhealing, systemic infections, and even death. Although advanced dressings are effective in protecting, disinfecting, and maintaining moist microenvironments, they often have limitations such as single functionality, inadequate drug release, poor biosafety, or high rates of drug resistance. Methods Here, a novel wound dressing comprising glycyrrhizic acid (GA) and tryptophan-sorbitol carbon quantum dots (WS-CQDs) was developed, which exhibit synergistic and long-lasting antibacterial and anti-inflammatory effects. We investigated the characterization, mechanical properties, synergistic antibacterial effects, sustained-release properties, and cytotoxicity of GA/WS-CQDs hydrogels in vitro. Additionally, we performed transcriptome sequence analysis to elucidate the antibacterial mechanism. Furthermore, we evaluated the biosafety, anti-inflammatory effects, and wound healing ability of GA/WS-CQDs dressings using an in vivo mouse model of methicillin-resistant staphylococcus aureus (MRSA)-infected wounds. Results The prepared GA/WS-CQDs hydrogels demonstrated superior anti-MRSA effects compared to common antibiotics in vitro. Furthermore, the sustained release of WS-CQDs from GA/WS-CQDs hydrogels lasted for up to 60 h, with a cumulative release of exceeding 90%. The sustained-released WS-CQDs exhibited excellent anti-MRSA effects, with low drug resistance attributed to DNA damage and inhibition of bacterial biofilm formation. Notably, in vivo experiments showed that GA/WS-CQDs dressings reduced the expression of inflammatory factors (TNF-α, IL-1β, and IL-6) and significantly promoted the healing of MRSA-infected wounds with almost no systemic toxicity. Importantly, the dressings did not require replacement during the treatment process. Conclusion These findings emphasize the high suitability of GA/WS-CQDs dressings for MRSA-infected wound healing and their potential for clinical translation.

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Development of an All-Marine 3D Printed Bioactive Hydrogel Dressing for Treatment of Hard-to-Heal Wounds

Cited by 10 publications

References 56 publications

Modeling of Smart Bio-Medical Active Polymeric Hydrogel Transdermal Materials

Modeling of Smart Bio-Medical Active Polymeric Hydrogel Transdermal Materials

Clinical prediction of wound re-epithelisation outcomes in non-severe burn injury using the plasma lipidome

Synergistic and Long-Lasting Wound Dressings Promote Multidrug-Resistant Staphylococcus Aureus-Infected Wound Healing

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